|本期目录/Table of Contents|

[1]曹毅,翁鹤,杨晓红,等.高通量测序技术在黄连解毒汤抗白念珠菌机制研究中的应用及探讨[J].环球中医药,2013,6(01):55-0.
　CAO Yi,WENG He,YANG Xiao hong..Application and exploration of highthroughput sequencing thechnologies in mechanism study of HuanglianJiedu Decoction antagonize Candida albicans[J].,2013,6(01):55-0.
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高通量测序技术在黄连解毒汤抗白念珠菌机制研究中的应用及探讨()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年01期
页码:
55-0
栏目:
综述
出版日期:
2013-01-06

文章信息/Info

Title:
Application and exploration of highthroughput sequencing thechnologies in mechanism study of HuanglianJiedu Decoction antagonize Candida albicans
作者:
曹毅;翁鹤;杨晓红;
Author(s):
CAO Yi;  WENG He;  YANG Xiaohong.
Department of Dermatology, First Affiliated Hospital of Zhejiang Chinese Medicine University,Hangzhou 310006,China
关键词:
白念珠菌; 黄连解毒汤; 高通量测序技术; 应用探讨
Keywords:
Candida albicans; HuanglianJiedu Decoction; Highthroughput sequencing thechnology; Application and discussion
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
白念珠菌(Candidaalbicans)是临床上发生最为普遍的致病真菌,严重时可危及生命,目前主要以三唑类药物进行防治。但是由于药物长期广泛的使用及其作用位点的单一性,导致耐药问题日益突出。中药黄连解毒汤有良好的抑菌效果,并极大增强西药杀菌作用,但至今相关其分子机制的研究鲜有涉及。本文在阐述目前国内外现有高通量基因测序技术的基础上,重点探讨了高通量技术在黄连解毒汤抗白色念珠菌机制研究中的应用。
Abstract:
Candida albicans is the most common clinical pathogenic fungus and lifethreatening in serious situations. Triazole is the main drug used in clinic to prevent and treat individuals infected with Candida albicans presently. However, due to longterm extensive usage and single site of action, drug resistance problems have become increasingly prominent. Antimicrobial activity of HuanglianJiedu Decoction has been confirmed, and HuanglianJiedu Decoction has effective role in enhancing sterilization action of western medicine, but the molecular mechanism in which is rarely kown. In this article we discuss the current situation of the highthroughput sequencing thechnologies in the world, and focuse on the application and exploration of highthroughput sequencing thechnologies in the mechanism study of HuanglianJiedu Decoction antagonize Candida albicans.

参考文献/References:

