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[1]鄢泽然 张青 王潇慧等.柴贝止痫汤影响大鼠脑微血管内皮细胞乳腺癌耐药蛋白、核因子 p65 表达的研究[J].环球中医药,2014,7(04):241-0.
　YAN Ze ran,ZHANG Qing,WANG Xiao hui,et al.Role of Chaibei Zhixian Decoction on regulating the expression of BCRP and NFκB p65 in rat brain microvascular endothelial cells[J].,2014,7(04):241-0.
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柴贝止痫汤影响大鼠脑微血管内皮细胞乳腺癌耐药蛋白、核因子 p65 表达的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年04期
页码:
241-0
栏目:
论著
出版日期:
2014-04-06

文章信息/Info

Title:
Role of Chaibei Zhixian Decoction on regulating the expression of BCRP and NFκB p65 in rat brain microvascular endothelial cells
作者:
鄢泽然 张青 王潇慧等
Author(s):
YAN Zeran;  ZHANG Qing;  WANG Xiaohui;  et al.
Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
大鼠脑微血管内皮细胞:7156; 乳腺癌耐药蛋白:5068; 止痫汤:4052; 核因子:2344; 表达:936; 研究:539
Keywords:
Chaibei Zhixian Decoction;  Breast cancer resistance protein (BCRP/ABCG2);  Rat brain microvascular endothelial cell;  Intractable epilepsy
分类号:
R2777
DOI:
-
文献标志码:
A
摘要:
目的探索中药柴贝止痫汤对离体大鼠脑微血管内皮细胞乳腺癌耐药蛋白（breast cancer resistance protein, BCRP）和核因子κB（nuclear factorkappa B, NFκB）p65表达的影响。方法培养原代大鼠脑微血管内皮细胞，传至3代时分为正常组、模型组和中药组，正常组常规培养，模型组用100 nmol/L的地塞米松作用细胞24小时，诱导细胞膜上BCRP表达增加；中药组在造模基础上分别用柴贝止痫汤四个浓度（10 μg/ml、100 μg/ml、500 μg/ml、1 mg/ml）干预模型细胞24小时。Western blot检测各组细胞BCRP、NFκB p65的表达，差异比较采用方差分析；Realtime PCR检测Bcrp mRNA的扩增，相对定量法比较各组间扩增差异。结果柴贝止痫汤10 μg/ml、100 μg/ml组BCRP表达及Bcrp mRNA的扩增均较模型组降低（P＜005）；柴贝止痫汤1 mg/ml组NFκB p65的表达较模型组降低（P＜005）。结论柴贝止痫汤可以下调地塞米松诱导的大鼠脑微血管内皮细胞BCRP及Bcrp mRNA的表达；可能对NFκB p65的表达有下调作用。
Abstract:
ObjectiveTo observe the effects of Chaibei Zhixian Decoction on regulating the expression of breast cancer resistance protein (BCRP) and nuclear factorkappaB (NFκB) p65 in rat brain microvascular endothelial cells. MethodsPrimary culture of brain microvascular endothelial cells were prepared from rats. The 3rd passage cells were exposed to dexamethasone (100 nmol/L) for 24h to upregulate the expression of BCRP. After dexamethasone administration, the cells were treated with increasing concentrations (10 μg/ml, 100 μg/ml, 500 μg/ml and 1 mg/ml) of Chaibei Zhixian Decoction for 24h. BCRP and NFκB p65 protein expression were assessed by Western blot analysis and data were measured with analysis of variance. Realtime PCR was performed for Bcrp mRNA amplification and differences among groups were assessed by relative quantitative assay. ResultsExposure to Chaibei Zhixian Decoction(10 μg/ml, 100 μg/ml)resulted in significant decrease in protein and mRNA levels of BCRP (P＜0.05); While BCRP was downregulated, NFκB p65 expression was decreased by Chaibei Zhixian Decoction(1 mg/ml) (P＜005). ConclusionChaibei Zhixian Decoction downregulate both protein and mRNA expression of BCRP and probably decrease the levels of NFκB p65 in rat brain microvascular endothelial cells.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81072771）；教育部博士学科点专项科研基金（20120013110011）；北京中医药大学科研创新团队资助项目（2011CXTD23）
作者单位：100078北京中医药大学东方医院脑病科［鄢泽然（博士研究生）、张青（博士研究生）、王潇慧（硕士研究生）、聂莉媛（博士研究生）、刘金民］；天津中医药大学第二附属医院急诊科（王越）
作者简介：鄢泽然（1985），2011级在读博士研究生。研究方向：中医药调节难治性癫痫耐药蛋白表达的作用及机制。Email: yanzeran@126.com
通讯作者：刘金民（1965），博士，教授，主任医师。研究方向：中医内科急症及发作性脑病的中医药治疗。Email: jmvip@vip.163.com
更新日期/Last Update: 1900-01-01