|本期目录/Table of Contents|

[1]陈惠,辛丽丽,龚婕宁,等.基于全转录组测序技术的转录组学在中医药领域的应用前景分析[J].环球中医药,2013,6(10):759-0.
　CHEN Hui,XIN Li li,GONG Jie ning..Application prospect analysis in the field of traditional Chinese medicine based on the transcription of RNAseq technology[J].,2013,6(10):759-0.
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基于全转录组测序技术的转录组学在中医药领域的应用前景分析()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年10期
页码:
759-0
栏目:
综述
出版日期:
2013-10-06

文章信息/Info

Title:
Application prospect analysis in the field of traditional Chinese medicine based on the transcription of RNAseq technology
作者:
陈惠;辛丽丽;龚婕宁;
Author(s):
CHEN Hui;  XIN Lili;  GONG Jiening.
College of Basic Medicine, Nanjing University of Chinese Medicine. Nanjing 210046, China.
关键词:
中医药研究; 分子生物学; 转录组学; 全转录组测序技术
Keywords:
Traditional Chinese medicine research; Molecular biology; Transcriptomics; RNAseq
分类号:
R34
DOI:
-
文献标志码:
A
摘要:
转录组学以其在全基因组转录水平上整体性、系统性的研究特点在中医药研究中发挥了重要作用,近年来兴起的全转录组测序(RNASequencing,RNA-Seq)技术使转录组学研究进入了数字化、信息化的时代。本文总结归纳了RNA-seq技术在转录组学中的优势,其整体性、系统性、个体组织差异性、时间独立性等优势改善了以往单基因研究不能切合中医学整体观念的不足;着重探讨了基于RNA-seq技术的转录组学在中医学理论及方药研究等方面的应用现状及前景,对其在中医辨治理论实质研究、方药有效成分的筛选和作用机制研究及临床用药安全评定体系等方面的应用策略进行了分析,并提出了初步的实验方案。
Abstract:
For the feature of integrity and systematicness on the level of the whole genome, the transcriptomics play a significant role in research of Chinese medicine, the recently arisen RNA deep sequencing technology(RNAseq) allows for the age of digital and information. In this paper, we summarize the advantages of RNAseq in the transcriptomics, its integrity, systematicness, individual tissue differences, time independence and many other edges have solved the previous research problem that single gene research cant failed to correspond with the perspective of holism in traditional Chinese medicine (TCM). This paper discusses the transcriptome study based on the technology of RNAseq in TCM theory and the formulas and medicine about their present situation in application and prospect. Analysis has been made on the application strategy in the research of syndrome differentiation in TCM, the selection of active ingredients of formulas and medicine and the research of mechanism, clinical drug safety evaluation system, and a preliminary experimental scheme was proposed.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81373603）
作者单位： 210046 南京中医药大学基础医学院温病教研室［陈惠（硕士研究生）、辛丽丽（硕士研究生）、龚婕宁］
作者简介： 陈惠（1989- ），女，2012级在读硕士研究生。研究方向：中医临床基础。Email：1060138599@qq.com
通讯作者：龚婕宁（1957- ），女，博士，博士生导师。研究方向：温病治则治法研究。Email:13901580022@163.com
更新日期/Last Update: 1900-01-01