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[1]隆献 何泽云 周艳利 罗寅亮 李岩岩 徐文峰 李旭华.六味地黄汤对5/6肾切除大鼠肾间质纤维化的 影响[J].环球中医药,2013,6(05):329-332.
　LONG Xian,HE Zhe yun,ZHOU Yan li,et al.Effect of Liuwei Dihuang Decoction on renal interstitial fibrosis in 5 /6 nephrectomized rat model[J].,2013,6(05):329-332.
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六味地黄汤对5/6肾切除大鼠肾间质纤维化的 影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年05期
页码:
329-332
栏目:
论著
出版日期:
2013-05-06

文章信息/Info

Title:
Effect of Liuwei Dihuang Decoction on renal interstitial fibrosis in 5 /6 nephrectomized rat model
作者:
隆献 何泽云 周艳利 罗寅亮 李岩岩 徐文峰 李旭华
作者单位：410007 长沙，湖南中医药大学第一附属医院肾内科［隆献(博士研究生)、何泽云、周艳利、罗寅亮、李岩岩、徐文峰(博士研究生)、李旭华(博士研究生)］；长沙民政职业技术学院医学院（隆献）
Author(s):
LONG Xian;  HE Zheyun;  ZHOU Yanli;  et al.
Nephrology department of the First Hospitalof Hunan University of Chinese Medcine, Changsha410007, China
Corresponding author: HE Zheyun, Email: hzy2005@zju.edu.cn
关键词:
六味地黄汤; 5/6肾切除; 肾间质纤维化; 肝细胞生长因子
Keywords:
Liuwei Dihuang Decoction; 5/6 nephrectomy; renal interstitial fibrosis; hepatocyte growth factor
分类号:
R285
DOI:
-
文献标志码:
-
摘要:
目的观察六味地黄汤对5/6肾切除大鼠肾间质纤维化的拮抗作用和对肝细胞生长因子（hepatocyte growth factor, HGF）及其特异性受体cMet表达的影响。方法SD大鼠分为假手术组、模型组和六味地黄汤组，采用5/6肾切除法复制慢性肾间质纤维化模型。造模1周后，灌胃干预8周。观察残肾病理改变，采用免疫组化检测肾组织中HGF和cMet蛋白的表达。结果模型组和六味地黄汤组残肾均出现肾间质纤维化，模型组更为严重；与假手术组相比，模型组HGF表达升高（P＜005），六味地黄汤组表达也升高（P＜001），六味地黄汤组HGF表达强于模型组（P＜005）；与假手术组相比，模型组和六味地黄汤组cMet表达均升高（P＜001），六味地黄汤组表达强于模型组（P＜001）。结论六味地黄汤可能通过促进5/6肾切除大鼠肾组织HGF及其受体cMet的表达，减轻肾间质纤维化程度。
Abstract:
ObjectiveTo observe the protection of Liuwei Dihuang Decoction on renal interstitial fibrosis, and to investigate its effects on hepatocyte growth factor (HGF) and its receptor (cMet) in 5/6 nephrectomy rats. MethodsSD rats were divided into sham operation group，model group and Liuwei Dihuang Decoction group, and adopted 5/6 nephrectomy to copied chronic renal interstitial fibrosis. A week after operation，rats were administrated every day for 8 weeks. Pathological changes of the remnant kidney were observed by HE and Masson staining, Immunohistochemistry was used to observe the expression of HGF and cMet protein. Resultsthe model group and the Liuwei Dihuang Decoction group both appeared renal fibrosis, the model group was more obvious; Compared with the sham operation group, the expression of HGF manifested obvious promotion in the model group (P＜0.05)，the expression in the Liuwei Dihuang Decoction group raised too (P＜0.01), compared with the model group, the expression in the Liuwei Dihuang Decoction group were more serious (P＜0.05); Compared with the sham operation group, the expression of cMet manifested obvious promotion in the model group and the Liuwei Dihuang Decoction group (P＜0.01), compared with the model group, the expression in the Liuwei Dihuang Decoction group manifested more serious (P＜0.01). ConclusionLiuwei Dihuang Decoction may promote the expression of HGF and cMet in 5/6 nephrectomy rats and inhibit the development of renal interstitial fibrosis.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：湖南省高校创新平台课题（09K059）；湖南省教育厅科研课题（12C0938）
作者简介：隆献（1979-），女，在读博士研究生，讲师。研究方向：慢性肾脏病的防治。Email: longx2003@sina.cn
通讯作者：何泽云（1962-），博士，教授。研究方向：慢性肾脏病的防治。Email: hzy2005@zju.edu.cn
更新日期/Last Update: 2013-05-31