|本期目录/Table of Contents|

[1]李静,彭宁,刘勤社,等.活血胶囊对动脉粥样硬化兔平滑肌细胞凋亡及Bcl-2、Bax蛋白表达的影响[J].环球中医药,2012,5(07):506-0.
　LI Jing,PENG Ning,LIU Qin-she,et al.Effect of Huoxue Capsule on the apoptosis of vascular smooth muscle cell and expression of Bcl-2 and Bax protein on rabbits with atherosclerosis[J].,2012,5(07):506-0.
点击复制

活血胶囊对动脉粥样硬化兔平滑肌细胞凋亡及Bcl-2、Bax蛋白表达的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年07期
页码:
506-0
栏目:
论著
出版日期:
2012-07-06

文章信息/Info

Title:
Effect of Huoxue Capsule on the apoptosis of vascular smooth muscle cell and expression of Bcl-2 and Bax protein on rabbits with atherosclerosis
作者:
李静; 彭宁; 刘勤社; 周洁; 任得志;
Author(s):
LI Jing; PENG Ning; LIU Qin-she; et al.
Traditional Chinese Medicine department,Shaanxi Provincial People's Hospital,Xi'an 710068,China
关键词:
活血胶囊;  动脉粥样硬化;  平滑肌细胞凋亡;  Bcl-2;  Bax
Keywords:
Huoxue Capsule;  Atherosclerosis;  Smooth muscle cell apoptosis;  Bcl-2;  Bax
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的探讨活血胶囊干预对动脉粥样硬化兔主动脉平滑肌细胞凋亡及凋亡相关因子Bcl-2、Bax蛋白表达的影响。方法通过高脂饮食建立实验性动脉粥样硬化兔动物模型,采用TUNEL法检测平滑肌细胞凋亡,SP免疫组化法检测凋亡相关因子Bcl-2、Bax蛋白表达。结果与模型组比较,活血胶囊低、高剂量组均能降低兔主动脉平滑肌细胞凋亡的凋亡指数(P<0.05,P<0.01),降低Bax蛋白表达(P<0.05,P<0.01),升高Bcl-2蛋白表达(P<0.05,P<0.01),并呈现一定的量效关系(P<0.05)。结论活血胶囊可能通过上调Bcl-2蛋白表达,下调Bax蛋白表达,减少平滑肌细胞凋亡,从而抑制动脉粥样硬化斑块的形成。
Abstract:
Objective To observe the effect of Huoxue Capsule on the apoptosis of vascular smooth muscle cell(VSMC)and expression of Bcl-2 protein and Bax protein on rabbits with atherosclerosis. Methods The experimental model of rabbits with atherosclerosis were induced by atherogenic diet. The cell apoptosis were detected by TUNEL staining, and the expression of Bcl-2 protein and Bax protein were examined by SP immunohistochemical staining. Results Compared with the experimental model of rabbits, treatment with both high dose and low dose of Huoxue Capsule decreased the apoptotic index of VSMC and the expression of Bax protein(P<0.05,P<0.01), and increased the expression of Bcl-2 protein(P<0.05,P<0.01). In addition, the effect of treatment with high dose of Huoxue Capsule was better than with low dose of Huoxue(P<0.05). Conclusion Huoxue Capsule increased expression of Bcl-2 protein and decreased expression of Bax protein in rabbits with arteriosclerosis, and decreased the apoptosis of VSMC, which might be associated with its inhibiting effect on atherosclerotic plaque.

参考文献/References:

[1] Lusis AJ.Atherosclerosis [J]. Nature, 2000, 407(6801):233-241.
[2] Vannini N,Pfeffer U,Lorusso G,et al.Endothelial cell aging and apoptosis in prevention and disease: E-selectin expression and modulation as a model[J].Curr Pharm Des,2008,14(3):221-225.
[3] 周洁,刘勤社,张晓艳,等.活血胶囊对家兔动脉粥样硬化斑块稳定性的影响[J].中药新药与临床药理,2011,22(5):528-531.
[4] Sukhotnik I,Voskoboinik K,Lurie M, et al.Involvement of the bax and bcl-2 system in the induction of germ cell apoptosis is correlated with the time of reperfusion after testicular ischemia in a rat model[J].Fertil Steril,2009,92(4):1466-1469.
[5] 莫新民,曾英,侯斌,等.稳斑方对模型兔动脉粥样硬化斑块稳定性的影响[J].中医杂志,2009,50(8):740-742.
[6] 牛晓玲,孙志广,陆茵.活血化瘀中药抗动脉粥样硬化慢性炎症反应的研究进展[J].辽宁中医药大学学报,2009,11(9):36-38.

