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[1]郝钰,唐莹,王顺梅,等.十全大补汤调整肉桂剂量对氧化损伤大鼠真皮成纤维细胞增殖的影响[J].环球中医药,2013,6(09):648-0.
　HAO Jue,TANG Ying,WANG Shun mei,et al.Influence of Shiquan Dabu Decoction and different doses of cinnamon compatibility on oxidativedamaged rat dermal fibroblasts proliferation[J].,2013,6(09):648-0.
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十全大补汤调整肉桂剂量对氧化损伤大鼠真皮成纤维细胞增殖的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年09期
页码:
648-0
栏目:
论著
出版日期:
2013-09-06

文章信息/Info

Title:
Influence of Shiquan Dabu Decoction and different doses of cinnamon compatibility on oxidativedamaged rat dermal fibroblasts proliferation
作者:
郝钰;唐莹;王顺梅;杜贯潮;代红雨;
Author(s):
HAO Jue;  TANG Ying;  WANG Shunmei;  et al.
School of Preclinical Medicine Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
氧化应激; 创面修复; 纤维细胞细胞; 十全大补汤; 肉桂
Keywords:
Oxidative stress; Wound healing; Dermal fibroblasts; Shiquan Dabu Decoction
分类号:
R2855
DOI:
-
文献标志码:
A
摘要:
目的从细胞分裂周期变化角度探讨十全大补汤调整肉桂剂量促进创面修复及皮肤修复的机理。方法采用体外培养成纤维细胞,造成氧化损伤成纤维细胞模型。以血清药理学方法获取大鼠血清,用以对成纤维细胞模型的干预。并运用四甲基偶氮唑盐微量酶反应比色法(MMT法)及流式细胞仪检测成纤维细胞增殖及分裂周期的变化。结果MMT法结果显示2倍肉桂组和4倍肉桂组对氧化损伤的大鼠真皮成纤维细胞具有促进增殖的作用,与模型组比较差异显著(P<0.01);流式细胞术结果显示经500μmol/L的H2O2刺激30分钟的大鼠真皮成纤维细胞中G0/G1期细胞比例显著增高,S期和G2/M期细胞比例显著降低。在药物干预24小时后,四个用药组均可降低G0/G1期细胞比例,S期和G2/M期细胞比例升高;在药物干预48小时后,除了去肉桂组以外,其余三个用药组均能使G0/G1期细胞比例降低,S期和G2/M期细胞比例升高,与模型组相比差异具有统计学意义(P<0.05),而去肉桂组对细胞周期的影响与模型组相比无明显差异。结论十全大补汤对氧化损伤的大鼠真皮成纤维细胞具有调控成纤维细胞周期、促进增殖的作用,而适当增加肉桂剂量可以起到增效的作用。
Abstract:
ObjectiveTo investigate the mechanism of Shiquan Dabu Decoction (literally, Complete Tenmedicinals Nourishment Decoction) with varying doses of cinnamon composed in its benefits to wound/skin repair from the perspective of cell division and cell cycle. MethodsRat dermal fibroblasts was obtained by invitro culture. The serums extracted with serum pharmacological approaches were used to interfere the oxidativedamaged rat dermal fibroblasts. Next, Methyl thiazol tetrazolium (MTT) assay was used to measure the effects of the serums on cell proliferation; and flow cytometry were employed to test the effects on cell cycle. ResultsOD value decreased significantly in MTTassay when 500 μmol/L H2O2 had stimulated rat dermal fibroblasts for 30 minutes. And the proportion of G0/G1 period increased significantly while the proportion of S and G2/M period decreased significantly. The rate of apoptosis increased significantly. Mitochondrial membrane potential decreased significantly. And the level of reactive oxygen species increased significantly. After 24 hours of serums treatment, the OD values of the double cinnamon group and the four times cinnamon group increased in the group of 10% serum concentration. The OD value of the double cinnamon group increased significantly in the group of 15% serum concentration. After 48 hours of serums treatment, the OD values of the double cinnamon group and the four times cinnamon group increased significantly in the group of 5% serum concentration. The OD value of the double cinnamon group increased significantly in the group of 10% serum concentration. The OD values of the double cinnamon group in two time points were both the highest in the four groups. After 24 hours of serums treatment, the proportion of G0/G1 period of all four groups decreased significantly, and the proportion of S and G2/M period of all four groups increased significantly. After 48 hours of serums treatment, the proportion of G0/G1 period of the Shiquan Dabu Decoction group, the double cinnamon group and the four times cinnamon group decreased, and the proportion of S and G2/M period of the three groups increased. The proliferation index of the double cinnamon group in two timepoints were both the highest in the four groups. Conculusion Shiquan Dabu Decoction can accelerate wound healing into skin restoration through promoting cell proliferation controlling the proliferation of filbrohlasts from granulation tissue of the wound. And the function can be enhanced if the dose of cinnamon is increased on the basis of Shiquan Dabu Decoction.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：北京市自然科学基金(7112072)；北京中医药大学自主选题资助项目(20111003)
作者单位：100029 北京中医药大学基础医学院［郝钰、唐莹(硕士研究生)、王顺梅(硕士研究生)］；北京中医药大学东方医院肛肠科［代红雨、杜冠潮(硕士研究生)］
作者简介：郝钰(1964- )，女，博士，教授。研究方向：中药的免疫调节机制研究。Email: huangshiren64@qq.com
通讯作者：代红雨(1971- )，博士，副主任医师。研究方向：中医药促进难愈性创面愈合的研究。Email: dhy515@163.com
更新日期/Last Update: 1900-01-01