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[1]冯婷,李峰,宋月晗,等.疏肝补肾法对睡眠剥夺大鼠学习记忆及前额叶皮质BDNF蛋白表达的影响[J].环球中医药,2012,5(04):255-0.
　FENG Ting,LI Feng,SONG Yue han,et al.Effects of learning and memory in rats with sleep deprivation and BDNF protein expression in the prefrontal cortex with soothing the liver and kidney method[J].,2012,5(04):255-0.
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疏肝补肾法对睡眠剥夺大鼠学习记忆及前额叶皮质BDNF蛋白表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年04期
页码:
255-0
栏目:
论著
出版日期:
2012-04-06

文章信息/Info

Title:
Effects of learning and memory in rats with sleep deprivation and BDNF protein expression in the prefrontal cortex with soothing the liver and kidney method
作者:
冯婷; 李峰; 宋月晗; 刘晓兰; 马捷; 毛萌; 吴凤芝; 刘晶;
Author(s):
FENG Ting;  LI Feng;  SONG Yuehan;  et al.
Departmen of TCM Diagnostics，Beijing University of Chinese Medicine,Beijing 100029,China
关键词:
睡眠剥夺;  疏肝补肾法;  脑源性神经营养因子;  超氧化物歧化酶;  Y迷宫
Keywords:
Sleep deprivation; Soothing the liver and tonifying the kidney; Brainderived neurotrophic factor（BDNF）; Superoxide dismutase（SOD）; Ymaz
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的研究疏肝补肾法对睡眠剥夺大鼠学习记忆及前额叶皮质脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)蛋白表达的变化。方法以成年雄性SD大鼠36只,随机分为对照组、模型组、疏肝补肾治疗组。应用168小时睡眠剥夺法复制模型,Y迷宫成绩评定学习记忆能力,以血清超氧化物歧化酶(super oxide dismutase,SOD)水平反映脑组织的损害。取大鼠前额叶皮质冰冻切片,以免疫组织化学法检测BDNF的蛋白表达。结果与对照组比较,模型组的Y迷宫成绩显著下降(P<0.01),血清SOD含量明显减少(P<0.01),前额叶皮质BDNF免疫阳性物明显减少(P<0.01); 与模型组比较,疏肝补肾治疗组Y迷宫成绩明显提高(P<0.01),血清SOD含量显著增多(P<0.01),BDNF蛋白表达也显著提高(P<0.01)。结论睡眠剥夺能造成加重脑损伤、学习记忆能力减退以及BDNF表达的减少,而疏肝补肾法能改善学习记忆和脑损伤,可能是通过上调BDNF蛋白的表达以及相关通路蛋白所致。
Abstract:
Objective To study changes of learning and memory and brainderived neurotrophic factor expression in the prefrontal cortex on rats with sleep deprivation through the method of soothing the liver and tonifying the kidney. Methods36 adult male SpargueDawley rats are randomly divided into control group, model group, and treated group. Applying the model of 168 hours sleep deprivation to reflect brain damage with levels of serum SOD and evaluate learning and memory abilities with the results of Ymaze. Using frozen sections in the prefrontal cortex of rats to detect protein expression of BDNF with immunohistochemical. Results Compared with the control group, Ymaze’s results reduced remarkably (P<0.01), serum SOD declined (P<0.01), and the immune reactive BDNF in prefrontal cortex declined (P<0.01) in the model group; compared with the model group, treated groups improved the Ymaze result (P<001), significantly increased serum SOD content (P<001), the expression of BDNF protein is also significantly enhanced (P<0.01). Conclusion Sleep deprivation can result in learning and memory loss and brain damage, reduction in the expression of BDNF; the method of soothing the liver and kidney can recover learning and memory and brain injury, possibly through increasing BDNF protein expression and relative pathways of protein.

参考文献/References:

[1]Machado RB, Hipólide DC, BeneditoSilva AA, et a1. Sleep deprivation induced by the modified multiple platform technique：quantification of sleep loss and recovery［J］. Brain Research, 2004, 1004(12):4551.
[2］王跃春,王子栋,孙黎明,等.动物学习记忆能力的Y型迷宫测试法［J］.暨南大学学报(自然科学与医学版),2001,22(5):137140.
[3］王秀利,唐洪丽,索国玲.癫痫持续状态对发育期大鼠学习记忆及海马磷酸化的CAMP反应元件结合蛋白表达的影响［J］.实用儿科临床杂志,2007,22(22):17231725.
[4］邱前程,卢善明,黄杰雄,等.不同固定剂对冰冻切片免疫组化结果的影响［J］.分子诊断与治疗杂志,2009,1(4):250252.
[5］Musumeci G, Sciarretta C, RodríguezMoreno A, et al. TrkB Modulates Fear Learning and Amygdalar Synaptic Plasticity by Specific Docking Sites［J］.J Neurosci,2009,29(32):1013110143.
[6］Poulin B, Butcher A, McWilliams P, et al. The M3muscarinic receptor regulates earning and memory in a receptor phosphorylation/arrestindependent manner［J］. Proc Natl Acad Sci USA,2010,107(20):94409445.
[7］Angelucci F, Mathé AA, Aloe L. Neurotrophic factors and CNS disorders: findings in rodent models of depression and schizophrenia［J］.Pro Brain Res,2004,146(3)：151165.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金面上项目（30873217）
作者单位：100029北京中医药大学中医诊断系[冯婷（博士研究生）、李峰、宋月晗、马捷（博士研究生）、毛萌（博士研究生）、吴凤芝（博士研究生）、刘晶（博士研究生）]，科研中心（刘晓兰）
作者简介：冯婷（1982- ），女，2009级在读博士研究生。研究方向：病证结合的辨证规律研究。Email：07fting@163.com
通讯作者：李峰（1966- ），博士，教授，博士生导师。研究方向：病证结合的辨证规律研究。Email：lifeng95@vip.sina.com
更新日期/Last Update: 1900-01-01