|本期目录/Table of Contents|

[1]靳取,邓悦,常立萍,等.潜阳解毒通络饮通过PPAR-γ途径抑制炎症因子表达从而对自发性高血压大鼠炎症机制干预的研究[J].环球中医药,2013,6(06):407-0.
　JIN Qu,DENG Yue,CHANG Li ping..Qianyang Jiedu Tongluo Decoction on inflammatory intervention mechanism in spontaneously hypertensive rats expression by acting on PPAR γ pathway inhibition of inflammatory factors[J].,2013,6(06):407-0.
点击复制

潜阳解毒通络饮通过PPAR-γ途径抑制炎症因子表达从而对自发性高血压大鼠炎症机制干预的研究()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年06期
页码:
407-0
栏目:
论著
出版日期:
2013-06-06

文章信息/Info

Title:
Qianyang Jiedu Tongluo Decoction on inflammatory intervention mechanism in spontaneously hypertensive rats expression by acting on PPAR γ pathway inhibition of inflammatory factors
作者:
靳取;邓悦;常立萍;
Author(s):
JIN Qu;  DENG Yue;  CHANG Liping.
Changchun University of Traditional Chinese Medicine Graduate School, Changchun 130000, China
关键词:
高血压病; 果蝇受体4; 过氧化物酶体增殖物激活受体γ; 潜阳解毒通络饮; 炎症因子
Keywords:
Hypertension; TLR4; PPARγ; Qianyang Jiedu Tongluo Decoction; Inflammatory factor
分类号:
R285
DOI:
-
文献标志码:
A
摘要:
目的探讨潜阳解毒通络饮治疗原发性高血压大鼠的作用机制。方法选择健康雄性12周龄原发性高血压大鼠(spontaneouslyhypertensiverats,SHR)71只,随机分为潜阳组、解毒组、通络组、全方组、缬沙坦组、吡格列酮组、SHR空白对照组,魏-凯二氏大鼠(Wistar-Kyotorats,WKY)大鼠10只作为正常血压对照组。给药2个月后处死取腹主动脉血和心脏左心室。用ELISA试剂盒测血清中超敏C反应蛋白(high-sensitivityc-reactiveprotein,hs-CRP)、肿瘤坏死因子(tumornecrosisfactor-a,TNF-a)、白介素1β(interleukin-lβ,IL-1β)的浓度。聚合酶链反应(polymerasechainreaction,PCR)测过氧化物酶体增殖物激活受体γ(peroxisomeproliferatorsactivatedreceptors,PPAR-γ)、果蝇受体4(toll-likereceptor4,TLR4)及TNF-a的mRNA在大鼠心肌细胞中的表达。结果潜阳解毒通络饮能够有效抑制SHR血清中IL-1β、TNF-α及hs-CRP炎症因子的表达,有统计学意义(P<0.05)。在SHR心肌组织mRNA的表达上,TNF-a、TLR4及PPAR-γ的mRNA表达与其他治疗组和对照组相比有统计学意义(P<0.05)。结论证实了潜阳解毒通络饮从"风痰瘀络"病机的角度治疗原发性高血压大鼠有效性,其作用机制可能与作用于PPAR-γ途径、改善炎症因子表达有关。
Abstract:
ObjectiveTo investigate the mechanism of Qianyang Jiedu Tongluo Decoction for the treatment of hypertensive rats. Methodshealthy male 12 weeks Lingyuan hypertension rat (SHR) 72, 10 WKY rats, randomly divided into Qianyang Group, detoxification group, Tongluo group, the whole Party group, valsartan group, SHR treatment group, blank control group and Control group. After 2 months of administration take blood from the abdominal aorta and the left ventricle of the heart. The concentration of serum hsCRP, TNFa, IL1 β ELISA kit. Expression of PPARγ, TNFa and TLR4 RTPCR mRNA in myocardial cells of rats. ResultsQianyang Jiedu Tongluo Decoction can effectively inhibit the expression of SHR in rat serum IL1β, TNFα and hsCRP inflammatory factors, with statistical significance (P＜005). The expression of mRNA in myocardial tissue of SHR rats on the TNFa, TLR4, and PPARγ mRNA expression compared with other treatment group and the control group was statistically significant (P＜005). Conclusionthe treatment of confirmed hypertensive rats from the “wind phlegm blood stasis collaterals” pathogenesis angle Qianyang Jiedu Tongluo Decoction is effective, its mechanism may be related to effects on PPAR γ way, improve the expression of inflammatory factors relat.

