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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第7卷

期数:

2014年09期

页码:

691-0

栏目:

论著

出版日期:

2014-09-06

文章信息/Info

Title:

Investigation on the compatibility law of in vitro transdermal absorption of transdermal system drugs based on Jinhuang Cream experiment

作者:

曹颖 王红 刘俊红等

Author(s):

CAO Ying;  WANG Hong;  LIU Jun-hong;  et al.

Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China

关键词:

金黄膏;  小檗碱;  透皮吸收;  透皮系统

Keywords:

Jinhuang Cream;  Berberine;  Percutaneous absorpton;  Transdermal system

分类号:

R944.2+1

DOI:

-

文献标志码:

A

摘要:

目的 通过进行金黄膏中小檗碱体外透皮吸收实验,探讨金黄膏中不同药对配伍^[1]对其透皮效果的影响,进一步探讨其影响机制。方法 采用体外经皮渗透法,使用透皮扩散试验仪,选择大鼠腹部皮肤为渗透屏障,以30%乙醇—磷酸盐缓冲溶液(pH=6.8)作为接受液,用高效液相色谱法测定接受液中小檗碱含量。考察全方药组及缺姜黄、白芷组(缺药方A组)和缺姜黄、白芷、天南星、陈皮、厚朴组(缺药方B组)中小檗碱的透皮特性。结果(1)缺药方A组和缺药方B组的稳态透皮速率、透皮时滞、32小时内小檗碱单位面积累积透过量都较全方高。(2)金黄散全方组的稳态透皮速率及32小时内盐酸小檗碱累积透过量都低于缺姜黄、白芷的缺药方A组,说明姜黄、白芷对小檗碱的皮肤渗透有抑制作用。(3)有天南星、陈皮、厚朴的缺药方A组的小檗碱透过量高,而透皮时滞低于缺药方B组,说明天南星、陈皮、厚朴对小檗碱的皮肤渗透有促进作用。结论 金黄膏中的姜黄、白芷和天南星、陈皮、厚朴这两组药都对金黄膏发挥疗效起到了促进作用。金黄膏中每组药对都各负其责,又相互协同,合理配伍组成一个整体的透皮系统。

Abstract:

Objective By performing berberine in vitro percutaneous absorption experiments to explore the transdermal effect of the different compatibility of drugs in the Jinhuang Cream^[1] and then to further explore the influencing mechanism.Methods In this study, in vitro percutaneous penetration method was used and transdermal diffusion test instrument was applied. Rat abdominal skin was selected as permeability barrier and 30% ethanol-phosphate buffer solution(pH=6.8)was chosen as the accepted solution..The concentration of berberine in the accepted solution was measured by HPLC method to examine the transdermal properties of the whole prescription, the lack of Turmeric, Angelica dahurica group(lack of prescription group A)and the lack of Turmeric, Angelica dahurica, Araceae, Citrus, Magnolia group(lack of prescription group B)drugs.Results(1)Compared with the whole prescription group, the indexes of steady-state permeation rate, transdermal delay, 32h accumulated berberine unit area by volume and permeability were much highert in Lack of prescription group A and lack of prescription group B.(2)The steady state permeation rate and 32h accumulated berberine unit area by volume and permeability in the whole prescription group were both lower than that in the the lack prescription group A, which demonstrated that, Turmeric, Angelica dahurica can restrain the skin penetration of berberine.(3)The steady-state permeation rate in lack of prescription group A was higher than in lack of prescription group B, while and transdermal delay in lack prescription group A was lower than that in lack prescription group B. The results suggested that Araceae, Citrus, Magnolia can promote skin penetration of berberine.Conclusion Turmeric, Angelica dahurica and Araceae, Citrus, Magnolia both sets of the drugs in Jinhuang Cream can effective promote the percutaneous absorption of golden cream. Each drug in Jinhuang Cream plays an important role on the compatibility, mutual collaboration and forming a whole transdermal system.

参考文献/References:

[1] 李建波, 张莉, 张洁. 药对配伍理论及相关研究概述[J]. 中 医杂志, 2013, 54(15): 1335-1340.
[2]国家药典委员会编. 中华人民共和国药典(一部)[M]. 北京: 中国医药科技出版社, 2010: 720.
[3]吴曦. 浅析《外科正宗》之如意金黄散[J]. 贵阳中医学院学报, 2012, 34(6): 236-237.
[4]王红. 浅析“如意金黄散(膏)”的透皮系统[J]. 天津中医药, 2005, 22(1): 57-58.
[5]王颖, 郭兰萍, 黄璐琦, 等. 姜黄、莪术、郁金的化学成分与药理作用研究进展[J]. 中国药房, 2013, 24(35): 3338-3341.
[6]吴媛媛, 蒋桂华, 马逾英, 等. 白芷的药理作用研究进展[J]. 时珍国医国药, 2009, 20(3): 625- 627.

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备注/Memo

备注/Memo:

基金项目:国家自然科学基金(81072818)
作者单位:300193 天津,天津中医药大学研究生院[曹颖(硕士研究生)]; 天津中医药大学第二附属医院外一科(王红、韩俊泉、刘斌); 天津市中西医结合急腹症研究所药物研究室(刘俊红、李棣华、吕沅姗)
作者简介:曹颖(1987- ),女,2012级在读硕士研究生。研究方向:中医外科学。E-mail: sarah1234567890@sina.com 通讯作者:王红(1965- ),博士,主任医师。研究方向:中医外科学。E-mail: ctwanghong@sina.com

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