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[1]罗本华,张雪竹,赵岚,等.三种针法对快速老化痴呆模型小鼠老化度评分及行为学影响研究[J].环球中医药,2013,6(03):165-0.
　LUO Ben hua,ZHANG Xue zhu,ZHAO Lan,et al.Study of three aupuncture improving cognitive deficits in water maze and senescence score of SAMP8[J].,2013,6(03):165-0.
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三种针法对快速老化痴呆模型小鼠老化度评分及行为学影响研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年03期
页码:
165-0
栏目:
论著
出版日期:
2013-03-06

文章信息/Info

Title:
Study of three aupuncture improving cognitive deficits in water maze and senescence score of SAMP8
作者:
罗本华;张雪竹;赵岚;张荣超;韩景献;于建春;
Author(s):
LUO Benhua;  ZHANG Xuezhu;  ZHAO Lan;  et al.
Faculty of Zhuang Medicine of Guangxi University of Traditional Chinese Medicine, Nanning 530001, China
关键词:
快速老化痴呆模型小鼠(SAMP8); Morris水迷宫; 认知功能; 针灸疗法; 老化度评分
Keywords:
Senescenceaccelerated mice prone 8 (SAMP8); Morris water maze; Cognitive function; Acupuncture; Senescence score
分类号:
R254
DOI:
-
文献标志码:
A
摘要:
目的探讨三种针刺方法对快速老化痴呆模型小白鼠(senescence-acceleratedmiceprone8,SAMP8)认知功能的影响并进行针法优选。方法选取SAMP8小鼠60只随机分为SAMP8对照组、针刺腹穴组、针刺背俞穴组、针刺腹背穴组和针刺非穴组,每组12只,并设抗快速老化亚系小鼠(senescenceacceleratedmouseresistance1,SAMR1)对照组12只。针刺腹穴组采用"益气调血,扶本培元"针法,针刺膻中、中脘、气海及双侧血海、足三里;针刺背俞穴组采用双侧肺俞、脾俞和肾俞;针刺腹背穴组采用针刺腹穴加背穴组穴;针刺非穴组取双侧胁下两个固定非经非穴点;SAMP8对照组与SAMR1对照组均不做任何治疗。应用Morris水迷宫观测各组小鼠空间参考记忆能力的变化,分析针刺对SAMP8小鼠认知功能的影响。结果(1)三种针刺方法均能显著性降低老化度评分,而腹穴组和腹背穴针刺有肯定、显著的疗效优势。(2)SAMP8小鼠存在学习记忆的获得、巩固、保留和再学习方面的认知损害,而三个针刺方法都能显著地改善这种认知功能损害且具有穴位特异性;其中,在隐蔽平台中,针刺背俞穴组起效早但疗效不稳定,针刺腹背穴组起效稳定且从第三天保持最短的平均潜伏期时间;反向试验中,SAMP8针刺腹穴组显示更快和更稳定的作用。结论三种不同针法均能抵抗SAMP8快速老化进程,改善SAMP8认知功能障碍,而改善特点不同。动物实验支持原有"益气调血、扶本培元"针法处方。
Abstract:
ObjectiveStudy the effect of three acupuncture prescriptions on the behavior and appearance of senescence, the cognitive functions of alzheimer disease (AD) model mice and to select the optimal acupuncture method.MethodsTwelve male 8monthold senescence accelerated mouse resistance 1（SAMR1） and 60 male agematched senescenceaccelerated mice prone 8（SAMP8） were randomly divided into six groups: SAMR1 control group, SAMP8 control group, SAMP8 bellyacup, SAMP8 BackShuacup, SAMP8 bellybackacup and SAMP8 nonacupoint control group. The SAMP8 bellyacup group were given “Yiqitiaoxue(replenishing qi and regulating blood) and Fubenpeiyuan(reinforcing the antipathogenic qi and consolidating the body resistance)”acupuncture, the SAMP8 BackShuacup group were acupunctured at three BackShu acupoints, and the SAMP8 bellybackacup group were acupunctured at SAMP8 bellyacup groups and SAMP8 BackShuacup groups acupoints. The SAMP8 control group or SAMR1 control group mice were given only grasping treatment, and the SAMP8 nonacupoint control group mice were stimulated on the nonacupoint. Cognitive function was evaluated by the Morris water maze test, and the behavior and appearance of senescence was evaluated by the grading score of senescence system. ResultsThe data showed that SAMP8 displayed obvious impairments of acquisition, consolidation, retention and relearning ability of learning and memory and overall accelerated senescence characteristics; and three acupuncture methods could degree the senescence score and enhance all the aspects cognitive function of those impairments with acupointrelated specificity. Among acupuncture groups, the SAMP8 BackShuacup group took effects rapidly but unsteadily, the SAMP8 bellybackacup group took effects steadily with shortest time from third training day in the hidden platform trial, but the SAMP8 bellyacup group spent time least in the reversal trial. While in the test of spatial memory retention, either SAMP8 bellyacup group or SAMP8 bellybackacup group were better than SAMP8 BackShuacup group. ConclusionThe results suggested the three acupuncture methods could resist the process of senescence and improve the whole cognitive deficits of SAMP8 and influence the learning and memory abilities in different ways. In the whole, the study supports“Yiqitiaoxue and Fubenpeiyuan”acupuncture.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：天津市应用基础及前沿技术研究计划重点项目（11JCZDJC20100）；天津市高等学校科技发展基金（20090214）
作者单位：530001南宁，广西中医药大学壮医药学院（罗本华）；天津中医药大学第一附属医院针灸研究所（罗本华、张雪竹、赵岚、韩景献、于建春），分子生物学实验室（张雪竹、赵岚、于建春）；天津中医药大学［张荣超（研究生）］
作者简介：罗本华（1968-），博士，副主任医师。研究方向：针灸与壮医防治神经系统及老年性疾病研究。Email:luobenhua1968@163.com
通讯作者：于建春（1966-），博士，研究员。研究方向：脑老化及针刺防治的分子机理。Email: yujianchun2000@hotmail.com
更新日期/Last Update: 1900-01-01