参考文献/References:

［1］BautistaLE, VeraLM, Arenas IA etal. Inde Pendentassoeiation between inflammatory markers(C Reactive Protein,interleukin6,and TNF alpha) and essential hypertension ［J］. JHum Hypertens,2005,19(2):149151.
［2］Parker LC, PrinceLR, SabroeI. TranslationalMinireviewserieson tolllike receptors: networks regulatedby tolllikereceptors mediate innate and adaptive immunity［J］. Clin Exp Immunol,2007,147(2):199207.
［3］Akira S, Uematsu S, Takeuchi O. Pathogen recognition and innateimmunity［J］. Cell， 2006, 124(4):783801.
［4］张莉莉, 李敬诚, 王景周,等. PPARγ在高血压血管平滑肌细胞表型转化中的作用［J］.第三军医大学学报，2009,31(4)311314.
［5］孙慧英,李涛. 脑卒中及其危险因素分析［J］. 卒中与神经疾病,2012,19(3)：167169.
［6］KimKI, LeeJH, Chang HJ, et al. Association between blood pressure variabilityand inflammatory marker in hypertensive patients［J］. Circulation, 2008, 72(2): 293298.
［7］Edfeldt K, Swedenborg J, Hansson GK,et al. Expression of tolllike receptor in human atherosclerotic ledions:a possible pathway for plaque activation［J］. Circulation,2002,105(10):11581161.
［8］Sheu JJ, Chang LT. Prognostic value of activated tolllike receptoer4 in monocytes following acute myocardial infarction［J］, 2007,154(3):581588.
［9］Hall G,Hasday JD,Rogers TB.Regulating the regulator:NFkappaBsignaling in heart［J］. J Mol Cell Cardiol,2006,41(4):580591.
［10］邓悦，常立萍，齐锋，等. 高血压从“风痰瘀络“论治的思考［J］. 中华中医药学刊,2011,29(4):750751.
［11］邓悦. 高血压“从毒论治”的思考［C］. 中华中医药学会第九届学术年会论文集,郑州,2007:237242.
［12］郭宇光.邓悦. 滋阴潜阳解毒通络饮对SHR左心室肥厚及心肌Ⅰ、Ⅲ型胶原的影响［J］.吉林中医药，2008.28(2)：145146.

相似文献/References:

[1]温桂荣.应用经方治疗高血压病经验浅谈[J].环球中医药,2013,6(03):201.
[2]吴爱明,赵明镜,张冬梅,等.高血压病患者血清白介素6对缺血性中风诊断和辨证的临床意义探讨[J].环球中医药,2015,8(04):399.[doi:10.3969/j.issn.1674-1749.2015.04.004]
　WU Ai-ming,ZHAO Ming-jing,ZHANG Dong-mei,et al.Discussion on the clinical value of serum interleukin- 6 for the diagnosis and syndrome differentiation of ischemic stroke in patients with hypertension[J].,2015,8(06):399.[doi:10.3969/j.issn.1674-1749.2015.04.004]
[3]陈仁波,张明明,李鹤,等.基于社区高血压病肝火亢盛证脉诊特征的客观化探索研究[J].环球中医药,2017,10(12):1443.[doi:10.3969/j.issn.1674-1749.2017.12.006]
　CHEN Renbo,ZHANG Mingming,LI He,et al.Objective exploration of pulse diagnosis characteristics about overabundant liver-fire syndrome based on community hypertension[J].,2017,10(06):1443.[doi:10.3969/j.issn.1674-1749.2017.12.006]

备注/Memo

备注/Memo:
基金项目：吉林省科技厅：熄风解毒通络饮干预高血压心肌肥厚微炎症信号转导及机制研究资助(201115177)
作者单位：130000 长春中医药大学研究生学院［靳取(硕士研究生)］；长春中医药大学附属医院心脏内科(邓悦、常立萍)
作者简介：靳取(1986 )，2010级在读硕士研究生。研究方向：高血压病。Email: jin79002316@163.com
通讯作者：邓悦(1962 )，博士，教授，博士生导师。研究方向：心血管研究与防治。Email: dyue7138@sina.com
更新日期/Last Update: 1900-01-01