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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第8卷

期数:

2015年03期

页码:

299-303

栏目:

论著

出版日期:

2015-03-06

文章信息/Info

Title:

Volume-when-effect influence of hovenia dulcis thunb and flower of kudzuvine compatibility to ethanol concentration and liver alcohol dehydrogenase activity of experimental alcoholic liver disease of rats

作者:

刘明; 陈绍红; 钟赣生; 柳海艳; 赵桐

100029 北京中医药大学基础医学院方药系[刘明(硕士研究生)、陈绍红、钟赣生、柳海艳、赵桐(硕士研究生)]

Author(s):

LIU Ming; CHEN Shao-hong; ZHONG Gan-sheng;  et al.

School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029,China Corresponding author: ZHONG Gan-sheng,E-mail:zhonggansheng@souhu.com

关键词:

葛花;  枳椇子;  酒精性肝损伤;  乙醇浓度;  乙醇脱氢酶活性

Keywords:

Flower of kudzuvine;  Hovenia dulcis thunb;  Alcoholic liver disease;  Ethanol concentration;  Alcohol dehydrogenase activity

分类号:

R285.5

DOI:

10.3969/j.issn.1674-1749.2015.03.009

文献标志码:

A

摘要:

目的 观察不同剂量葛花、枳椇子2:1比例配伍后解酒保肝的量-时-效关系,明确葛花枳椇子配伍使用的最佳条件。方法 采用56°红星二锅头白酒灌胃复制慢性酒精性肝损伤动物模型的方法,将Wistar雄性大鼠,按体重随机分为六组:空白组,模型组,东宝肝泰对照组,葛花枳椇子2:1高剂量配伍组,葛花枳椇子2:1中剂量配伍组,葛花枳椇子2:1低剂量配伍组,于实验4周、8周、12周末分别取材,腹主动脉取血,并迅速取肝左叶组织约0.2 g,检测血中乙醇浓度以及肝中乙醇脱氢酶活性。结果 给药8周和12周,葛花枳椇子各剂量配伍组均可降低血中乙醇浓度,增强肝中乙醇脱氢酶活性,且配伍低剂量组较其他组有一定的治疗优势。结论 结果表明,不同剂量葛花、枳椇子比例配伍均有一定程度解酒保肝功效,但葛花、枳椇子2:1比例低剂量配伍效果最佳,随着给药时间的延长治疗效果越好。

Abstract:

Objective To identify the best condition of liver protection by observing the volume - effect relationship of different doses of 2:1 ratio compatibility of hovenia dulcis thunb and flower of kudzuvine after liquor solution. Methods 56° hongxing erguotou liquor was used to lavage the six groups to establish the chronic alcoholic liver injury animal models.According to the weight, the male Wistar rats were randomly divided into six groups: blank group, model group, dongbao gantai controlled group, high dose group, moderate dose group and low dose group of 2:1 compatibility flower of kudzuvine and hovenia dulcis thunb. The blood was taken from abdominal aorta after 4 weeks, 8 weeks and 12 weeks respectively, then 0.2g of the left hepatic lobe was also taken to detect the concentration of ethanol in blood and liver alcohol dehydrogenase activity. Results Administration of 8 weeks and 12 weeks, different doses of flower of kudzuvine and hovenia dulcis thunb can reduce the blood compatibility of ethanol concentration, enhance the activities of ADH in liver. Findings show that low dose group outweighs other groups in terms of efficacy.Conclusion Findings also indicate that different doses of hovenia dulcis thunb and flower of kudzuvine compatibility can protect liver, but the best model is the low dose group, and the longer medicine administration, the better efficacy it will be.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目:国家自然科学基金(81274104) 作者简介:刘明(1987- ),女,2012级在读硕士研究生。研究方向:中药治疗酒精性疾病的研究。E-mail:731627836@qq.com 通讯作者:钟赣生(1961- ),硕士,教授,博士生导师。研究方向:中药治疗酒精性疾病的研究。E-mail:zhonggansheng@souhu.com

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更新日期/Last Update: 2015-03-20