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[1]李家立,李平,马洪皓,等.连夏配方颗粒对心肌梗死后大鼠心梗周围区神经生长因子mRNA、酪氨酸激酶受体A mRNA表达的影响[J].环球中医药,2015,8(03):303-306.[doi:10.3969/j.issn.1674-1749.2015.03.010]
　LI Jia-li,LI Ping,MA Hong-hao,et al.Effects of LianXia Formula Granule on NGF mRNA and TrKA mRNA expression level at infarcted border zone of rats after myocardial infarction[J].,2015,8(03):303-306.[doi:10.3969/j.issn.1674-1749.2015.03.010]
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连夏配方颗粒对心肌梗死后大鼠心梗周围区神经生长因子mRNA、酪氨酸激酶受体A mRNA表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年03期
页码:
303-306
栏目:
论著
出版日期:
2015-03-06

文章信息/Info

Title:
Effects of LianXia Formula Granule on NGF mRNA and TrKA mRNA expression level at infarcted border zone of rats after myocardial infarction
作者:
李家立; 李平; 马洪皓; 白芳芳; 田辰; 郝腾; 赵利; 朱国东
100029 北京中医药大学[李家立(博士研究生)、田辰、郝腾、赵利、朱国东(硕士研究生)]; 北京中医药大学第三附属医院心内科(李平、白芳芳),重症监护室(马洪皓)
Author(s):
LI Jia-li;  LI Ping;  MA Hong-hao;  et al.
Beijing University of Chinese Medicine, Beijing 100029, China Corresponding author: LI Ping, E-mail: pearll2008@126.com
关键词:
连夏配方颗粒;  心肌梗死;  自主神经重构;  神经生长因子;  酪氨酸激酶受体A
Keywords:
LianXia Formula Granule;  Myocardial Infarction;  Cardiac Autonomic Neural Remodeling;  Nerve growth factor;  Tyrosine kinase receptor A
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.03.010
文献标志码:
A
摘要:
目的 观察连夏配方颗粒对心肌梗死后大鼠心梗周围区心肌组织神经生长因子(nerve growth factor, NGF)mRNA、酪氨酸激酶受体A(tyrosine kinase receptor A,TrKA)mRNA表达的影响。方法 将90只清洁级健康7周龄Wistar雄性大鼠随机分为假手术组、心梗模型组、美托洛尔组、连夏配方颗粒高、中、低剂量(189.00、94.50、47.25 mg/kg)组。各组均结扎左冠状动脉前降支以复制心肌梗死大鼠模型,假手术组只穿线,不结扎。假手术与心梗模型组大鼠予生理盐水,余各组予相应实验药物; 连续灌胃给药30天,采用Real-time RT-PCR方法检测心梗周围区NGF mRNA、TrKA mRNA表达水平。结果 与假手术组比较,心梗模型组大鼠心梗周围区心肌组织NGF mRNA、TrKA mRNA表达均明显增高(P<0.01); 与模型组比较,连夏配方颗粒高、中、低剂量组大鼠心梗周围区NGF mRNA表达均明显降低(P<0.01); 连夏配方颗粒高、中剂量组大鼠心梗周围区TrKA mRNA表达均明显降低(P<0.01); 与美托洛尔组比较,连夏配方颗粒高剂量组大鼠心梗周围区NGF mRNA表达明显降低(P<0.05)。结论 连夏配方颗粒可显著降低心肌梗死后大鼠心梗周围区心肌组织NGF mRNA、TrKA mRNA的表达,达到改善心肌梗死后大鼠心梗周围区自主神经重构的作用。
Abstract:
Objective To investigate the effects of LianXia Formula Granule(LXFG)on NGF mRNA、TrKA mRNA expression level at infarcted border zone of rats after myocardial infarction(MI). Methods Ninty healthy male Wistar rats were randomly assigned to six groups: sham operated group, model group, metoprolol group, high, middle and low-dose of LXFG group(189.00, 94.50, 47.25 mg/kg). The sham operated rats were not ligated in the left anterior descending coronary artery except the rest groups. The sham operated group and model group were given with normal saline by intragastric administration, and the rest groups were given with medicine for testing. 30 days later, NGF mRNA, TrKA mRNA expression level were separately measured in all groups. Results Compared with the model group, NGF mRNA expression level in the high, middle and low-dose of LXFG group decreased(P<0.01)and TrKA mRNA expression level in the high and middle-dose of LXFG group decreased(P<0.01). Compared with the metoprolol group, NGF mRNA expression level in the high-dose of LXFG group decreased(P<0.05). Conclusion Application of LianXia Formula Granule in rats after MI can significantly decrease the expression level of NGF mRNA、TrKA mRNA to improve cardiac autonomic neural remodeling.

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备注/Memo

备注/Memo:
基金项目:国家“十一五”科技支撑计划(2007BAI10B027); 北京中医药大学自主选题(2013-ZYLC- 015); 北京中医药大学自主选题(2013-JYBZZ-XS-170) 作者简介:李家立(1985- ),2012级在读博士研究生。研究方向:中医药防治心血管系统疾病。E-mail: lijiali5678@163.com 通讯作者:李平(1965- ),女,博士,主任医师,博士生导师。研究方向:中医药防治心血管系统疾病。E-mail: pearll2008@126.com
更新日期/Last Update: 2015-03-20