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[1]冯振宇,赵建平.疏风宣肺解毒汤抗甲1型流感病毒诱导细胞凋亡的研究[J].环球中医药,2012,5(02):88-0.
　FENG Zhen-yu,ZHAO Jian-ping..Study of Shufengxuanfeijiedu Decoction in inhibiting cell apoptosis induced by influenza virus A1[J].,2012,5(02):88-0.
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疏风宣肺解毒汤抗甲1型流感病毒诱导细胞凋亡的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年02期
页码:
88-0
栏目:
中药抗菌抗病毒研究
出版日期:
2012-02-06

文章信息/Info

Title:
Study of Shufengxuanfeijiedu Decoction in inhibiting cell apoptosis induced by influenza virus A1
作者:
冯振宇; 赵建平;
Author(s):
FENG Zhen-yu; ZHAO Jian-ping.
Department of Traditional Chinese Medicine, Shanxi Province Hospital on Integrated Chinese and Western medicine,Taiyuan 030013,China
关键词:
疏风宣肺解毒汤;  流感病毒;  犬肾传代细胞;  细胞凋亡
Keywords:
Shufengxuanfeijiedu Decoction;  Influenza virus;  Madin-Darby canine kidney cell;  Cell apoptosis
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的探讨疏风宣肺解毒汤对甲1型流感病毒感染的犬肾传代细胞凋亡的影响。方法将犬肾传代细胞分为六组:正常细胞对照组、病毒对照组、疏风宣肺解毒汤治疗组、疏风宣肺解毒汤对照组、奥司他韦治疗组、奥司他韦对照组,含药组均加入相应的最大无毒限量药液,Annexin V和PI双染用流式细胞仪(flow cytometry,FCM)测定细胞凋亡的百分率。结果疏风宣肺解毒汤治疗组和奥司他韦治疗组的凋亡率显著低于病毒对照组(P<0.01),而高于其药物对照组(P<0.01); 疏风宣肺解毒汤对甲1流感病毒诱导的细胞凋亡的抑制率(IR)为67.07%,奥司他韦为76.36%。结论疏风宣肺解毒汤可抵抗甲1型流感病毒诱导的细胞凋亡,作用与奥司他韦相近。
Abstract:
Objective To investigate the effects of Shufengxuanfeijieduedu Decoction on the apoptosis of Madin-Darby canine kidney(MDCK)cells infected by influenza virus A1. Methods MDCK cells was were divided into 6 groups:control group of normal cells,virus control group,Shufengxuanfeijieduedu Decoction treatment group,Shufengxuanfeijieduedu Decoction control group,Oseltamivir treatment group,Oseltamivir control group.Each group were added to the highest non-toxic doses of the corresponding liquide.The percentage of apoptotic MDCK cells was calculated through flow eytometry by Annexin V and PI co-dyeing.Results Shufengxuanfeijieduedu Decoction treatment group and Oseltamivir treatment group were significantly lower than the virus control group(P<0.01),but higher than their corresponding drug control group(P<0.01); the inhibition rate(IR)of influenza virus A1 induced MDCK cells apoptosis of Shufengxuanfeijieduedu Decoction was 67.07%,while the IR of Oseltamivir was 76.36%.Conclusion The results of the present study suggested that Shufengxuanfeijieduedu Decoction resist against the apoptosis induced by influenza virus A1 and have comparable effect to Oseltamivir.

参考文献/References:

[1] Takieuusa T,Matsukawa S, Higuchi Y, et al.Induction of programmed cell death(apoptosis)by influenza virus infection in tissue culture ce11s[J].J Gen Virol,1993,74( Pt 11):2347.
[2] Mori I,Komatsu T,Takeuchi K,et a1.In vivo induction of apoptosis byinfluenza virus[J].J Gen Viral,1995,76(11):2869-2873.
[3] Toshihiro I,Kobayashi Y,Morita T,et a1.Virulent influenza A viruses induCe apoptosis in chickens[J].Virus Research,2002,84(1-2):27-35.
[4] Hinshaw VS,Olsen CW,Dybdahl-Sissoko N,et a1. Apoptosis:a mechanism of cell killing by influenza A and B viruses[J].J Viral,1994,68(6):3667-3673.

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备注/Memo

备注/Memo:
基金项目:国家中医药管理局传染病防治行业专项(200907001-2); 山西省科技攻关项目(20090312020-3)
作者单位：030013太原,山西省中西医结合医院中医科
作者简介：冯振宇(1974- ),硕士,主治医师。研究方向:中医外感病证研究。E-mail:sxfzy@163.com
更新日期/Last Update: 1900-01-01