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[1]覃佐爱 薛海兵 吴清明等.电针“四关”组穴及其相应单穴对卒中后抑郁模型大鼠行为学及脑单胺神经递质含量影响[J].环球中医药,2014,7(12):915-0.
　QIN Zuo-ai,XUE Hai-bing,WU Qing-ming,et al.Influence of Electroacupuncture of Bilateral “Hegu”-“Taichong” and Corresponding Single Point on Ethology and Cerebral Monoamine Neurotransmitters in Post-depression Rats[J].,2014,7(12):915-0.
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电针“四关”组穴及其相应单穴对卒中后抑郁模型大鼠行为学及脑单胺神经递质含量影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年12期
页码:
915-0
栏目:
论著
出版日期:
2014-12-06

文章信息/Info

Title:
Influence of Electroacupuncture of Bilateral “Hegu”-“Taichong” and Corresponding Single Point on Ethology and Cerebral Monoamine Neurotransmitters in Post-depression Rats
作者:
覃佐爱 薛海兵 吴清明等
Author(s):
QIN Zuo-ai;  XUE Hai-bing; WU Qing-ming; et al
Graduate Education College of the Hunan University of Chinese Medicine, Changsha, 410208, China
关键词:
四关穴;  电针;  卒中后抑郁模型大鼠;  脑单胺神经递质
Keywords:
Bilateral“Hegu”-“Taichong”;  Electroacupuncture;  Post-depression rat;  Cerebral monoamine neurotransmitters
分类号:
R245
DOI:
-
文献标志码:
A
摘要:
目的 对比观察电针“四关”组穴及其相应单穴对卒中后抑郁(post-stroke depression, PSD)模型大鼠行为学能力及脑单胺神经递质含量的影响,探讨“四关”组穴的协同效应及可能的作用机制。方法 雄性SD大鼠50只,其中空白组10只,造模成功的32只随机分为模型组、四关组、合谷组、太冲组,每组8只。采用大脑中动脉闭阻术后结合慢性不可预见性温和应激刺激及孤养法造PSD模型。治疗组大鼠分别使用电针“四关”、合谷、太冲穴治疗,30分钟/次/日,连续21天。采用糖水试验、敞箱试验观察大鼠行为学指标变化,采用高效液相色谱仪荧光检测法观察大鼠脑组织中下丘脑区去甲肾上腺素(noradrenaline, NE)、多巴胺(dopamine, DA)、5-羟色胺(5-hydroxytryptamine, 5-HT)含量。结果(1)造模后,SD大鼠行为学指标及脑单胺神经递质含量较空白组均明显减少(P<0.01);(2)治疗后,四关组及太冲组大鼠的敞箱试验数据高于模型组(P<0.01),且太冲组直立次数治疗后与空白组无差别,而合谷组基本无变化且治疗后与模型组无差异(P>0.05); 治疗三组糖水消耗比逐步上升,且合谷组治疗后达到了与空白组无差别的水平(P>0.05);(3)治疗后,四关组及太冲组大鼠脑单胺神经递质含量均显著增高(P<0.01),太冲组的NE、5-HT含量又明显高于四关组(P<0.05),而合谷组大鼠脑单胺神经递质含量显著低于四关组及太冲组(P<0.01)。结论 电针四关组穴及太冲穴均能显著增加PSD模型大鼠的行为学指标,其作用机制可能与下丘脑组织中单胺类神经递质的含量的增加有关; 但四关穴治疗该病的协同效应并不突出,其机理有待进一步研究。
Abstract:
Objective To observe the effect of electroacupuncture(EA)of “Si-guan” points[bilateral “Hegu”(LI 4)-“Taichong”(LR 3)] and corresponding single point on ethology and cerebral monoamine neurotransmitters in Post-depression(PSD)Rats; to investigate the synergistic effect and the possible mechanism of bilateral LI 4-LR 3. Methods Control group had 10 normal SD rats. 32 model SD rats were quadratic randomly divided into five groups(8 rats/group): model, EA group-1(“Si-guan” points), EA group-2(bilateral LI 4 points), EA group-3(bilateral LR 3 points). The PSD model was established by separately breeding and chronic unpredictable moderate stress(CUMS)after middle cerebral artery occlusion(MACO). EA(30 min)LI 4-LR 3, bilateral LI 4 and bilateral LR 3 were applied in group-1, EA group-2 and EA group-3 respectively, once daily, continuously for 21 days. The indexes of the behaviors of PSD model rats were measured by the methods of Open-Field test and Sucrose solution consumption test, the fluorometer detecting system of HPLC was used to mensurate the monoamine neurotransmitters of Rat Hypothalamus. Results Compared with control group, the data of ethology and monoamine neurotransmitters all decreased obviously after making model(P<0.01). Compared with the model group,the data of Open-Field test increased in EA group-1 and EA group-3(P<0.01),and there was no significant difference on rearing movement times in control group and EA group-3, but EA group-2 unchanged generally and indistinctive with model group(P>0.05). The data of Sucrose solution consumption test in EA group were all increased significantly during treatment, and there was no difference in EA group-2 and control group after treatment(P>0.05. The monoamine in EA group-1 and EA group-3 were significantly increased(P<0.01), the NE、5-HT content of EA group-3 were significantly higher than EA group-1(P<0.05), but the monoamine neurotransmitters in EA group-2 were significantly lower than EA group-1 and EA group-3(P<0.01). Conclusion Both of EA “Si-guan” points and bilateral “Taichong” points can significantly increase the Open-Field test data of PSD rats, the mechanism neurotransmitters perhaps related to the increase of monoamine in Rat Hypothalamus; But the synergistic effect in treatment of bilateral “Si-guan” points was not prominent, the mechanism is worth further studying.

