|本期目录/Table of Contents|

[1]姜楠,梁晓春,屈岭,等.筋脉通胶囊对糖尿病大鼠坐骨神经形态测量学及核转录因子κB蛋白表达的影响[J].环球中医药,2012,5(02):100-0.
　JIANG Nan,LIANG Xiao-chun,QU Ling,et al.Effects of Jinmaitong Capsule on morphology and expression of nuclear factor kappa B of sciatic nerve in diabetic rats[J].,2012,5(02):100-0.
点击复制

筋脉通胶囊对糖尿病大鼠坐骨神经形态测量学及核转录因子κB蛋白表达的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年02期
页码:
100-0
栏目:
论著
出版日期:
2012-02-06

文章信息/Info

Title:
Effects of Jinmaitong Capsule on morphology and expression of nuclear factor kappa B of sciatic nerve in diabetic rats
作者:
姜楠; 梁晓春; 屈岭; 吴群励; 孙连庆; 赵丽;
Author(s):
JIANG Nan; LIANG Xiao-chun; QU Ling; et al.
Department of Traditional Chinese Medicine,Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China
关键词:
糖尿病周围神经病变;  核转录因子κB;  形态测量学;  筋脉通
Keywords:
Diabetic peripheral neuropathy;  Nuclear factor kappa B;  Morphology;  Traditional Chinese medicine Jinmaitong Capsule
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的观察中药筋脉通胶囊对糖尿病大鼠坐骨神经形态测量学及核转录因子κB(nuclear factor kappa B,NF-κB)蛋白表达的影响。方法采用链脲佐菌素糖尿病大鼠模型,随机分为模型组和筋脉通组及神经妥乐平组,设立正常对照组。灌胃16周后取坐骨神经进行形态测量学分析,免疫组化法测定其NF-κB蛋白表达。结果与正常对照组相比,模型组大鼠的有髓神经纤维密度、小纤维密度减少,小纤维比率下降,有髓神经纤维平均横截面积、轴索平均横截面积增大,差异显著(P<0.01); 筋脉通组、神经妥乐平组的有髓神经纤维密度、小纤维密度、小纤维比率均较模型组显著改善(P<0.05); 筋脉通组的有髓神经纤维平均横截面积、轴索平均横截面积较模型组改善显著(P<0.05)。模型组大鼠NF-κB蛋白的表达较正常组显著升高(P<0.01); 筋脉通组、神经妥乐平组上值均较模型组降低(P<0.05)。结论筋脉通胶囊可改善糖尿病大鼠坐骨神经的形态测量学异常,减少NF-κB的异常高表达。
Abstract:
Objective To study the effects of Jinmaitong Capsule(JMT)on morphology and expression of nuclear factor kappa B(NF-κB)of sciatic nerves in streptozotocin-induced diabetic rats. Methods Streptozotocin-induced diabetic rats were randomly divided into 3 groups including model group(DM group), JMT group and Neurotropin group(Ntp group).Normal rats were nsed as normal control group.All rats were given intragastric administration for 16 weeks.The morphometric studies of sciatic nerves were investigated.I immunohistochemical method was applied to detect the NF-κB's expression in sciatic nerves.Results Compared with the normal control group,the density of myelinated fibers and small myelinated fibers,the rate of small myelinated fibers,the size of myelinated fibers and axon cross sections in the DM group were significantly reduced(P<0.01).The indexes above in 2 treatment groups were significantly improved compared with the DM group. The size of axon cross sections in the JMT group were more significantly improved than in the DM group(P<0.05). The NF-κB's expression in the DM group was significantly higher than in the normal control group(P<0.01), while which in treatment groups were lower than in the DM group(P<0.05). Conclusion JMT could improve the pathological morphology of sciatic nerves and down-regulate the expression of NF-κB in DPN rats.

