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[1]李剑瑜,刘毅,高波,等.三七皂甙Rg1对脂多糖诱导的大鼠肝细胞损伤的防护作用[J].环球中医药,2012,5(01):19-0.
　LI Jian yu,LIU Yi,GAO Bo,et al.Investigation of the protective effect of panax Notoginseng saponins Rg1 on damage of isolated rat hepatocytes[J].,2012,5(01):19-0.
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三七皂甙Rg1对脂多糖诱导的大鼠肝细胞损伤的防护作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年01期
页码:
19-0
栏目:
论著
出版日期:
2012-01-06

文章信息/Info

Title:
Investigation of the protective effect of panax Notoginseng saponins Rg1 on damage of isolated rat hepatocytes
作者:
李剑瑜; 刘毅; 高波; 戴富林; 武凡;
Author(s):
LI Jianyu;  LIU Yi; GAO Bo; et al
Laboratory Department, Zhuozhou Hospital, Zhuozhou 072750,China
关键词:
脂多糖;  单细胞凝胶电泳;  三七皂甙Rg1;  肝细胞损害
Keywords:
Liposaccharide; Single cell gel electrophoresis; Panax notoginseng saponins Rg1; Liver damage
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的探讨脂多糖诱导大鼠肝细胞的损害特点,并探讨三七皂甙Rg1抗肝细胞损害的保护作用。方法用胶原酶灌流法分离肝细胞。脂多糖诱导大鼠肝细胞损害,透射电镜观察肝细胞形态学的变化,DNA凝胶电泳观察其生化变化,用单细胞凝胶电泳法(SCGE)进一步探讨肝细胞损害的特点,并探讨三七皂甙Rg1对肝细胞损害的保护作用。结果由脂多糖诱导的肝细胞在形态上表现出染色质凝聚、凋亡小体形成等凋亡特征,生化方面表现出典型的DNA梯度,此过程可被凋亡抑制剂Quinaceine抑制。用SCGE法检测出,肝细胞凋亡和死亡数量与脂多糖的诱导时间有明显的相关性。在此基础上,观察到三七皂甙Rg1可抑制肝细胞凋亡和坏死,其抑制效果与Quinaceine无明显差异。结论三七皂甙Rg1可显著抑制肝细胞凋亡和坏死,是防治肝细胞损害的良好药物。
Abstract:
ObjectiveTo investigate the effect of liposaccharide (LPS) on hepatocyte damage and find out effective hepatoprotectants by means of single cell gel electrophoresis. MethodsThe hepatocytes were isolated in the way of liver perfusion with collagenase. Transmission electron microscope technique was used to observe the morphological change of hepatocyte. The biochemical changes were analyzed by the DNA agarose gel electrophoresis. The SCGE was used to study hepatic damage and find out effective hepatoprotectants. Results The hepatocytes incubated with LPS exhibited the condensation of nuclear chromatin and formed apoptotic bodies.The DNA fragment was demonstrated in biochemistry, but it was inhibitory by Quinacrine. The rate of hepatic apoptosis and necrosis has a good conelationship with LPStreated time.Panax notoginseng saponins Rg1 can inhibit both of them. The inhibited effect had no difference with Quinacrine. ConclutionsPanax notoginseng saponins Rg1 can inhibit hepatic apoptosis and necrosis remarkebly, It has a good protective effect on damage of rat hepatocytes.

参考文献/References:

[1]武凡，张树三，康格非.三七皂甙对肝纤维化大鼠分泌型磷脂酶A2和肿瘤坏死因子表达的影响［J］.中华肝脏病杂志,2003,11(1):5152.
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备注/Memo

备注/Memo:

作者单位：072750河北省涿洲市医院检验科（李剑瑜、武凡）,呼吸科（刘毅、高波、戴富林）
作者简介：李剑瑜（1974- ），女，本科，主治检验师。研究方向：肝细胞的损害与抗损害。Email：ljy1797@163.com
通讯作者：武凡（1949- ），女，博士，教授。研究方向：肝细胞的损害与抗损害。Email:foxfoxzhou@sina.com
更新日期/Last Update: 1900-01-01