相似文献/References:

[1]李贞玉,杨惠民,郭杨志,等.血脂异常患者中医证型及血清sICAM-1、sVCAM-1水平与幽门螺旋杆菌感染的相关性研究[J].环球中医药,2015,8(02):137.[doi:10.3969/j.issn.1674-1749.2015.02.003]
　LI Zhen-yu,YANG Hui-min,GUO Yang-zhi,et al.Correlation study between the TCM syndromes of dyslipidemia, the level of serum sICAM-1,sVCAM-1 and helicobacter pylori infection[J].,2015,8(07):137.[doi:10.3969/j.issn.1674-1749.2015.02.003]
[2]杨祎,司晓雪,邵楚茜,等.健脾祛痰化瘀法防治动脉粥样硬化的临床研究进展[J].环球中医药,2015,8(09):1130.[doi:10.3969/j.issn.1674-1749.2015.09.030]
　YANG Yi,SI Xiao-xue,SHAO Chu-qian,et al.Advances in the clinical researches of treating atherosclerosis via the method of invigorating spleen, removing phlegm and eliminating blood statisis[J].,2015,8(07):1130.[doi:10.3969/j.issn.1674-1749.2015.09.030]
[3]郝蔷薇,麻春杰,乌兰其其格,等.额尔敦—乌日勒对动脉粥样硬化家兔血管内皮因子内皮素、血小板活化因子的影响[J].环球中医药,2015,8(12):1433.[doi:10.3969/j.issn.1674.1749.2015.12.003]
　HAO Qiang-wei,MA Chun-jie,WUlAN Qi-qige,et al.Research on the effect of Eerdun-wurili on rabbit atherosclerotic vulnerable plaque endothelial function[J].,2015,8(07):1433.[doi:10.3969/j.issn.1674.1749.2015.12.003]
[4]苏兆铎,郭书文,张璐,等.调脂通脉方对动脉粥样硬化兔模型血管重塑的影响[J].环球中医药,2017,10(04):430.[doi:10.3969/j.issn.1674-1749.2017.04.011]
　SU Zhaoduo,GUO Shuwen,ZHANG Lu,et al.Influences of Tiaozhi Tongmai formula on vascular remodeling in rabbits with atherosclerosis[J].,2017,10(07):430.[doi:10.3969/j.issn.1674-1749.2017.04.011]
[5]武迎磊 赵天 李明.灯盏花素对大鼠动脉粥样硬化保护作用的研究[J].环球中医药,2017,10(06):569.[doi:10.3969/j.issn.1674-1749.2017.06.007]
　WU Yinglei,ZHAO Tian,LI Ming..Protective effects and mechanism of breviscapine on atherosclerosis rats[J].,2017,10(07):569.[doi:10.3969/j.issn.1674-1749.2017.06.007]
[6]于少泓.马黛通络饮对APOE基因敲除小鼠脂质代谢的影响[J].环球中医药,2017,10(09):957.[doi:10.3969/j.issn.1674-1749.2017.09.006]
　YU Shaohong..Effects of Madai Tongluo decoction on lipid metabolism in apolipoprotein E-deficient mice[J].,2017,10(07):957.[doi:10.3969/j.issn.1674-1749.2017.09.006]

备注/Memo

备注/Memo:
基金项目:国家“十一五”科技支撑计划(2008BAI51B01); 陕西省“13115”科技创新工程重大科技专项计划(2009ZDKG-85)
作者单位：710068 西安,陕西省人民医院中医科(李静、彭宁、刘勤社、周洁); 陕西省中医医院心脏内科(任得志)
作者简介：李静(1975- ),硕士,主治医师。研究方向:中西医结合防治心脑血管疾病。E-mail:drli560@126.com
通讯作者：刘勤社(1963- ),主任医师,硕士研究生导师。研究方向:中西医结合临床和中药药理。E-mail:drlijing@126.com
更新日期/Last Update: 1900-01-01