|本期目录/Table of Contents|

[1]宋海龙 朱国强 卢晓丽等.假龙胆总多酚含量测定及抗氧化 活性研究[J].环球中医药,2014,7(02):103-0.
 SONG Hai long,ZHU Guo qiang,LU Xiao li,et al.Gentianella turkestanorum (Gand.) Holub. total polyphenol content and antioxidant activity determination[J].,2014,7(02):103-0.
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假龙胆总多酚含量测定及抗氧化 活性研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年02期
页码:
103-0
栏目:
论著
出版日期:
2014-02-06

文章信息/Info

Title:
Gentianella turkestanorum (Gand.) Holub. total polyphenol content and antioxidant activity determination
作者:
宋海龙 朱国强 卢晓丽等
Author(s):
SONG HailongZHU GuoqiangLU Xiaoliet al.
Xinjiang Institute of Chinese Materia Medica and Ethical Materia Medica,Urumqi 830002,China
关键词:
假龙胆总多酚抗氧化
Keywords:
Gentianella turkestanorum(Gand.) Holub.Total PolyphenolAntioxidant activity
分类号:
R284.2
DOI:
-
文献标志码:
A
摘要:
目的建立假龙胆总多酚含量的测定方法。 方法采用可见分光光度法测定假龙胆中总多酚的含量,并建立清除羟基自由基和超氧阴离子自由基两种抗氧化模型对假龙胆抗氧化能力进行评价。结果以没食子酸作为测定总多酚含量的对照品,在浓度005~03 mg (r=09999)范围内呈良好的线性关系,结果表明,总多酚含量平均值为763 mg,假龙胆提取液具有良好的抗氧化能力。结论此方法操作简便,重现性良好、稳定,可作为假龙胆总多酚的检测方法,并可作为天然抗氧化剂资源进行开发研究。
Abstract:
ObjectiveTo establish a method for the determination of total Polyphenol in Gentianella turkestanorum (Gand.) Holub. MethodsThe total Polypheno land in Gentianaceae was determined by visible spectrophotometry,and establish two kinds of antioxidation modelscavenging hydroxyl radicals and scavenging superoxide anion radicalsto evaluate Gentianella turkestanorum (Gand.) Holub. in its antioxidant ability. ResultsThe method had a good linearity in the range of 005~03 mg(r=09999)with gallic acid as the reference substance. The results showed that an average total polyphenol content of 763 mg, Gentianella turkestanorum (Gand.) Holub. extracts had good oxidation resistance. ConclusionsThis method was simple,with good reproducibility,and stability, and could be adopted as the standard method for detecting Gentianella turkestanorum (Gand.) Holub. total polyphenols. Gentianella turkestanorum (Gand.) Holub , as a natural antioxidant resources, is worth further research and development.

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔药与特色天然药物创制及产业化示范项目(2011301051);重大公共卫生专项——全国第四次中药资源普查新疆(试点)项目(2100409)
作者单位:830001 乌鲁木齐,新疆中药民族药研究所科教科[宋海龙(硕士研究生)、朱国强、贾晓光)];新疆医科大学中医学院中药系[卢晓丽(硕士研究生)、田树革)]作者简介:宋海龙(1973- ),2102级在职硕士研究生,实验师。研究方向:食品加工与安全。Email:xjwssong@126.com通讯作者:贾晓光(1955- ),硕士,主任药师,硕士生导师。研究方向:中药资源与化学。Email:xgjia@vip.sina.com.cn
更新日期/Last Update: 1900-01-01