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[1]李娇阳,高彦彬,周盛楠,等.通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响[J].环球中医药,2015,8(01):29-32.[doi:10.3969/j.issn.1674-1749.2015.01.008]
　LI Jiao-yang,GAO Yan-bin,ZHOU Sheng-nan,et al.Effects of Tongxinluo on VEGF expression in early diabetic nephropathy rats[J].,2015,8(01):29-32.[doi:10.3969/j.issn.1674-1749.2015.01.008]
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通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年01期
页码:
29-32
栏目:
论著
出版日期:
2015-01-06

文章信息/Info

Title:
Effects of Tongxinluo on VEGF expression in early diabetic nephropathy rats
作者:
李娇阳; 高彦彬; 周盛楠; 邹大威; 朱智耀; 张娜; 崔方强; 刘静
100069北京,首都医科大学中医药学院[李娇阳(硕士研究生)、高彦彬、周盛楠(硕士研究生)、邹大威、朱智耀、张娜(博士研究生)、崔方强(博士研究生)、刘静(硕士研究生)]; 中医络病研究北京市重点实验室(李娇阳、高彦彬、周盛楠)
Author(s):
LI Jiao-yang;  GAO Yan-bin; ZHOU Sheng-nan;  et al.
Capital Medical University School of Traditional Chinese Medicine & Beijing Key Laboratory of TCM Collateral Disease Theory Research, Beijing 100069,China Corresponding author:GAO Yan-bin,E-mail:dfyynfm@163.com
关键词:
通心络;  糖尿病肾病;  高滤过;  蛋白尿;  血管内皮生长因子
Keywords:
Tongxinluo;  Diabetic nephropathy;  Hyperfiltration;  Albuminuria;  Vascular endothelial growth factor.
分类号:
R587.2
DOI:
10.3969/j.issn.1674-1749.2015.01.008
文献标志码:
A
摘要:
目的 观察通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响。方法 采用高脂饲料+小剂量链脲佐菌素建立2型糖尿病大鼠模型,随机分为空白组10只、模型组10只、缬沙坦组10只(10mg/kg/d)和通心络组10只(0.4mg/kg/d); 治疗8周后,比较各组大鼠的空腹血糖、尿白蛋白排泄率、内生肌酐清除率及肾重指数,采用酶联免疫吸附试验测定血浆血管内皮生长因子含量,western blot及RT-PCR测定肾组织中血管内皮生长因子及其受体Flt1的表达。结果 模型组大鼠空腹血糖、尿白蛋白排泄率、内生肌酐清除率、肾重指数、血浆血管内皮生长因子含量、肾组织血管内皮生长因子及Flt1表达较空白组有不同程度的升高(P<0.05),通心络组除空腹血糖外各指标水平较模型组均显著降低(P<0.05)。结论 通心络可以有效改善早期糖尿病肾病大鼠的高滤过状态,抑制尿蛋白的排出,其作用机制与抑制血管内皮生长因子及其受体Flt1的过度表达有关。
Abstract:
Objective To observe the effect of Tongxinluo on vascular endothelial growth factor(VEGF)expression in rats with early stage of diabetic nephropathy.Methods The rat model of type 2 diabetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet for 4 weeks, 40 rats were randomly divided into 4 groups-normal group, diabetic group, valsartan treated group(10mg/kg/d)and Tongxinluo treated group(0.4mg/kg/d); After 8 weeks, the level of urinary albumin excretion rate(UAER), creatinine clearance rate(Ccr),kidney weight/body weight ratio(KW/BW)and plasma VEGF was measured and the renal expression of VEGF and Flt1 was determined by western blot and real-time PCR.Results The level of UAER, Ccr, KW/BW, VEGF an Flt1 expression in Tongxinluo treated group was lower than it in diabetic group(P<0.05),while FBG had no significant difference.Conclusion Tongxinluo can improve glomerular hyperfiltration and albuminuria excretion in rats with early stage of diabetic nephropathy; The mechanism could be related to inhibition of VEGF and Flt1 expression.

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备注/Memo

备注/Memo:
基金项目:国家重点基础研究发展计划(973计划)(2012CB518602) 作者简介:李娇阳(1990- ),女,2012级在读硕士研究生。研究方向:中医药防治糖尿病及其并发症研究。E-mail:echoccmueducn@163.com 通讯作者:高彦彬(1960- ),教授,主任医师,博士,博士生导师,研究方向:中医药防治糖尿病及其并发症的临床和基础研究。E-mail:dfyynfm@163.com
更新日期/Last Update: 2015-01-05