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[1]赵轩,高彦彬,朱智耀,等.糖脂平对胰岛素抵抗大鼠骨骼肌氧化应激的影响[J].环球中医药,2014,7(05):333-337.
　ZHAO Xuan,GAO Yan bin,ZHU Zhi yao,et al.Effects of Tangzhiping on oxidative stress in rat insulin resistance model[J].,2014,7(05):333-337.
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糖脂平对胰岛素抵抗大鼠骨骼肌氧化应激的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年05期
页码:
333-337
栏目:
论著
出版日期:
2014-05-06

文章信息/Info

Title:
Effects of Tangzhiping on oxidative stress in rat insulin resistance model
作者:
赵轩;高彦彬;朱智耀;耿建国;张娜;周盛楠;李娇阳;
Author(s):
ZHAO Xuan;  GAO Yanbin;  ZHU Zhiyao; et al.
School of Traditional Chinese Medicine, Capital Medical University, Beijing　100069, China
关键词:
糖脂平; 胰岛素抵抗; 氧化应激; 骨骼肌
Keywords:
Tangzhiping; Insulin resistance; Oxidative stress; Skeletal muscle
分类号:
R2855
DOI:
-
文献标志码:
A
摘要:
目的建立喂养型胰岛素抵抗（insulin resistance, IR）大鼠模型，观察糖脂平对喂养型IR大鼠氧化应激的影响。方法选用体重在180~200 g的8周龄雄性SD大鼠60只，适应性喂养一周后，随机分为空白对照组15只和造模组45只。空白对照组采用普通饲料喂养，造模组采用高脂饲料喂养。8周造模成功后，将造模组随机分为：模型组、糖脂平组、罗格列酮组各15只。糖脂平组给予糖脂平按生药20 g/kg·d灌胃；罗格列酮组给予罗格列酮08 mg/kg·d灌胃；模型组和空白对照组灌服等量的生理盐水；末次给药后禁食12小时，用正常血糖胰岛素钳夹技术评价胰岛素敏感性，检测各组大鼠的体重、血糖、血清胰岛素水平，并留取骨骼肌，检测组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶 （GSHPx）、丙二醛（MDA）、过氧化氢酶（CAT）等指标。结果糖脂平能增加胰岛素敏感性，改善胰岛素抵抗，糖脂平组的M值显著高于模型组，体重、血糖、胰岛素均低于模型组，差异有统计学意义(P＜005)，与罗格列酮组比较，体重一项有统计学意义(P＜005)，血糖与胰岛素无统计学意义(P＞005)；糖脂平组SOD、GSHPx、CAT水平显著高于模型组,MDA显著低于模型组，差异有统计学意义(P＜005),与罗格列酮组比较，差异无统计学意义(P＞005)。结论糖脂平可改善胰岛素抵抗，也可改善氧化应激，反应了糖脂平可以通过改善胰岛素抵抗的上游通路——氧化应激来提高机体代谢活力，改善胰岛素抵抗，其作用机制可能是通过改善骨骼肌中线粒体的活性氧。
Abstract:
ObjectiveTo investigate the effects of Tangzhiping on oxidative stress in rats with insulin resistance. Methods60 male SD rats (weighs 180~200 g, ages 8 w) were selected and fed a week for adaptation. Then, the rats were randomly divided into blank control group (n=15) and model group (n=45). Blank control group were fed with common food, while model group were fed with high fat food, last for 8 weeks. After 8 weeks the mould was successfully made, these modules were randomly divided into: the model group, Tangzhiping group, rosiglitazone group (n=15 in each group). Tangzhiping group were given Tangzhiping formula particles according to the statement 20 g/kg·d to fill in the stomach; Rosiglitazone group were given 08 mg/kg·d rosiglitazone gavage; Model group and blank control group were given the same amount of saline solution，again for 8 weeks; 12hs after the last administration of fasting, levels of insulin sensitivity were evaluated by the “euglycemic hyperinsulin clamp technique”, and levels of body weight, fast plasma glucose, serum insulin , were observed, Meanwhile superoxide dismutase （SOD）,glutathione peroxidase （GSHPx）malonaldehyde（MDA）, and catalase（CAT）of skeletal muscle were measured． ResultsThe M level was significantly higher while mean body weight, blood glucose, and fasting insulin of Tangzhiping group were lower than that of model group,. The differences were statistically significant (P＜005), with rosiglitazone group, which body weight was statistically significant (P＜005), blood sugar and insulin had no statistical significance (P＞005); The mean SOD, GSH and CAT level of Tangzhiping group is significantly higher than model group, MDA level was lower, the differences were statistically significant (P＜005), with rosiglitazone group statistically significant (P＜005). ConclusionTangzhiping can improve insulin resistance, and also improve oxidative stress, which reflects that Tangzhiping can enhance bodys metabolic activity through improving the upstream pathway of IR—oxidative stress, in the end improve IR. Its mechanism may be through improving skeletal muscle mitochondrial ROS.

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备注/Memo

备注/Memo:
基金项目：北京市自然科学基金（7122020）
作者单位：100069北京，首都医科大学中医药学院［赵轩(硕士研究生)、高彦彬、朱智耀、耿建国、张娜（博士研究生）、周盛楠、李娇阳(硕士研究生)］
作者简介：赵轩(1986- )，女，2011级在读硕士研究生。研究方向：中医药防治糖尿病及其并发症的临床和基础研究。Email：cnxxmzj@163.com通讯作者：高彦彬(1960- )，博士，教授，主任医师，博士研究生导师。研究方向：中医药防治糖尿病及其并发症的临床和基础研究。Email：dfyynfm@163.com
更新日期/Last Update: 1900-01-01