［1］Arendrup.MC.Epidemiology of invasive candidiasis[J].Curr Opin Crit Care,2010,16(5):445452.
［2]Kothavade RJ,Oberai CM,Valand AG,et al.Disseminated cryptococcosis and fluconazole resistant oral candidiasis in a patient with acquired immunodeficiency syndrome (AIDS)[J].J Infect Dev Ctries,2010,4(10): 674678.
［3]Saunus JM,Kazoullis A,Farah CS.Cellular and molecular mechanisms of resistance to oral Candida albicans infections[J].Front Biosci,2008,13: 53455358.
［4]Wilson DT,Drew RH,Perfect JR.Antifungal therapy for invasive fungal diseases in allogeneic stem cell transplant recipients: an update[J].Mycopathologia,2009,168(6): 313327.
［5]Brito GN,Inocêncio AC,Querido SM.et al.In vitro antifungal susceptibility of Candida spp.oral isolates from HIVpositive patients and control individuals[J].Braz Oral Res,2011,25(1):2833.
［6]汪长中，程惠娟，官妍,等.黄连解毒汤对体外白念珠菌生物膜形成的影响[J].热带病与寄生虫学,2007,5(2): 8284.
［7]陶茂灿，夏修蛟，曹毅．黄连解毒汤体外抗真菌活性及其与西药的联合药敏试验研究[J].中华中医药学刊,2009,27(3): 585587.
［8]Holzknecht BJ,Thorup J,Arendrup MC,et al.Decreasing candidaemia rate in abdominal surgery patients after introduction of fluconazole prophylaxis[J].Clin Microbiol Infect,Clin Microbiol Infect,2011,17(9):13721380.
［9]MacCallum DM,Coste A,Ischer F,et al.Genetic Dissection of Azole Resistance Mechanisms in Candida albicans and Their Validation in a Mouse Model of Disseminated Infection[J].Antimicrob Agents Chemother,2010,54(4): 14761483.
［10]Sanglard D, Coste A, Ferrari S. Antifungal drug resistance mechanisms in fungal pathogens from the perspective of transcriptional gene regulation[J].FEMS Yeast Res,2009,9(7): 10291050.
［11]Coste A,Turner V,Ischer F,et al.A mutation in Tac1p,a transcription factor regulating CDR1 and CDR2,is coupled with loss of heterozygosity at chromosome 5 to mediate antifungal resistance in Candida albicans[J].Genetics,2006,172(4): 21392156.
［12]Dunkel N,Blass J,Rogers PD,et al.Mutations in the multidrug resistance regulator MRR1,followed by loss of heterozygosity,are the main cause of MDR1 overexpression in fluconazoleresistant Candida albicans strains[J].Mol Microbiol,2008,69(4): 827840.
［13]Coste A,Crittin J,Bauser C,et al.Functional analysis of cis and transacting elements of the Candida albicans CDR2 promoter with a novel promoter reporter system[J].Eukaryot Cell,2009,8(8): 12501267.
［14]Heilmann CJ,Schneider S,Barker KS,et al.An A643T Mutation in the transcription factor Upc2p causes constitutive ERG11 upregulation and increased fluconazole resistance in Candida albicans[J].Antimicrob Agents Chemother，2010,54(1): 353359.
［15]Wirsching S,Michel S,Morschhuser J.Targeted gene disruption in Candida albicans wildtype strains: the role of the MDR1 gene in fluconazole resistance of clinical Candida albicans isolates[J].Mol Microbiol，2000,36(4): 856865.
［16]Pinjon E,Moran GP,Jackson CJ,et al.Molecular mechanisms of itraconazole resistance in Candida dubliniensis[J].Antimicrob Agents Chemother，2003,47(8): 24242437.
［17]Morschhuser J.The genetic basis of fluconazole resistance development in Candida albicans[J].Biochemica et Biophysica Acta,2002,1587(23): 240248.
［18]Lamb DC,Kelly DE,White TC,et al.The R467K Amino Acid Substitution in Candida albicans Sterol 14αDemethylase Causes Drug Resistance through Reduced Affinity[J].Antimicrob Agents Chemother，2000,44(1): 6367.
［19]Marichal P, Vanden Bossche H, Odds FC. et al. Molecular biological characterization of an azoleresistant Candida glabrata isolate[J].Antimicrob Agents Chemother，1997,41(10): 22292237.
［20]Chau AS,Gurnani M,Hawkinson R,et al.Inactivation of sterol Δ5,6desaturase attenuates virulence in Candida albicans[J].Antimicrob Agents Chemother，2005,49(9): 36463651.
［21]Zhang H, Gao AL, Li FX, et al. Mechanism of action of tetrandrine, a natural inhibitor of Candida albicans drug efflux pumps[J].Yakugaku Zasshi，2009,129(5): 623633.
［22]Adams M, Mahringer A, Kunert O, et al. Cytotoxicity and pglycoprotein modulating effects of quinolones and indoloquinazolines from the Chinese herb Evodia rutaecarpa[J].Planta Med，2007,73(15): 15541557.
［23]Huang S,Cao YY,Dai BD,et al.In vitro synergism of fluconazole and baicalein against clinical isolates of Candida albicans resistant to fluconazole[J].Biol Pharm Bull，2008,31(12): 22342236.
［24]Cao YY,Dai BD,Wang Y,et al.In vitro activity of baicalein against Candida albicans biofilms[J].Int J Antimicrob Agents，2008,32(1): 7377.
［25]Liu X,Jiang JH,Shao JF,et al.Gene transcription profiling of Fusarium graminearum treated with an azole fungicide tebuconazole[J].Appl Microbiol Biotechnol，2010,85: 11051114.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（30471565）
作者单位：310006杭州,浙江中医药大学附属第一医院皮肤科[曹毅、翁鹤（硕士研究生）、杨晓红]
作者简介：曹毅（1965- ），博士，教授，主任医师，博士生导师。研究方向：皮肤科临床与科研。Email: caoyi1965@163.com
更新日期/Last Update: 1900-01-01