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[1]李忆兰,戴富林,张杰根,等.三七皂甙Rb1对急性呼吸衰竭大鼠的影响及作用机制的研究[J].环球中医药,2015,8(02):144-148.[doi:10.3969/j.issn.1674-1749.2015.02.005]
　LI Yi-lan,DAI Fu-lin,ZHANG Jie-gen,et al.Research on effect and action mechanism of Panax Notoginseng saponins Rb1 on acute respiratory failure rats[J].,2015,8(02):144-148.[doi:10.3969/j.issn.1674-1749.2015.02.005]
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三七皂甙Rb1对急性呼吸衰竭大鼠的影响及作用机制的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年02期
页码:
144-148
栏目:
论著
出版日期:
2015-02-06

文章信息/Info

Title:
Research on effect and action mechanism of Panax Notoginseng saponins Rb1 on acute respiratory failure rats
作者:
李忆兰; 戴富林; 张杰根; 武凡
072750 河北省涿州市医院肿瘤科
Author(s):
LI Yi-lan;  DAI Fu-lin;  ZHANG Jie-gen;  et al.
Oncology department, Zhuozhou city hospital, Hebei, Zhuozhou 072750, China
关键词:
三七皂苷Rb1;  急性呼吸衰竭;  膜的流动性;  细胞色素氧化酶Ⅱ;  分泌性磷脂酶A2
Keywords:
Panax Notoginseng saponins Rb1;  Respiratory failure;  Membrane fluidity;  Cytochrome oxidaseⅡ;  Secretory phospholipase A2
分类号:
R285, R563.8
DOI:
10.3969/j.issn.1674-1749.2015.02.005
文献标志码:
A
摘要:
目的 探讨三七皂甙Rb1对急性呼吸衰竭大鼠肺脏线粒体功能的影响及作用机制。方法 将Wistar大鼠随机分为4组,分别为对照组、呼吸衰竭模型组、Rb1治疗1组(低剂量组5 mg/kg)、Rb1治疗2组(高剂量组10 mg/kg),每组10只。采用舌静脉注射内毒素脂多糖(lipopolysaccharide, LPS)复制大鼠急性呼吸衰竭模型,于大鼠急性呼吸衰竭3小时后,迅速取出大鼠肺脏,低温差速离心法提取肺脏组织线粒体,用荧光偏振法检测肺脏线粒体膜的流动性和膜的微粘度。用RT-PCR测定细胞色素氧化酶ⅡmRNA表达的变化,用[3H]油酸标记的大肠杆菌作为底物,测定呼吸衰竭大鼠分泌性磷脂酶A2活性的变化。结果 大鼠呼吸衰竭肺脏线粒体膜的流动性下降,呼吸衰竭组线粒体膜细胞色素氧化酶Ⅱ基因表达增强,2小时达高峰。分泌型磷脂酶A2活性1小时增强,5小时达高峰,三七皂甙Rb1低剂量组(5 mg/kg)可使线粒体膜流动性下降,细胞色素氧化酶ⅡmRNA表达增强,三七皂甙Rb1高剂量组(10 mg/kg)与模型组相比有统计学意义(P<0.01)。结论 三七皂甙Rb1对呼吸衰竭有保护作用(P<0.05),其中高剂量组对呼吸衰竭大鼠有显著的保护作用(P<0.01)。
Abstract:
Objective To research about the function and funcition mechanism of Panax Notoginseng saponins Rb1 on the mitochondrial of lung of acute respiratory failure rats. Methods Wistar rats were randomly divided into 4 groups, including control group, respiratory failure model group, Rb1 Treatment Group 1(low dose group of 5mg/kg), Rb1 Treatment Group 2(high dose group of 10 mg/kg). There were 10 rats in each group. The lipopolysaccharide(LPS)was injected through the lingual. vein to duplicate the acute respiratory failure model of rats. 3h after the acute respiratory failure of rats, the lungs of the rats were quickly removed. After blending with the homogenizer, the low temperature differential centrifugation was used to extract the mitochondria in the lung tissue, and the membrane fluidity of mitochondria was determined. The fluorescence polarization method was used to detect the potential of mitochondrial membrane of the lung membrane fluidity.RT-PCR was used to determine the change of expression of mRNA in Cytochrome Oxidase Ⅱ. Secretory phospholipase A2(sPLA2)activity was tested by labeled E.coli membrane with[3H]oleic acid as substrate to determine the change of the activity of secretory phospholipase A2 of respiratory failure rats. Results Acute respiratory failure model of rats lung mitochondria decreased.The gene expression of cytochrome oxidaseⅡ was enhanced, and reached the peak after 2h. The membrane fluidity and the micro viscosity of membrane decreased. The activity of secretory phospholipase A2 was enhanced after 1h, and reached the peak after 5h. After the treatment with Panax Notoginseng saponins Rb1 of 5mg/kg, the expression of mRNA of cytochrome oxidase Ⅱ could be inhibited. 10 mg/kg of Rb1 has statistical significance compared with the model group. Conclusion The high dose group of Panax Notoginseng saponins Rb1 has significant protective effect on the respiratory failure rats.

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相似文献/References:

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　LI Yi-lan,ZHANG Xiao-dong,CHEN Xiao-mei.Effect of panax notoginseng saponins Rb1 on pulmonary system disease and the mechanism[J].,2016,9(02):512.[doi:10.3969/j.issn.1674-1749.2016.04.039]

备注/Memo

备注/Memo:
基金项目:河北省科学技术研究与发展计划细化项目(11276103D- 6) 作者简介:李忆兰(1977- ),女,本科,主治医师。研究方向:三七皂甙Rb1对急性呼吸衰竭的作用机理。E-mail: 1634772023@qq.com 通讯作者:戴富林(1947- ),硕士,主任医师。研究方向:急慢性呼吸衰竭的机理研究。E-mail: 1634772023@qq.com
更新日期/Last Update: 2015-02-20