参考文献/References:

[1] Bierhaus A, Haslbeck KM, Humpert PM, et al. Loss of pain perception in diabetes is dependent on a receptor of the immunoglobulin superfamily[J]. J Clin Invest,2004, 114(12): 1741-1751.
[2] Kang KA, Lee KH, Zhang R, et al.Protective effects of Castanopsis cuspidate through activation of ERK and NF-kappaB on oxidative cell death induced by hydrogen peroxide[J]. J Toxicol Environ Health A,2007,70(15-16):1319-1328.
[3] 屈岭,梁晓春,吴群励,等. 筋脉通对糖尿病大鼠周围神经组织神经生长因子表达的影响[J].中国中药杂志,2008,33(21):2539-2544.
[4] 潘长玉 译.Joslin糖尿病学[M].14版.北京:人民卫生出版社,2007:988.
[5] 梁晓春,张宏,郭赛珊,等.筋脉通对糖尿病大鼠坐骨神经传导速度、醛糖还原酶及山梨醇浓度的影响[J]. 中国糖尿病杂志,2000, 8(1):37-39.
[6] Murakawa Y, Zhang W, Pierson CR, et al.Impaired glucose tolerance and insulinopenia in the GK-rat causes peripheral neuropathy[J]. Diabetes Metab Res Rev, 2002, 18(6): 473-483.
[7] Sima AA, Zhang W, Xu G, et al.A comparison of diabetic polyneuropathy in type II diabetic BBZDR/Wor rats and in type I diabetic BB/Wor rats[J]. Diabetologia, 2000, 43(6): 786-793.
[8] 何媛媛. 核因子κB与2型糖尿病关系的研究进展[J].国外医学老年医学分册,2007,28(5):220-225.
[9] 王燕燕,王杨天,狄红杰,等. 糖尿病大鼠坐骨神经的核因子-κB表达及干预[J]. 江苏医药,2006,32(7):645-647.
[10] Wang Y, Schmeichel AM, Iida H,et al.Enhanced inflammatory response via activation of NF-kappaB in acute experimental diabetic neuropathy subjected to ischemia-reperfusion injury[J]. J Neurol Sci, 2006, 247(1): 47-52.
[11] 陆邦超,赵明,王扬天,等. 核转录因子κB 在糖尿病大鼠坐骨神经中的表达及其与传导速度的关系[J]. 中国糖尿病杂志,2008,16(1):42-44.
[12] 梁晓春,崔丽英,郭赛珊,等. 筋脉通治疗糖尿病周围神经病变的临床观察[J].中国中西医结合杂志,1999,19(9):517-519.
[13] 张克俭,梁晓春,郭赛珊,等.筋脉通胶囊对糖尿病周围神经病变患者钠钾腺苷三磷酸酶活性的影响[J].中医杂志,2000,42(3):159-161.
[14] 郝伟欣,贾力,徐惠媛,等.筋脉通对大鼠坐骨神经传导速度及红细胞抗氧化作用的影响[J].中国新药杂志,2003,12(5):343-345.
[15] Qu Ling,Liang xiao-chun,Zhang Hong,et al. Effect of Jinmaitong with Medicated Serum on Proliferation of Rat Schwann Cells Cultured in High Glucose Medium[J], Chinese Journal of Integrative Medicine,2008,14(4):293-297.
[16] 孙连庆,梁晓春,张宏,等.中药筋脉通对高糖培养雪旺细胞增殖及NGF表达的影响[J].中华中医药杂志,2009,24(8):1019-1022.
[17] 王普艳,梁晓春,张宏,等.筋脉通胶囊对高糖培养雪旺细胞睫状体神经营养因子表达的影响[J].中医杂志,2010,51(8):736-739.

