|本期目录/Table of Contents|

[1]邱瑞桂,靳世英,徐和,等.薄膜分散联合冻融法制备小菜蛾抗菌肽脂质体研究[J].环球中医药,2012,5(12):904-0.
　QIU Rui-gui,JIN Shi-ying,XU He,et al.Preparation of pxCECA1 liposome by film dispersion method combined with freeze-thawing method[J].,2012,5(12):904-0.
点击复制

薄膜分散联合冻融法制备小菜蛾抗菌肽脂质体研究()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年12期
页码:
904-0
栏目:
论著
出版日期:
2012-12-06

文章信息/Info

Title:
Preparation of pxCECA1 liposome by film dispersion method combined with freeze-thawing method
作者:
邱瑞桂; 靳世英; 徐和; 徐平华; 靳士晓; 孟小林; 袁海龙; 韩晋;
Author(s):
QIU Rui-gui; JIN Shi-ying; XU He; et al.
Department of Medical Device,413 Military Hospital of PLA,Zhoushan 316000,China
关键词:
小菜蛾抗菌肽;  脂质体;  薄膜分散法;  冻融法;  包封率;  稳定性
Keywords:
pxCECA1;  Liposome;  Film dispersion method;  Freeze-thawing method;  Encapsulation efficiency;  Stability
分类号:
R943
DOI:
-
文献标志码:
A
摘要:
目的制备小菜蛾抗菌肽脂质体,提高小菜蛾抗菌肽的稳定性。方法采用薄膜分散联合冻融法制备小菜蛾抗菌肽脂质体,最终冷冻干燥成粉末。建立小菜蛾抗菌肽的HPLC含量测定方法,以包封率为主要指标,综合考虑冻融过程对包封率和粒径的影响,采用正交实验进行处方优化。并考察形态、粒径、包封率及不同储存温度下的稳定性。结果通过处方工艺优化制备的小菜蛾脂质体冻干粉呈球形或类球形,平均粒径为(223.1±31.2)nm,包封率为62%。本品在4℃下贮存3个月稳定,粒径及包封率无显著性变化。结论采用薄膜分散联合冻融法制备的小菜蛾抗菌肽脂质体能显著提高其长期贮存稳定性。
Abstract:
Objective To prepare Plutella xylostella pxCECA1(CA)liposomes and to enhance the stability of CA. Methods Liposomes of CA were prepared by film dispersion method combined with freeze-thawing method,and followed by lyophilization. The method for determination of CA by RP-HPLC was established. Orthogonal design was adopted to optimize the formulation using encapsulation efficiency as the evaluation parameter,and the influence of the freeze-thawing process on encapsulation efficiency and particle size was also taken into account. The morphous,particle size and the distribution,encapsulation efficiency,and stability of the products at different temperature were investigated. Results The results demonstrated that the prepared liposomes were spherical with the mean diameter of(223.1±31.2)nm and the mean encapsulation efficiency of 62%. CA liposomes were stable after being stored at 4 ℃ for 3 months,and no significant changes were found in particle size and encapsulation efficiency. Conclusion CA liposomes prepared by film dispersion method combined with freeze-thawing method exhibit greater stability.

参考文献/References:

[1] Hong Wang, Xiao-lin Meng, Jin-ping Xu, et al. Production, purification, and characterization of the cecropinfrom Plutella xylostella, pxCECA1, using an intein-induced self-cleavable system in Escherichia coli[J]. Appl Microbiol Biotechnol, 2012, 94(4): 1031-1039.
[2] Casteels P,Ampe C,Jacobs F,et al. Apidaecins:antibacterial peptides from honeybees[J]. The EMBO Journal,1989,8(8): 2387-2391.
[3] 朱雨晴,石森林. 中药脂质体稳定性的研究进展[J]. 药学学报,2009,44(11): 1211-1215.
[4] 刘晓谦,王锦玉,仝燕,等. 脂质体制备技术及其研究进展[J]. 中国药学杂志,2011,46(14): 1084-1088.
[5] 董泽民. 赖氨匹林脂质体鼻腔给药的研究[J]. 中国医药工业杂志,1995,26(5): 199-202.
[6] 张奇,邓英杰. 冻融法制备5-氟尿嘧啶脂质体及其稳定性考察[J]. 沈阳药科大学学报,2000,17(2): 87-89.
[7] 米宏杰,王瑞华,徐冰. 简述亲水性药物脂质体的制备[J]. 中华临床医学研究杂志,2006,12(16): 2219-2220.
[8] 王健,李明轩. 冷冻干燥对提高脂质体稳定性的研究概况[J]. 中国医药工业杂志,2005,36(9): 576-580.
[9] 邓礼荷,韦敏燕,汤晨懿,等. 冻干工艺及保护剂对羟基喜树碱脂质体质量的影响[J]. 中国医药工业杂志,2012,43(1): 30-34.

相似文献/References:

备注/Memo

备注/Memo:
基金项目:国家“重大新药创制”科技重大专项(2012ZX09J12108-04C); 北京市自然科学基金(7122176)
作者单位：316000浙江舟山,解放军第413医院药械科(邱瑞桂); 中国人民解放军第三〇二医院药学部[靳世英(硕士研究生)、徐和(硕士研究生)、徐平华(硕士研究生)、靳士晓(硕士研究生)、袁海龙、韩晋]; 武汉大学病毒学国家重点实验室(孟小林)
作者简介：邱瑞桂(1970- ),学士,副主任药师。研究方向:中药新型给药系统。E-mail:541277028@qq.com
通讯作者：袁海龙(1970- ),博士,研究员,硕士生导师。研究方向:中药新型给药系统研究。E-mail:yhlpharm@126.com; 韩晋(1959-),女,硕士,主任药师,硕士生导师。研究方向:中药剂型与工程技术研究。E-mail:hanjin302emba@163.com。袁海龙、韩晋并列为通讯作者。
更新日期/Last Update: 1900-01-01