参考文献/References:

[1] 张永臣, 吴富东. 四关和四关穴探析[J]. 针灸临床杂志, 2005, 21(6): 5-6.
[2] 赵倩, 王季良. 四关穴浅析[J]. 四川中医, 2013,(8): 27-28.
[3] Dafer RM, Rao M, Shareef A, et al. Post-stroke depression[J].Top Stroke Rehabit, 2008, 15(1): 13-21. [4] Fruhwald S, Loffler H, Baumhackt U, et al. Depression after cerebrovascular injury. Review and differentiation from other psychiatric complications[J]. Fortschr Neurol Psychiatr, 1999, 67(4): 155-162.
[5] Koizumi J, Yoshida Y, Nakazawa T, et al. Experimental studies of ischemic brain edema: A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area [J].Jan J Stroke, 1986, 8: 1.
[6] Longa EZ, Weistein PR, Carlson S. Reversible middle cerebral artery occlusion without craniectomy in rats [J].Stroke, 1989, 20:84.
[7] Willner P.Animal models of depression:an overview[J].Trends Pharmacol Sci, 1991, 12:131.
[8] Willner P. Validity, reliability and utility of the chronic mild stress model of depression: a 10-year review and evaluation [J]. Psychopharmacology(Berl), 1997,134(4): 319-329.
[9] 李忠仁. 实验针灸学[M]. 北京: 中国中医药出版社, 2003: 360-365.
[10] 华兴邦, 李辞蓉, 周皓良, 等. 大鼠穴位图谱[J]. 实验动物与动物实验, 1999, 1(1):1-4.
[11] Newberg AR, Davydow DS, Lee HB. Cerebrovascular disease basis of depression: post-stroke depression and vascular depression [J]. International Review of Psychiatry, 2006, 18(5): 433-441.
[12] 姜超, 许永城, 张秋娟. 卒中后抑郁症的中西医临床研究进展[J]. 中国卒中杂志, 2009, 4(8):692-698.
[13] 周逸平. 经脉-脏腑相关是经络理论的核心[J]. 针刺研究, 1999,(3): 238-241,197.
[14] 易洋, 徐放明, 谢洪武, 等. 从针刺太冲穴治疗抑郁症探讨肝经与额叶联系的静息态功能磁共振研究[J]. 中国中西医结合杂志, 2011, 31(8): 1044-1050. (收稿日期:2014- 07- 21)

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备注/Memo

备注/Memo:

基金项目:湖南省科技厅一般项目(2013SK5067) 作者单位:410208 长沙,湖南中医药大学研究生教育学院[覃佐爱(硕士研究生)、薛海兵(硕士研究生)、谭志高(硕士研究生)]; 湖南省中医院针灸中风专科(吴清明、张鹏、叶海敏)
作者简介:覃佐爱(1989- ),女,2012级在读硕士研究生。研究方向:针灸临床及治病机理研究。E-mail: 197408939@qq.com 通讯作者:吴清明(1962- ),本科,教授,硕士生导师,主任医师。研究方向:针灸治病机理及临床研究。E-mail: qmwu6789@163.com
更新日期/Last Update: 1900-01-01