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[1]李小琴,高玉峰,于跃,等.电针对大鼠脊髓中抑制性生长因子的影响[J].环球中医药,2013,6(09):653-0.
　LI Xiao qin,GAO Yu feng,YU Yue,et al.Investigation of electro acupuncture therapy on inhibitory growth factor in rat spinal cord[J].,2013,6(09):653-0.
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电针对大鼠脊髓中抑制性生长因子的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年09期
页码:
653-0
栏目:
论著
出版日期:
2013-09-06

文章信息/Info

Title:
Investigation of electro acupuncture therapy on inhibitory growth factor in rat spinal cord
作者:
李小琴;高玉峰;于跃;耿楠;于天源;
Author(s):
LI Xiaoqin;  GAO Yufeng;  YU Yue;  et al.
School of Acupuncturemoxibustion and tuina, Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
电针; 坐骨神经损伤; 髓鞘相关糖蛋白(MAG)
Keywords:
Electro acupuncture; Sciatic nerve injury; Myelin associated glycoprotein (MAG)
分类号:
R245
DOI:
-
文献标志码:
A
摘要:
目的通过电针对坐骨神经损伤大鼠脊髓中髓鞘相关糖蛋白(myelinassociatedglycoprotein,MAG)表达影响的实验研究,进一步探讨电针治疗周围神经损伤的修复机理,为临床治疗提供理论支持。方法采用神经夹持损伤的方式建立大鼠坐骨神经损伤模型,通过坐骨神经功能指数(sciaticnervefunctionindex,SFI)及热痛阈检测观察大鼠的行为学变化;通过免疫组织化学染色观察大鼠脊髓内MAG表达情况,进而统计分析各组间的差异。结果行为学检测结果显示,在基本功能改善方面,与模型组及模型对照组比较,电针治疗组(P<0.05)大鼠右后腿感觉及运动功能明显改善。免疫组化结果显示模型组、模型对照组及电针组MAG表达均比正常组高(P<0.05),但电针组治疗1、2个疗程后,MAG表达明显降低,越来越接近正常组。结论通过本实验研究发现,电针能改善坐骨神经损伤大鼠的一般状况,电针组大鼠脊髓中MAG的表达明显降低,说明电针能抑制脊髓中抑制性生长因子的释放,促进周围神经损伤再生修复。
Abstract:
ObjectiveThrough the experimental study on the effect of MAG expression in the rat spinal cord of electro acupuncture on sciatic nerve injury, to further explore the repair mechanism of electro acupuncture for treatment of peripheral nerve injury, to provide theoretical support for clinical treatment. MethodUse the nerve clamping method to make the sciatic nerve injury model, through the sciatic nerve function index (SFI) and heat pain threshold detection observation rat behavior change; by immunohistochemical staining were observed in the rat spinal cord within the MAG expression, and then statistical analysis of the differences between groups. ResultsBehavioral testing results, improvements in basic function, compared with the model control group and model group, electro acupuncture group (P＜005) of sensory and motor function of rat right hind improve. The results of immunohistochemistry showed that in model group, model group and electro acupuncture group MAG expression were higher than that of the normal group (P＜005), but the acupuncture treatment group 1 or 2 months after treatment, the expression of MAG was significantly decreased, more and more close to the normal group. ConclusionThe study found that, the general situation of electro acupuncture can improve the rat sciatic nerve, spinal cord of rats in the electro acupunctue group the expression of MAG decreased remarkably, indicating that electro acupuncture can inhibit the release of growth factor inhibition in the spinal cord, promote the regeneration of peripheral nerve injury.

参考文献/References:

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［5］Wong EV, David S, Jacob MH, et al. Inactivation of myelinassoiciated glycoprotein enhancesoptic nerve regeneration［J］. J Neurosci,2003,23(8):31123117.
［6］McKerracher L, David S, Jackson DL, et al. Identification of myelinassociated glycoprotein as amajormyelinderived inhibitor of neurite growth［J］. Neuron,1994,13(4):805811.
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备注/Memo

备注/Memo:
基金项目：国家自然科学基金(30873311)
作者单位：100029 北京中医药大学针灸推拿学院［李小琴(硕士研究生)、于跃(硕士研究生)、耿楠(硕士研究生)、高玉峰(博士研究生)、于天源］
作者简介：李小琴(1986- )，女，2011级在读硕士研究生。研究方向：针灸推拿治疗周围神经损伤的机理研究。Email: lixiaoqin041009@163.com
通讯作者：于天源(1965- )，博士，教授，主任医师。研究方向：针灸推拿治疗周围神经损伤的机理研究。Email: yutianyuan@sina.com
更新日期/Last Update: 1900-01-01