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[1]杨悦 孔祥英 万红叶 苏晓慧 林娜.地乌三萜皂苷W1对破骨细胞分化和骨吸收功能 的影响[J].环球中医药,2014,7(03):191-0.
　YANG Yue,KONG Xiang ying,WAN Hong ye,et al.Effect of triterpene saponin W1 from rhizome of Anemone Flaccid on osteoclasts differentiation and bone resoroption[J].,2014,7(03):191-0.
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地乌三萜皂苷W1对破骨细胞分化和骨吸收功能 的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年03期
页码:
191-0
栏目:
论著
出版日期:
2014-03-06

文章信息/Info

Title:
Effect of triterpene saponin W1 from rhizome of Anemone Flaccid on osteoclasts differentiation and bone resoroption
作者:
杨悦 孔祥英 万红叶 苏晓慧 林娜
Author(s):
YANG Yue;  KONG Xiangying;  WAN Hongye; et al.
Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
关键词:
地乌三萜皂苷W1; 小鼠单核/巨噬细胞RAW2647; 肿瘤坏死因子α; 骨吸收
Keywords:
Triterpene saponin W1 from rhizome of Anemone Flaccid ; RAW2647; Tumor necrosis factorα; Bone resoroption
分类号:
R2855
DOI:
-
文献标志码:
A
摘要:
目的探讨地乌三萜皂苷类成分W1对体外破骨细胞分化及骨吸收功能的影响。方法3个不同浓度(00625，0125，025 μg/ml)的W1与核因子κB受体活化因子配体(receptor activator of nuclear factor kB ligand，RANKL)诱导的小鼠单核/巨噬细胞RAW 2647共孵育7天，抗酒石酸酸性磷酸酶（tartrate resistant acid phosphatase，TRAP）染色观察TRAP阳性多核细胞的形成，甲苯胺蓝染色及扫描电镜观察骨吸收陷窝的形成，图像分析计算骨吸收陷窝面积百分比；同3个浓度的W1与RANKL诱导的RAW2647细胞共孵育24小时后收集细胞上清液，放射免疫法检测肿瘤坏死因子α（tumor necrosis factorα，TNFα）的含量；甲基噻唑基四唑（methyl thiazolyl tetrazolium，MTT）法检测W1对RAW2647细胞毒性影响。结果RANKL组诱导RAW 2647细胞形成多量TRAP阳性多核细胞，形成明显的骨吸收陷窝，并诱导TNFα在RAW2647细胞上清中异常高表达；与RANKL组相比，3个浓度的W1均能显著减少TRAP阳性细胞数(P＜001)，显著减少骨吸收陷窝面积(P＜001，P＜0001，P＜0001)，明显降低TNFα的表达量（P＜005，P＜001，P＜001），且未观察到对RAW2647的细胞毒性。结论W1对由RANKL诱导的体外破骨细胞分化和骨吸收功能有一定的负调节作用。
Abstract:
ObjectiveTo observe the effect of W1 from rhizome of Anemone Flaccid on osteoclasts formation and bone resoroption in vitro. MethodsRAW2647 cells induced by RANKL were cultured with three variable concentration(00625,0125,0.25 μg·ml-1 )of W1 in vitro for 7 days. The influence of W1 on osteoclasts differentiation was assessed by osteoclastlike number and tartrate resistant acid phosphatase (TRAP) activity; resorption lacunae were stained by toluidine blue and were visualized by Philips XL30 scanning electron microscope. The number of TRAP+ cells and the percentage of resorption pits were counted. RAW2647 cells induced by RANKL were incubated with three variable concentration of W1 in vitro for 24 hours, the level of TNFα in cell culture supernatant was examined by Iodine\
[125I\] Tunmor Necrosis Factor Radioimmunoassay kit; RAW2647 cell viability was assayed by MTT. ResultsRANKL could induce RAW2647 cells to form TRAP positive multinucleated cells and form bone resorption pits on bone. RANKL could induce abnormally high expression of TNFα in RAW2647 cells. W1 (00625,0125,025 μg· ml-1 )could decrease the number of TRAP+ cells (P＜001) as well as the area of bone resorption pits(P＜001，P＜0001，P＜0001),and showed no cytotoxicity to RAW2647; W1 (00625025 μg· ml-1 )could suppresse the expression of TNFa（P＜005，P＜001，P＜001）. ConclusionRANKL induced osteoclastogenesis in these monocytic cells,and W1 inhibited RANKLinduced tumor necrosis factorα production and osteoclast differentiation and bone resorption pit formation.

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备注/Memo

备注/Memo:
基金项目：国家科技重大专项(2011ZX0910100603)；北京市自然科学基金(7112096)；中国中医科学院基本科研业务费自主选题（ZZ070836）
作者单位：100700 北京，中国中医科学院中药研究所中药理论与本草文献研究中心作者简介：杨悦(1989- )，女， 2011级硕士研究生。研究方向：中药药理研究。Email: yangyue8675658@163.com通讯作者：林娜(1963- )，女，博士，研究员，博士生导师。研究方向：中药药性理论与中药药理。Email:linna888@163.com
更新日期/Last Update: 1900-01-01