|本期目录/Table of Contents|

[1]陈建武 王晓娟.黄连碱对软骨细胞增殖及转化生长因子 β1mRNA 基因的表达的影响[J].环球中医药,2014,7(04):254-0.
　CHEN Jian wu,WANG Xiao juan..Effect of coptisine on proliferation of rat articular chondrocytes cultured in vitro and mRNA expression of transforming growth factorbeta 1[J].,2014,7(04):254-0.
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黄连碱对软骨细胞增殖及转化生长因子 β1mRNA 基因的表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年04期
页码:
254-0
栏目:
论著
出版日期:
2014-04-06

文章信息/Info

Title:
Effect of coptisine on proliferation of rat articular chondrocytes cultured in vitro and mRNA expression of transforming growth factorbeta 1
作者:
陈建武 王晓娟
Author(s):
CHEN Jianwu;  WANG Xiaojuan.
Medical Division of Xinjiang military region, Urumqi 830042, China
关键词:
软骨细胞增殖:6558; 转化生长因子:5847; 黄连碱:4231; 基因:1909; 表达:1112
Keywords:
Coptisine; Chondrocyte; Proliferation; Transforming growth factorβ1
分类号:
R2856
DOI:
-
文献标志码:
A
摘要:
目的观察黄连碱对Sprague Dawley大鼠关节软骨细胞体外增殖及转化生长因子β1mRNA基因表达的影响，探讨其治疗骨关节炎的作用及可能机制。方法取SD大鼠关节软骨，剪碎后，采用酶消化法原代分离培养软骨细胞，取处于对数生长期第3代细胞随机分组，实验组加入含10 μm黄连碱的培养基，对照组仅加入普通培养基。培养48小时后在倒置显微镜下观察软骨细胞形态，MTT法检测黄连碱在1，3，5，7天对软骨细胞增殖影响，实时荧光定量PCR分别检测培养7天后实验组和对照组细胞转化生长因子β1mRNA表达。结果培养48小时后实验组软骨细胞生长密集程度明显优于对照组；MTT检测也显示实验组细胞增殖情况优于对照组；培养7天后实验组转化生长因子β1mRNA表达显著高于对照组。结论黄连碱能促大鼠软骨细胞增殖，并提高转化生长因子β1mRNA的表达，提示这可能是其治疗骨关节炎的可能机制之一。
Abstract:
ObjectiveTo observe the effect of coptisine on the proliferation of rat articularchondrocyte and transforming growth factorβ1 mRNA expression, and then to explore the role and possible mechanism of coptisine in the treatment of osteoarthritis. MethodsKnee cartilage was shredded after harvested from SD rats under sterile conditions, and chondrocytes were isolated and cultured by the way of enzymatic digestion. After identifying by toluidine blue staining, the thirdpassage cells in the logarithmic growth phase were cultured in vitro and randomly divided into two groups after adherence. The experimental groups were cultured in DMEM with 10 μm coptisine, while the control group was given with normal medium alone. Chondrocytes morphology was observed under an microscope after 48 hours. 1, 3, 5, 7 days later, Methyl Thiazolyl Tetrazolium (MTT) assay method was adopted to observe the influence of coptisine on the proliferation of chondrocytes.Transforming growth factorβ1 mRNA levels were detected and analysed by quantitative real time polymerase chain reaction(qRTPCR) at 7days after cell culture.ResultsThe cell growth intensity in the coculture group was significantly better than in the control group observed under microscopeafter 48 hours after culture. MTT assay showed that cell proliferation in experimental group were higher than in the control group after 3 days of cell culture.transforming growth factorβ1 mRNA level in the coculture group weresignificantly better than the control group after 7 days of cell culture. Conclusioncoptisine can promote proliferation of chondrocyte and transforming growth factorβ1 mRNA expression, and these may be one of the possible mechanisms thatcoptisine can work in the treatment of osteoarthritis.

参考文献/References:

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备注/Memo

备注/Memo:

作者单位：830042 乌鲁木齐，新疆军区联勤部卫生处（陈建武）；解放军第23医院药械科（王晓娟）
作者简介：陈建武（1974 ），本科，主治医师。研究方向：卫生事业管理。Email: chenjianwu491@126.com
通讯作者：王晓娟(1972 )，女，本科，主管药师。研究方向：药理研究及临床应用。Email: wangxiaojuan491@126.com
更新日期/Last Update: 1900-01-01