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[1]韩虹娟,李冬华,钱睿亚,等.从全基因表达谱角度探讨理冲汤抑制人子宫肌瘤细胞的作用机制[J].环球中医药,2015,8(03):290-295.[doi:10.3969/j.issn.1674-1749.2015.03.007]
　HAN Hong-juan,LI Dong-hua,QIAN Rui-ya,et al.Study of molecular mechanism of Lichong Decoction inhibiting human uterine leiomyoma cells based on analysis of gene expression profiles[J].,2015,8(03):290-295.[doi:10.3969/j.issn.1674-1749.2015.03.007]
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从全基因表达谱角度探讨理冲汤抑制人子宫肌瘤细胞的作用机制()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年03期
页码:
290-295
栏目:
论著
出版日期:
2015-03-06

文章信息/Info

Title:
Study of molecular mechanism of Lichong Decoction inhibiting human uterine leiomyoma cells based on analysis of gene expression profiles
作者:
韩虹娟; 李冬华; 钱睿亚; 许昕; 黄玉华; 倪婧; 张武芳; 耿建国; 赵文景
100069 首都医科大学中医药学院[韩虹娟(硕士研究生)、李冬华、张武芳、耿建国]; 首都医科大学附属北京妇产医院妇幼保健院(钱睿亚); 首都医科大学附属北京中医医院妇科(许昕、黄玉华、赵文景); 首都医科大学附属复兴医院月坛社区卫生服务中心中医科(倪婧)
Author(s):
HAN Hong-juan; LI Dong-hua; QIAN Rui-ya; et al.
Department of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China Corresponding author:LI Dong-hua,E-mail:dududoctor@163.com
关键词:
理冲汤;  子宫肌瘤;  基因表达谱;  分子机制
Keywords:
Lichong Decoction;  Uterine leiomyoma;  Gene expression profile;  Molecular mechanism
分类号:
R285
DOI:
10.3969/j.issn.1674-1749.2015.03.007
文献标志码:
A
摘要:
目的 从全基因表达谱角度探讨理冲汤抑制人子宫肌瘤细胞的作用机制。方法 采用胶原酶消化法培养子宫肌瘤细胞,并给予理冲汤大鼠含药血清进行干预,提取肌瘤细胞总RNA,运用Affymetrix GeneChip PrimeViewTM全基因芯片检测基因表达谱的变化,并进行差异表达基因、基因本体(gene ontology,GO)、信号通路等方面的分析,最后采用实时定量PCR(Realtime PCR,RT-PCR)对差异基因的表达进行验证。结果 结果显示,理冲汤组与空白对照组比较后差异表达基因数量为1154个(P<0.05,上调基因Fold change>1.5,下调基因Fold change<0.5),其中上调基因630个,下调基因524个; 富集的GO功能共有119条,主要涉及到细胞外基质的改变、细胞凋亡的诱导、细胞黏附等多方面的功能; 差异基因富集的信号通路有73条,同时,RT-PCR验证结果也与基因芯片结果趋势一致。结论 理冲汤对人子宫肌瘤的治疗作用机制是通过对多基因、多功能、多信号通路的调控而实现的。
Abstract:
Objective To investigate the molecular mechanism of Lichong Decoction(LCD)inhibiting human uterine leiomyoma cells through the analysis of differential gene expression profiles of uterine leiomyoma cells. Methods Using collagenase digestion cultured uterine leiomyoma cells,and treated by rat serum containing of LCD for 48 hours. Then the pathological changes of uterine leiomyoma cells were observed, and total RNA of uterine leiomyoma cells were extracted. The gene expression profile was detected by whole genome chip testing, then differentially expressed genes(DEGs), gene ontology(GO)and signal pathway were analyzed. The mRNA expressions were verified by real-time PCR(RT-PCR). Results Compared with the control group, the growth of LCD group cells significantly inhibited by microscope. The DEGs between the LCD group and the control group(P<0.05, Up Regulated Genes Fold change>1.5, Down Regulated Genes Fold change<0.5)were 1154, included 630 up-regulated and 524 down-regulated genes. The GO enrichment analysis(P<0.05)showed 119 functions, included extracellular matrix, induction of apoptosis, negative regulation of angiogenesis, cell adhesion, and so on. The signal pathway analysis showed 73 enriched pathways(P<0.05), The verification of mRNA expression by RT-PCR was consistent with that of microarray. Conclusion LCD treats uterine leiomyoma through the regulation of multiple genes and multiple signal pathways.

参考文献/References:

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相似文献/References:

[1]邢峰丽,封若雨,孙芳,等.消癥散积方对子宫肌瘤大小及性激素水平的影响[J].环球中医药,2017,10(12):1528.[doi:10.3969/j.issn.1674-1749.2017.12.034]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81373812,81073096) 作者简介:韩虹娟(1987- ),女,2012级硕士研究生。研究方向:中药防治妇科肿瘤机制研究。E-mail:hanhongjuan0508@126.com 通讯作者:李冬华(1972- ),女,博士,教授,硕士生导师。研究方向:中药防治妇科肿瘤机制研究。E-mail:dududoctor@163.com
更新日期/Last Update: 2015-03-20