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[1]王玥姣 贾立群 朱世杰.平肺复方对人肺腺癌A549细胞PPAR-γ信号通路的影响[J].环球中医药,2014,7(10):745-0.
　WANG Yue-jiao,JIA Li-Qun,ZHU Shi-jie..Effect on PPAR-γ signal pathway induced by Pingfei compound in human lung adenocarcinoma cell line A549[J].,2014,7(10):745-0.
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平肺复方对人肺腺癌A549细胞PPAR-γ信号通路的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年10期
页码:
745-0
栏目:
论著
出版日期:
2014-10-06

文章信息/Info

Title:
Effect on PPAR-γ signal pathway induced by Pingfei compound in human lung adenocarcinoma cell line A549
作者:
王玥姣 贾立群 朱世杰
Author(s):
WANG Yue-jiao; JIA Li-Qun; ZHU Shi-jie.
Graduate School of Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
平肺复方;  PPAR-γ信号通路;  人肺腺癌A549细胞
Keywords:
Pingfei compound;  PPAR-γ signal pathway;  Human lung adenocarcinoma A549 cells
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的 研究平肺复方对人肺腺癌A549细胞增殖及过氧化物酶体增长因子活化受体(peroxisome proliferators-activated receptor, PPAR)-γ信号通路的影响。方法 运用细胞计数试剂盒(cell counting kit- 8,CCK- 8)比色法检测平肺复方对人肺腺癌A549细胞增殖的影响,并添加PPAR-γ特异性阻断剂GW9662,观察PPAR-γ通路阻断后平肺复方对A549细胞生长的影响。结果 平肺复方生药5 mg/ml和10 mg/ml在作用24小时、48小时和72小时表现出对A549细胞增殖的抑制作用(P<0.05); 单纯加入GW9662对细胞的生长并未产生影响; 加入GW9662的平肺复方仍可对细胞生长产生抑制作用(P<0.05),但这种抑制作用明显弱于平肺复方(P<0.05)。结论 平肺复方的抗肿瘤作用机制可能部分涉及到PPAR-γ信号通路,但并不完全是通过这一条信号通路在发挥作用。
Abstract:
Objective To investigate the growth effect on human lung adenocarcinoma cell line A549 by Pingfei Compound, and the effect on PPAR-γ Signal Pathway induced by it. Methods Proliferation of A549 cells after exposure to the apozem was measured by CCK-8 assay. The PPAR-γ specificity blocker GW9662 was added. Observe if the Pingfei Compound could influence the growth of the A549 cells or not. Results The 5 mg/mL and 10 mg/mL groups presented inhibitive effect on A549 growth at 24h, 48h and 72h time points(P<0.05). GW9662 did not make any difference on cell growth,while the Pingfei Compound supplemented with GW966w showed inhibitory effect on cell growth(P<0.05). Conclusion The mechanism of anti-tumor effect of Pingfei Compound may partly involved in the PPAR-γ signal pathway. But other signal pathways may meanwhile function in the anti-tumor process.

参考文献/References:

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[2] Boitier E,Gautier JC,Roberts R.Advances in understanding the regulation of apoptosis and mitosis by peroxisome-proliferator activated receptors in pre-clinical models:relevance for human health and disease[J].comp Hepatol,2003,2(1):3.
[3] Issemam I,Green S.Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators[J].Nature,1990,347(6294):645- 650.
[4] Feige JN,Gelman L,Michalik L,et al.From molecular action to physiological outputs:peroxisome proliferator-activated receptors are nuclearreceptors at the crossroads of key cellular functions[J].Prog Lipid Res,2006,45(2): 120- 159.
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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(30973853)
作者单位:100029 北京中医药大学研究生院[王玥姣(博士研究生)]; 中日友好医院中西医结合肿瘤内科(贾立群、朱世杰)
作者简介:王玥姣(1986- ),女,2011级在读博士研究生。研究方向:肺癌的中西医结合治疗。E-mail:yuejiao_wang@126.com 通讯作者:贾立群(1962- ),博士,教授,主任医师,博士生导师。研究方向:癌症的中西医结合诊治。E-mail:liqun-jia@hotmail.com
更新日期/Last Update: 1900-01-01