|本期目录/Table of Contents|

[1]郭云霞 李绍旦 刘毅 杨明会.补肾活血颗粒对帕金森大鼠脑腺苷A2A受体 影响的研究[J].环球中医药,2014,7(03):168-0.
　GUO Yun xia,LI Shao dan,LIU Yi,et al.Effect of Bushen Huoxue Granule(BSHXG) on adenosine A2A receptor in brain of Parkinsons disease(PD) rats[J].,2014,7(03):168-0.
点击复制

补肾活血颗粒对帕金森大鼠脑腺苷A2A受体 影响的研究()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年03期
页码:
168-0
栏目:
论著
出版日期:
2014-03-06

文章信息/Info

Title:
Effect of Bushen Huoxue Granule(BSHXG) on adenosine A2A receptor in brain of Parkinsons disease(PD) rats
作者:
郭云霞 李绍旦 刘毅 杨明会
Author(s):
GUO Yunxia;  LI Shaodan ; LIU Yi;  et al.
Department of Traditional Chinese Medicine, PLA General Hospital, Beijing 100853, China
关键词:
补肾活血颗粒; 帕金森模型大鼠; 腺苷A2A受体
Keywords:
Bushen Huoxue Granule(BSHXG); Parkinsons disease rats; Adenosine A2A receptor
分类号:
R7425
DOI:
-
文献标志码:
A
摘要:
目的探讨补肾活血颗粒治疗帕金森病的作用机制。方法用6羟基多巴损毁脑右侧内侧前脑束的方法建立帕金森大鼠模型。将33只SD模型大鼠随机分为治疗组18只和对照组15只，另设正常组15只，治疗组以中药补肾活血颗粒灌胃每日1次，连续8周。对照组及正常组同法以生理盐水灌胃8周，灌胃结束、观察帕金森大鼠旋转行为变化，后断头取脑，免疫荧光法观察大鼠脑腺苷A2A受体变化。结果治疗组大鼠旋转行为较对照组改善，治疗组大鼠脑纹状体A2A受体表达较对照组明显减弱（P＜0.01），模型组比正常组表达显著增强（P＜0.01）。三组大鼠黑质中A2A受体均无表达。结论补肾活血颗粒能改善帕金森大鼠旋转行为，抑制大鼠纹状体腺苷A2A受体表达，对帕金森病治疗起到较好疗效。
Abstract:
ObjectiveTo explore the mechanism of treating Parkinsons disease(PD)with Bushen Huoxue Granule(BSHXG). MethodsEstablishing rat model of PD with 6hydroxydopamine lesion as medial forebrain bundle the right side of the brain of. 33 PD rats modes were randomly divided into treatment group 18 (BSHXG group) and control group 15. Additionally other 15 SD rats were selected in normal group. BSHXG was applied for treatment group once daily for 8 weeks. Physiologic saline was applied for control group and normal group in the same way. Gavage end, observed changes in the rotational behavior of PD rats. After decapitated, Immunofluorescence was used to observe the changes of adenosine A2A receptor in rat brain. ResultsRotational behavior of rats in the treatment group was much better improved than in the control group.A2A receptor expression in the treatment group compared with the control group was significantly decreased(P<001).A2A receptor expression in the model group was significantly increased than the normal group(P<001). A2A receptor were not expression in three groups of rats substantia nigra. ConclusionBSHXG can improve the rotational behavior of PD rats, inhibit adenosine A2A receptor expression in PD rat striatum, and has a significant therapeutic effect on the treatment of Parkinsons disease.

参考文献/References:

[1]Russell WMS, Burch RL. The Principles of Humane Experimental Technique［ M］ . London: Methuen, 1959.
[2］包新民,舒斯云.大鼠脑立体定位图谱［M］.北京：人民卫生出版社，1991：4959．
[3］施新猷．医学动物实验方法［M］.北京：人民卫生出版社,1986：11．
[4］ChuYY, TuKH, Lee YC, et al .Characteri zation of the ratA2a adenosine receptor gene . DNA Cell ［J］. Bio,1996,15(4):329337.
[5］Svenningsson P，Hall H，Sedvai1 G，et al．Distribution of adenosine receptors in the postmortem human brain：an extended autoradiographic study［J］．Synapse，1997，27(4)：322335．
[6］Fink JS,Weaver DR， Rivkees SA， et al.Molecular Cloning of the rat A2 adenosine recepoter :Selective co-expression with D2 dopamine receptors in rat striatum［J］. Brain Res Mol Brain Res,1992，14:186195
[7］Svenningsson P, Le Moine C,Kull B， et al.Cellular expression of adenosine A2A recepoter messenger RNA in the rat central nervous system with special reference to dopamine inervted areas［J］. Neuroscience,1997，80:11711185
[8］Fuxe K ,Ferre S,Canals M， et al. Adenosine A2A and dopamine D2 heteromeric receptor complexes and their function［J］. Journal of Molecular Neuroscience,2005，26(23):209220.
[9］Pinna A，Wardas J ，Simola N，et al．New therapies for the treatment of Parkinsons disease：Adenosine A2A receptor antagonists ［J］．Life Sci，2005，77(26)：32593267．

相似文献/References:

备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81273969）
作者单位：100853 北京，解放军医学院［郭云霞（博士研究生）］；解放军总医院中医科（郭云霞、李绍旦、刘毅、杨明会）作者简介：郭云霞（1976 ），女，2011级在读博士研究生。研究方向：中西医结合老年病。Email: guoyx1999@sina.com通讯作者：杨明会（1962 ），博士，教授，主任医师，博士生导师。中华中医药学会副会长，中国人民解放军中医药学会会长。研究方向：老年病中医研究。Email: ymh9651@yahoo.com.cn
更新日期/Last Update: 1900-01-01