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[1]姚海江 莫雨平 宋洪涛等.电针对慢性应激抑郁模型大鼠松果体AANAT mRNA、HIOMT mRNA表达的影响[J].环球中医药,2014,7(07):511-0.
　YAO Hai-jiang,MO Yu-ping,SONG Hong-tao,et al.The effect of electro-acupuncture on the expression of AANAT mRNA and HIOMT mRNA in pineal of chronic stress depression model rats[J].,2014,7(07):511-0.
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电针对慢性应激抑郁模型大鼠松果体AANAT mRNA、HIOMT mRNA表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年07期
页码:
511-0
栏目:
论著
出版日期:
2014-07-06

文章信息/Info

Title:
The effect of electro-acupuncture on the expression of AANAT mRNA and HIOMT mRNA in pineal of chronic stress depression model rats
作者:
姚海江 莫雨平 宋洪涛等
Author(s):
YAO Hai-jiang;  MO Yu-ping;  SONG Hong-tao;  et al.
School of acupuncture-moxibustion and tuina, Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
电针;  慢性应激;  抑郁;  褪黑素;  芳基烷化胺N-乙酰基转移酶mRNA;  羟基吲哚氧位甲基转移酶mRNA
Keywords:
Electro-acupuncture;  Chronic stress;  Depression;  Melatonin;  Arylalkylamine N-acetyltransferase mRNA;  Hydroxyindole-O-methyl-transferase mRNA
分类号:
R245
DOI:
-
文献标志码:
A
摘要:
目的 观察电针治疗对慢性应激抑郁模型大鼠行为学、血清褪黑素(melatonin, MT)以及松果体芳基烷化胺N-乙酰基转移酶(arylalkylamine N-acetyltransferase, AANAT)mRNA、羟基吲哚氧位甲基转移酶(hydroxyindole-O-methyl-transferase, HIOMT)mRNA表达的影响,探讨电针对慢性应激抑郁模型大鼠血清MT含量调节的作用机制,从而进一步充实电针抗抑郁的作用机理。方法将大鼠随机分为3组,空白组、模型组、电针组,每组10只,共30只,通过慢性温和不可预知性应激刺激结合孤养的方法复制慢性应激抑郁模型,电针组给予强度为2 Hz、1 mA的电针治疗,每次20分钟,每天1次,共电针21天。采用开野试验观察大鼠的行为学变化; 采用酶联免疫吸附法对大鼠血清中MT的含量进行检测,用Real-time PCR检测各组大鼠松果体AANAT mRNA、HIOMT mRNA的表达情况。结果 在大鼠开野试验中,与空白组比较,模型组大鼠水平运动、垂直运动的次数均显著减少(P=0.000<0.01, P=0.007<0.01),而电针可逆转此变化,与模型组相比,电针组大鼠的水平运动次数和垂直运动次数均显著增多(P=0.000<0.01, P=0.001<0.01); 模型组大鼠血清中MT含量较空白组明显降低(P=0.000<0.01),与模型组大鼠比较,电针可提高血清中MT含量(P=0.000<0.01)。与空白组相比,模型组AANAT mRNA、HIOMT mRNA的表达均显著降低(P=0.000<0.01, P=0.026<0.05),而电针组的表达较模型组均显著升高(P=0.000<0.01, P=0.000<0.01)。结论 电针可以提高慢性应激抑郁模型大鼠血清中MT含量,并且可以提高AANAT mRNA、HIOMT mRNA的表达。其中,电针提高慢性应激抑郁模型大鼠AANAT mRNA、HIOMT mRNA的表达可能是提高大鼠血清中MT含量的机制之一。
Abstract:
Objective Observe the influence of electro-acupuncture of the locomotor activity serum MT and AANAT mRNA, HIOMT mRNA of chronic unpredictable mild stress depression model rats. Discuss the biological mechanisms of the effect of electro-acupuncture on the serum MT chronically and the biological mechanisms of electro-acupuncture treating depression. Methods 30 rats are randomly divided into 3 groups: blank group, model group, EA(electro-acupuncture)group, 10 in each group. Use the chronic unpredictable mild stress combined with solitarily feeding method to make the depression model. Treat the EA group with stimulation by electro-acupuncture; the stimulus intensity of current strength is 1 mA, 2 hz and 20 minutes, once every day for a period of 21 days. Observe the locomotor activity of rats in every group by open-field experiment. Observe the content of the serum MT using ELISA. Detect AANAT mRNA and HIOMT mRNA in the pineal body of each group by real-time PCR. Result The times of cross and rear movement of the model group are significantly lower than blank group(P=0.000<0.01、P=0.007<0.01), while electro-acupuncture can reverse it, compared with the model group, the times of cross and rear movement of the EA(electro-acupuncture)group are significantly increased(P=0.000<0.01, P=0.001<0.01). Compared with the blank group, the content of serum MT significantly decreased in model group(P=0.000<0.01), compared with the model group, electro-acupuncture can increase the content of serum MT(P=0.000<0.01). Compared with the blank group, the expression of HIOMT mRNA and AANAT mRNA of model group, were significantly lower(P=0.000<0.01、P=0.026<0.05), electro-acupuncture can significantly increase the expression of HIOMT mRNA and AANAT mRNA(P=0.000<0.01, P=0.000<0.01). Conclusion Electro-acupuncture can improve the contents of serum MT in chronic unpredictable mild stress depression model rats, and can improve the expression of AANAT mRNA and HIOMT mRNA. The improvement of the expression of AANAT mRNA and HIOMT mRNA may be one of the mechanisms to improving the content of serum MT of chronic stress depression model rats.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81072860); 北京中医药大学自主课题(532/0100604212)
作者单位:100029 北京中医药大学针灸推拿学院[姚海江(博士研究生)、莫雨平(博士研究生)、宋洪涛(博士研究生)、许安萍(博士研究生)、唐银杉(博士研究生)、李志刚],基础医学院(张淑静); 北京按摩医院住院部(张婷婷)
作者简介:姚海江(1985- ),2012级在读博士研究生。研究方向:针刺干预中枢神经系统损伤的机理研究。E-mail:haijiang19860114@163.com
通讯作者:李志刚(1965- ),博士,教授,博士生导师。研究方向:针刺手法及针刺干预中枢神经系统损伤的机理研究。E-mail:lizhigang620@126.com
更新日期/Last Update: 1900-01-01