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[1]杨铂,刘靓雯,王小利,等.解毒护肝饮药物血清对异烟肼致HepG2坏死和凋亡的干预[J].环球中医药,2012,5(04):259-0.
　YANG Bo,LIU Jing wen,WANG Xiao li,et al.Effect of Jiedu Hugan Decoction containing serum on the death and apoptosis of HepG2 which were damaged by isoniazid[J].,2012,5(04):259-0.
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解毒护肝饮药物血清对异烟肼致HepG2坏死和凋亡的干预()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年04期
页码:
259-0
栏目:
论著
出版日期:
2012-04-06

文章信息/Info

Title:
Effect of Jiedu Hugan Decoction containing serum on the death and apoptosis of HepG2 which were damaged by isoniazid
作者:
杨铂; 刘靓雯; 王小利; 袁成民; 安勇;
Author(s):
YANG Bo; LIU Jingwen; WANG Xiaoli; et al.
Liver Disease Units,Ji’nan Infectious Hospital，Ji’nan 250021，China
关键词:
解毒护肝饮;  药物性肝炎;  异烟肼;  药物血清;  凋亡;  人肝癌细胞株;  体外培养;  Fas/FasL
Keywords:
Jiedu Hugan Decoction; Drug hepatitis; Isoniazid; Drug containing serum; Apoptosis; HepG2; Iv votro; Fas/FasL
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的 观察解毒护肝饮药物血清对异烟肼致人肝癌细胞株(human hepatocellular carcinoma cell lines,HepG2)病变的干预,并分析其机制。方法 制备解毒护肝饮和甘草酸二铵的药物血清,建立异烟肼致HepG2细胞损伤模型,以解毒护肝饮和甘草酸二铵药物血清加入HepG2培养液,观察对HepG2坏死和凋亡的干预,以及对HepG2 Fas/FasL表达的影响,以评价解毒护肝饮的疗效机制。结果 解毒护肝饮药物血清对异烟肼致HepG2损伤有明显抑制作用,可以减少HepG2细胞凋亡和坏死,并减少其Fas/FasL表达,效果优于甘草酸二铵胶囊组。结论 以清热利湿解毒兼养阴化瘀为治法的解毒护肝饮有确切的减少异烟肼致HepG2坏死和凋亡的作用,其抗细胞凋亡作用与减少HepG2的Fas/FasL的表达有关。
Abstract:
ObjectiveTo observe the effect of Jiedu Hugan Decoction containing serum on HepG2 damaged by isoniazid, and reveal the mechanism of it. MethodsEstablish iv votro HepG2 cell damaged by isoniazid culture experiment model. The serum was collected from the rats using Jiedu Hugan Decoction and Gancaosuaneran and incubated with the HepG2 model. After 24 hours, observe the death and apoptosis of HepG2 And the expressions of Fas/FasL were measured by flow cytometry. ResultsCulture experiment model of iv votro HepG2 cell damaged by isoniazid was established successfully. Jiedu Hugan Decoction containing serum significantly inhibited apoptosis and necrosis of the HepG2 cells and the expression of Fas/FasL. The curative effect of this is better than control group which were given Gancaosuaneran decoction containing serum. ConclusionJiedu Hugan Decoction according to qingre lishi jiedu can evidently reduce the hepatitis by decrease the expression of the factor associated with apoptosis. So it can improve the syndromes and experimental index of hepatitis induced by antitubercular drug.

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备注/Memo

备注/Memo:
基金项目：财政部、国家中医药管理局2009年中医药行业科研专项（2009070011）
作者单位：250021 济南市传染病医院（杨铂、刘靓雯、袁成民、安勇）；济南护理职业学院（王小利）
作者简介：杨铂（1979- ），硕士，主治医师。研究方向：中西医结合肝病传染病的防治。Email：optyb@sina.com
通讯作者：安勇（1967- ），硕士，主任医师。研究方向：中西医结合肝病传染病的防治。Email：anyong666@163.com
更新日期/Last Update: 1900-01-01