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[1]孔祥茹,李棣华,杜潇,等.半夏泻心汤对MNNG诱导大鼠前胃鳞癌的防治作用[J].环球中医药,2015,8(04):385-389.[doi:10.3969/j.issn.1674-1749.2015.04.001]
　KONG Xiang-ru,LI Di-hua,DU Xiao,et al.Preventive and therapeutic effects of Banxia Xiexin Decoction on rats with forestomach squamous carcinoma induced by MNNG[J].,2015,8(04):385-389.[doi:10.3969/j.issn.1674-1749.2015.04.001]
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半夏泻心汤对MNNG诱导大鼠前胃鳞癌的防治作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年04期
页码:
385-389
栏目:
论著
出版日期:
2015-04-06

文章信息/Info

Title:
Preventive and therapeutic effects of Banxia Xiexin Decoction on rats with forestomach squamous carcinoma induced by MNNG
作者:
孔祥茹; 李棣华; 杜潇; 高望; 宋清武; 李慧臻
作者单位:300193 天津中医药大学研究生院[孔祥茹(硕士研究生)、杜潇)]; 天津中医药大学第二附属医院脾胃病科(李慧臻、高望、宋清武); 天津市中西医结合医院急腹症研究所(李棣华)
Author(s):
KONG Xiang-ru; LI Di-hua; DU Xiao; et al.
Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China Corresponding author:LI Hui-zhen,E-mail:ctjenny@126.com
关键词:
半夏泻心汤;  前胃;  鳞癌;  N-甲基-N'-硝基-N-亚硝基胍;  防治
Keywords:
Banxia Xiexin Decoction;  Forestomach;  Squamous cell carcinomas;  N-methyl-N'-nitro-N-nitrosoguanidine;  Preventive and therapeutic effect
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.04.001
文献标志码:
A
摘要:
目的 观察半夏泻心汤对N-甲基-N'-硝基-N-亚硝基胍(N-methyl-N'-nitro-N-nitrosoguanidine,MNNG诱导大鼠前胃鳞癌的防治作用。方法 将140只雄性SD大鼠随机分为3组,空白组20只、模型组100只、模型中药组20只,采用MNNG联合多因素造模。每4周抽检模型组大鼠4只,观察前胃黏膜组织学变化,确定造模是否成功。造模成功后抽检各组大鼠,并将剩余模型组39只大鼠随机分为4组,模型对照组9只,半夏泻心汤高、中、低剂量组各10只,分别给予0.9%生理盐水、2.29、1.375、0.6875 g/mL的半夏泻心汤灌胃,10 mL/kg,1次/天,连续12周。40周末,处死全部大鼠,观察前胃黏膜组织学变化。结果 28周末前胃鳞状上皮增生、异型增生、鳞癌发生率,空白组为5%、5%、0,模型组为100%、83.3%、50%,模型中药组为0、0、0。40周末前胃鳞状上皮增生、异型增生、鳞癌发生率,模型对照组为100%、100%、77.8%,高剂量组为100%、85.7%、57.1%,中剂量组为100%、44.5%、22.3%,低剂量组为100%、77.8%、66.7%。结论 半夏泻心汤对MNNG多因素造模法诱发大鼠前胃鳞癌的发生具有一定的防治作用。
Abstract:
Objective To observe the preventive and therapeutic effects of Banxia Xiexin Decoction on rats with forestomach squamous carcinoma induced by MNNG. Methods 140 SD rats were randomly divided into 3 groups: control group(20 rats), model group(100 rats), and model and traditional Chinese medicine(TCM)group(20 rats). The rat models were established with rats in all the groups except control group by using combination MNNG and multiple factors methods. 4 rats were randomly tested by observing on the histological changes of forestomach mucosa every 4 weeks, in order to determine whether the rat models establishing was successful. After rat models establishment, there were 39 rats left in model group, and the rats were randomly divided into 4 groups: model control group(9 rats, received gavage administration with normal saline), high, middle and low dosage of Banxia Xiexin Decoction group(with 10 rats in each group, received gavage administration with Banxia Xiexin Decoction with dosage of 2.29 g/mL, 1.375 g/mL, and 0.6875 g/mL). The volume of gavage administration was 10 mL/(kg·d), and the treatment lasted for 12 weeks. At the end of the 40th week, all the rats were sacrificed, in order to observe the histological changes of forestomach mucosa. Results At the end of the 28th week, the incidence rate of squamous epithelium hyperplasia, atypical hyperplasia and squamous carcinoma in forestomach was 5%, 5% and 0 respectively of rats in control group, model group was 100%, 83.3% and 50% respectively, while model and TCM group was 0, 0 and 0 respectively. At the end of the 40th week, the incidence rate of squamous epithelium hyperplasia, atypical hyperplasia and squamous carcinoma in forestomach of rats in model control group was 100%, 100% and 77.8% respectively, and these of rats in high dosage of Banxia Xiexin group was 100%, 85.7% and 57.1%, middle dosage of Banxia Xiexin group was 100%, 44.5% and 22.3%, and low dosage of Banxia Xiexin group was 100%, 77.8% and 66.7%. Conclusion There was a certain preventive and therapeutic effects of Banxia Xiexin decoction on rats with forestomach squamous carcinoma induced by MNNG.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(8123708); 天津市高校“中青年骨干创新人才培养计划” 作者简介:孔祥茹(1989- ),2012级在读硕士研究生。研究方向:中医内科学的临床与实验研究。E-mail:442709060@qq.com 通讯作者:李慧臻(1965- ),女,博士,主任医师,副教授,硕士生导师。研究方向:慢性萎缩性胃炎癌前病变的中医药治疗及实验研究。E-mail:ctjenny@126.com
更新日期/Last Update: 2015-04-20