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[1]龚誉华,程光宇,程为平,等.中药补脑止痫散对癫痫大鼠海马神经元凋亡基因Bax和Bcl-2表达的影响[J].环球中医药,2012,5(02):105-0.
　GONG Yu-hua,CHENG Guang-yu,CHENG Wei-ping..Effect of Bunaozhixian Powder on neuronal apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats[J].,2012,5(02):105-0.
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中药补脑止痫散对癫痫大鼠海马神经元凋亡基因Bax和Bcl-2表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年02期
页码:
105-0
栏目:
论著
出版日期:
2012-02-06

文章信息/Info

Title:
Effect of Bunaozhixian Powder on neuronal apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats
作者:
龚誉华; 程光宇; 程为平;
Author(s):
GONG Yu-hua;  CHENG Guang-yu;  CHENG Wei-ping.
Heilongjiang University of TCM, Harbin 150040, China
关键词:
癫痫;  神经元凋亡基因Bax和Bcl-2
Keywords:
Epilepsy;  Neuronal apoptosis genes Bax and Bcl-2
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的通过观察中药补脑止痫散对戊四氮化学点燃癫痫模型大鼠海马神经元凋亡基因Bax和Bcl-2表达的影响,探讨补脑止痫散治疗癫痫的相关机制。方法从78只正常Wistar大鼠中随机选取10只列为正常组,剩余68只大鼠腹腔注射戊四氮制作癫痫点燃模型,再随机选取50只造模成功大鼠随机分为5组:模型组、丙戊酸钠组、补脑止痫散高剂量组、补脑止痫散中剂量组和补脑止痫散低剂量组,每组10只。用免疫组化法检测药物治疗各组大鼠与正常组和模型组比较海马中神经元凋亡基因Bax和Bcl-2蛋白表达的变化。结果各治疗组大鼠海马Bax和Bcl-2蛋白阳性目标面密度值与模型组相比分别出现明显降低和升高(P<0.05),而补脑止痫散低剂量组大鼠海马Bax和Bcl-2蛋白阳性目标面密度值改变不明显(P>0.05),并且补脑止痫散高剂量组和中剂量组比较,以及此二组与丙戊酸钠组相比大鼠海马Bax和Bcl-2蛋白阳性目标面密度值改变均无明显差异(P>0.05)。结论中药补脑止痫散高、中剂量能调整癫痫患者海马神经元凋亡基因Bax和Bcl-2蛋白的表达,从而抑制癫痫发作。
Abstract:
Objective To explore the mechanism of the Bunaozhixian Powder on treatment of epilepsy, we observe the effect of Bunaozhixian Powder on neuron apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats induced by pentylenetetrazol(PTZ). Methods Select 10 rats from 78 normal wistar rats randomly as the normal group. The remainder 68 rats are injected abdominally with PTZ as epileptic model. Then randomly select 50 epileptic rats, divide these rats into 5 groups: the model group、the sodium valproate group、high dosage of Bunaozhixian Powder group、medium dosage of Bunaozhixian Powder group and low dosage of Bunaozhixian Powder group. Every group has 10 rats.5 groups are injected with PTZ as epileptic model except the normal group. Examine the hippocampus cerebral tissues Bax、Bcl-2 protein expression with enzyme immunoassay method in every group. Results Compared with the model group, the positive Bax and Bcl-2 protein density value of hippocampus neuron area of rats with medical treatment get significant lower and higher respectively(P<0.05),but low dosage of Bunaozhixian Powder group have no significant change(P>0.05).Besides, high dosage is compared to medium dosage of Bunaozhixian Powder group, and these two groups are compared to the sodium valproate group, the positive Bax and Bcl-2 protein density value have no significant change(P>0.05). Conclusion The high and medium dosage of Bunaozhixian Powder can change the neuronal apoptosis genes Bax and Bcl-2 expression in the hippocampus area of epileptic rats. So the Bunaozhixian Powder can inhibit epileptic seizures.

参考文献/References:

[1] 王焕明,谭启富,吴波,等. 颞叶癫痫患者脑内细胞凋亡现象的研究[J]. 中华神经外科杂志,1999,15(6):333-335.
[2] Korsmeyer SJ. Bcl-2 gene family and the regulation of programmed cell death[J]. Cancer Res,1999,59(7):1693-1699.
[3] Racine RJ.Modification of seizure activity by electrical stimulation:Ⅱ.Motor Seizure[J].Electroencephalogr Clin Neurophysiol,1972,32(3):281.
[4] 赵文娟,黄显奋. 癫痫中细胞凋亡发生的多元调节机制[J]. 国外医学:生理病理科学与临床分册,2000,20(1):18-21.
[5] Lei K,Davis RJ. JNK phosphorylation of Bim-related members of the Bcl-2 family induces Bax-dependent apoptosis[J]. Proc Natl Acad Sci USA,2003,100(5):2432-2437.
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备注/Memo

备注/Memo:
基金项目:哈尔滨市科技创新人才研究专项资金(优秀学科带头人项目)(2010RFXXS034)
作者单位：150040哈尔滨,黑龙江中医药大学[龚誉华(博士研究生)]; 香港浸会大学中医药学院(程光宇); 黑龙江中医药大学附属第一医院神经内科(程为平)
作者简介：龚誉华(1983- ),2009级在读博士研究生。研究方向:中西医结合治疗神经内科疾病。E-mail:scallen9401@sina.com
通讯作者：程为平(1958- ),主任医师,教授,博士生导师。研究方向:中西医结合治疗神经内科疾病。E-mail:cwp1958@163.com
更新日期/Last Update: 1900-01-01