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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第7卷

期数:

2014年08期

页码:

587-0

栏目:

论著

出版日期:

2014-08-06

文章信息/Info

Title:

Saikosaponin A inducing apoptosis of nasopharyngeal carcinoma cell and its underlying mechanisms: an experimental study

作者:

王薇

Author(s):

WANG Wei.

Department of ear-nose-throat, Affiliated Hospital of Inner Mongolia Medical University, Huhhot 010059, China

关键词:

细胞外信号调节激酶; 凋亡; 柴胡皂苷A; 鼻咽癌细胞

Keywords:

Extracellular signal regulated protein kinase;  Apoptosis;  Saikosaponin A;  Nasopharyngeal carcinoma cell

分类号:

R285.5

DOI:

-

文献标志码:

A

摘要:

目的 初步研究不同浓度的柴胡皂苷A(saikosaponin A,SSA)对鼻咽癌细胞凋亡诱导作用及其机制。方法 体外常规培养人鼻咽癌5-8F细胞,将SSA溶解于含0.2%二甲基亚砜(dimethyl sulfoxide,DMSO)的Dulbecco改良Eagle培养基(dulbecco's modified eagle medium,DMEM)高糖培养基中,分别以0(对照组)、5、10和20μmol/L SSA作用于鼻咽癌细胞48小时,之后用免疫印迹法检测不同浓度的SSA对鼻咽癌细胞总的细胞外调节蛋白激酶(total extrallular signal regulated protein ...

Abstract:

Objective To investigate the effects of saikosaponin A(SSA)on p-ERK expressions and apoptosis in human nasopharyngeal carcinoma cell.Methods SH-SY5Y cells were established in vitro firstly, then SSA was dissolved in high glucose DMEM cultures which include 0.2% DMSO, and the cultures were pretreated individually with 0～10 μmol/L SSA for 48 hours, and then, the expressions of t-ERK and p-ERK were analyzed by western-blotting in the cells for different concentrations of SSA. At the same time, apoptosis levels of cells were analyzed flow cytometry.Results A significant inhibition of p-ERK was observed in 5- 8F cells after treatment with SSA from 10～20 μmol/L groups for 48 h. It also shows that SSA from 10～20 μmol/L groups could also induce the apoptosis of 5- 8F cells.Conclusion These results demonstrate that SSA can promote the apoptosis of nasopharyngeal carcinoma cells, and the mechanism may be related to the inhibition of ERK active expression.

参考文献/References:

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备注/Memo

备注/Memo:

作者单位:010059 呼和浩特,内蒙古医科大学附属医院耳鼻咽喉科(王薇)
作者简介:王薇(1971-),女,硕士,副主任医师。研究方向:鼻部肿瘤免疫调节。E-mail: huangyongabc123@163.com

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