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[1]李胜杰 李志同 杨伟宁等.电针上巨虚穴、尺泽穴对溃疡性结肠炎大鼠肺、肠肿瘤坏死因子 α 及结肠病理的比较研究[J].环球中医药,2014,7(06):415-0.
　LI Sheng jie,LI Zhi tong,YANG Wei ning,et al.Comparative study of electroacupuncture at Shangjuxu (ST 37) and Chize (LU 5) on TNFalpha content in the lung and colon and morphology of colonic mucosa in rats with ulcerative colitis[J].,2014,7(06):415-0.
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电针上巨虚穴、尺泽穴对溃疡性结肠炎大鼠肺、肠肿瘤坏死因子 α 及结肠病理的比较研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年06期
页码:
415-0
栏目:
论著
出版日期:
2014-06-06

文章信息/Info

Title:
Comparative study of electroacupuncture at Shangjuxu (ST 37) and Chize (LU 5) on TNFalpha content in the lung and colon and morphology of colonic mucosa in rats with ulcerative colitis
作者:
李胜杰 李志同 杨伟宁等
Author(s):
LI Shengjie;  LI Zhitong;  YANG Weining;  et al.
School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
电针; 上巨虚; 尺泽; 溃疡性结肠炎; 肿瘤坏死因子α
Keywords:
Electroacupuncture; Shangjuxu (ST 37); Chize (LU 5); Ulcerative Colitis; Tumor necrosis factor α
分类号:
R245.9＋7
DOI:
-
文献标志码:
A
摘要:
目的观察电针上巨虚穴、尺泽穴对溃疡性结肠炎大鼠不同器官中肿瘤坏死因子α（tumor necrosis factor α, TNFα）含量及结肠黏膜病理改变的影响，探讨大肠下合穴上巨虚及肺经合穴尺泽对溃疡性结肠炎的影响及机制。方法将Wistar雄性大鼠28只随机分为正常组、模型组、上巨虚组及尺泽组，每组7只。采用乙酸灌肠法制备溃疡性结肠炎模型。于造模后第3天起，上巨虚组电针双侧上巨虚穴，尺泽组电针双侧尺泽穴，每次15分，每天一次，频率2/100 Hz，连续治疗7天；其余两组只抓取、束缚，不做治疗，每次15分，每天一次，7天后取材。放射免疫法测定肺、结肠TNFα的含量；结肠组织HE染色，观察病理形态学变化。结果 (1) TNFα的含量：结肠组织中，模型组显著高于正常组（P＜005），上巨虚组、尺泽组显著低于模型组（P值均＜005），两治疗组比较未见显著性差异（P＞005）；肺脏中，尺泽组TNFα含量显著低于上巨虚组（P＜005），其他组间则未见显著性差异（P值均＞005）。(2) 结肠与肺的比值，上巨虚组显著低于模型组和尺泽组（P值均＜005），其他各组间未见显著性差异（P＞005）。(3) 模型组大鼠结肠光镜观察可见溃疡形成和炎症，上巨虚组和尺泽组大鼠结肠黏膜病理损伤减轻，其中上巨虚组减轻更为明显。结论(1) 上巨虚和尺泽分别作为肺经的合穴和大肠的下合穴，均可降低TNFα在结肠中的含量，有效阻抑UC所致的结肠炎症反应及改善结肠粘膜的病理改变，为“合主逆气而泄”、“合治内府”理论提供了实验依据。(2) 上巨虚与尺泽作用的特异性靶器官可能不同，上巨虚对肠源性腹泻更有效，而尺泽对于肠炎引起的肺部损伤较上巨虚更有优势。
Abstract:
ObjectiveTo probe into the mechanisms of electroacupuncture (EA) at Shangjuxu (ST 37, lower Hesea acupoint) and Chize (LU 5, Hesea acupoint) for treatment of the ulcerative colitis (UC), through observing TNFAlpha content in different organs and morphology of colonic mucosa in UC rats. MethodsTwentyeight male Wistar rats were randomly divided into 4 groups: a control group, a model group, an EAShangjuxu (EAST 37) group and an EAChize (EALU 5) group, 7 rats in each group. The UC rat model was made with by enema with 10% acetic acid, and the change of TNFα content in the Lung and Colon and morphology of colonic mucosa after EA at Shangjuxu (ST 37) and Chize (LU 5) were observed. The treatment began from day 3 after establishing models. EA(2/100HZ) was applied to bilateral Chize (LU 5) and Chize (LU 5) for 15 min, once daily for 7 days. The control group and model group bound only for 15min, once daily for 7 days. TNFα content in the lung and colon was determined by RIA. Morphology of colonic mucosa were observed under a light microscope by hematoxylineosin(HE) Staining. Results(1) EA at Shangjuxu (ST 37) and Chize (LU 5) could significantly decrease the TNFα level in the colic tissues with significant differences as compared with the model group (P＜0.05). Compared with the control group, the TNFα content was obviously increased in the model group (P＜0.05). No significant difference can be seen between EAShangjuxu group and EAChize group. The TNFα content in EAChize group was significantly decreased in the lung tissues comparing with EAShangjuxu group(P＜0.05). No significant difference can be seen among the other groups(P＞0.05).. (2) The ratio of TNF levels in the colon and lung in EAShangjuxu group was significantly decreased comparing with the model group (P＜0.05) and EAChize group(P＜0.05). No significant differences among the other groups(P＞0.05). (3) Ulceration and inflammation of colon can be observed in the model group. Pathological lesions of colonic mucosa were reduced in the EAShangjuxu group and EAChize group. The EAShangjuxu group was more obvious. Conclusions(1) EA at Shangjuxu (ST 37) and Chize (LU 5) could significantly decrease the TNFα level in the colic tissues, effectively prevent inflammatory response of colon and improving pathological changes of colonic mucosa, which has provided the experimental basis for the theory of "hesea points governing vomiting and diarrhea" and "Curing viscera diseases by hesea points". (2) Maybe Shangjuxu (ST 37) and Chize (LU 5) have different target organs. Shangjuxu (ST 37) is better for enterogenic diarrhea, while Chize (LU 5) has more advantages than Shangjuxu (ST 37) for lung injury caused by enteritis.

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备注/Memo

备注/Memo:
基金项目：北京中医药大学自主选题项目(2011JYBZZXS099)
作者单位：100029 北京中医药大学针灸推拿学院［李胜杰(硕士研究生)、李志同(硕士研究生)、杨伟宁(硕士研究生)、赵雅芳、解秸萍］
作者简介：李胜杰(1986- )，女，2011级在读硕士研究生。研究方向：腧穴特异性研究。Email: lishengjie1201@163.com
通讯作者：解秸萍(1961- )，女，博士，教授，主任医师。研究方向：腧穴特异性研究。Email: xiejieping@sina.com
更新日期/Last Update: 1900-01-01