相似文献/References:

[1]王涵 周强 仝小林.仝小林治疗糖尿病并发症应用黄芪经验[J].环球中医药,2013,6(04):272.
[2]孙朦朦,刘苇苇,倪青,等.单味中药治疗糖尿病周围神经病变研究进展[J].环球中医药,2013,6(02):143.
　SUN Meng meng,LIU Wei wei,NI Qing..Research of single herbs in the treatment of Diabetic Peripheral Neuropathy[J].,2013,6(02):143.
[3]陈惠,刘苇苇,黄静,等.益气温阳通络法治疗糖尿病周围神经病变探讨[J].环球中医药,2012,5(09):686.
[4]孙青,梁晓春,张倩,等.糖尿病周围神经病变中医证型分析及其与交感神经皮肤反应的关系[J].环球中医药,2012,5(05):337.
　SUN Qing,LIANG Xiao chun,ZHANG Qian,et al.Clinical study on relationship between syndrome of traditional Chinese medicine and sympathetic skin response in diabetic peripheral neuropathy[J].,2012,5(02):337.
[5]张翔 梁晓春.补肾活血法治疗糖尿病周围神经病变的研究进展[J].环球中医药,2014,7(06):492.
　ZHANG Xiang,LIANG Xiao chun..Research progress in Bushen Huoxue treatment of diabetic peripheral neuropathies[J].,2014,7(02):492.
[6]钟柳娜.栗德林教授治疗糖尿病周围神经病变经验[J].环球中医药,2015,8(06):737.[doi:A doi:10.3969/j.issn.1674-1749.2015.06.029]
[7]熊广华,李怡.中药腿足浴治疗糖尿病周围神经病变研究概况[J].环球中医药,2015,8(05):609.[doi:10.3969/j.issn.16741749.2015.05.031]
　XIONG Guang hua,LI Yi..Research situation of treating diabetic peripheral neuropathy with traditional Chinese medicine leg and foot bathing therapy[J].,2015,8(02):609.[doi:10.3969/j.issn.16741749.2015.05.031]
[8]张岩,穆晓红,孙文,等.糖痹康对糖尿病大鼠坐骨神经II相代谢酶GCLc和GST pi蛋白与mRNA的影响[J].环球中医药,2016,9(03):283.[doi:10.3969/j.issn.1674-1749.2016.03.007]
　ZHANG Yan,MU Xiao hong,SU Wen,et al.Effect of Tangbikang on the protein and mRNA of Phase II metabolizing enzymes of GCLc and GST pi of sciatic nerve in diabetic rats[J].,2016,9(02):283.[doi:10.3969/j.issn.1674-1749.2016.03.007]
[9]杨丹,梁晓春,屈岭,等.中药筋脉通对糖尿病大鼠背根神经节神经元超微结构影响的研究[J].环球中医药,2016,9(06):654.[doi:10.3969/j.issn.1674-1749.2016.06.003]
　YANG Dan,LIANG Xiao-chun,QU Ling,et al.Effects of Jinmaitong on ultrastructure of DRG neurons in STZ-DM rats[J].,2016,9(02):654.[doi:10.3969/j.issn.1674-1749.2016.06.003]
[10]秦丽,窦永起,李敏,等.益气活血中药黄芪-丹参对脂多糖致急性肺损伤大鼠防治作用的机制研究[J].环球中医药,2017,10(01):2.[doi:10.3969/j.issn.1674-1749.2017.01.001]
　QIN Li,DOU Yongqi,LI Min,et al.Study the mechanism of supplementing qi and activating blood herbs on Lipopolysaccharide-induced acute lung injury in rats[J].,2017,10(02):2.[doi:10.3969/j.issn.1674-1749.2017.01.001]

备注/Memo

备注/Memo:
基金项目:北京市自然科学基金项目(7082077)
作者单位：100730 中国医学科学院 北京协和医学院 北京协和医院中医科(姜楠、梁晓春、屈岭、吴群励、孙连庆、赵丽); 北京中医药大学附属护国寺中医医院针灸科(姜楠)
作者简介：姜楠(1971- ),博士,主治医师。主要从事糖尿病慢性并发症的中西医结合临床研究。E-mail:jiangnanbj@yahoo.com.cn
通讯作者：梁晓春(1956- ),教授,博士生导师,中国中西医结合学会常务理事,中国中西医结合学会内分泌代谢病专业委员会副主任委员,北京中西医结合学会糖尿病专业委员会主任委员。研究方向:中西医结合防治糖尿病慢性并发症。E-mail:xcliang@vip.sina.com
更新日期/Last Update: 1900-01-01