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[1]徐睿鑫,李绍旦,刘毅,等.补肾活血颗粒对帕金森病模型大鼠黑质纹状体bcl-2、bax表达的影响[J].环球中医药,2015,8(04):415-418.[doi:10.3969/j.issn.1674-1749.2015.04.008]
　XU Rui-xin,LI Shao-dan,LIU Yi,et al.The effects of Bushen huoxue Granule on the expressions of bcl-2 and bax in substantia nigra and striatum in rats models with Parkinson's disease[J].,2015,8(04):415-418.[doi:10.3969/j.issn.1674-1749.2015.04.008]
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补肾活血颗粒对帕金森病模型大鼠黑质纹状体bcl-2、bax表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年04期
页码:
415-418
栏目:
论著
出版日期:
2015-04-06

文章信息/Info

Title:
The effects of Bushen huoxue Granule on the expressions of bcl-2 and bax in substantia nigra and striatum in rats models with Parkinson's disease
作者:
徐睿鑫; 李绍旦; 刘毅; 于磊; 杨明会
作者单位:100853 北京,解放军医学院[徐睿鑫(硕士研究生)、于磊(硕士研究生)]; 中国人民解放军总医院中医院(李绍旦、刘毅、杨明会)
Author(s):
XU Rui-xin;  LI Shao-dan; LIU Yi; et al.
Chinese PLA Medical School,Beijing 100853,China Corresponding author: YANG Ming-hui,E-mail: ymh9651@ sina.com
关键词:
帕金森病;  补肾活血颗粒;  B淋巴细胞瘤-2;  Bax蛋白;  大鼠黑质纹状体
Keywords:
Parkinson's disease;  Bushen huoxue Granule;  B-cell lymphoma-2;  bax protein;  Substantia nigra and striatum in rats
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.04.008
文献标志码:
A
摘要:
目的 探讨补肾活血颗粒对帕金森病模型大鼠黑质纹状体中B淋巴细胞瘤-2蛋白(B-cell lymphoma-2, bcl-2)、bax表达的影响。方法 33只雄性SD大鼠随机分为生理盐水正常组、6-羟基多巴胺模型组和补肾活血颗粒治疗组,应用免疫组织化学染色技术观察大鼠黑质纹状体中bcl-2、bax表达的变化情况。结果 模型组bcl-2在黑质和纹状体的细胞阳性表达率增殖指数(proliferation index,PI)分别为0.43±0.022、1.14±0.057,均低于正常组(均P<0.01)和治疗组(均P<0.01)。模型组bax在黑质和纹状体的PI值分别为2.07±0.104、2.86±0.143,均高于正常组(均P<0.01)和治疗组(均P<0.01)。结论 补肾活血颗粒治疗帕金森病的疗效机制可能与上调帕金森病大鼠黑质纹状体系统bcl-2蛋白表达及下调bax蛋白表达有关。
Abstract:
Objective To observe the effects of Bushen Huoxue Granule(BSHXG)on the expressions of B-cell lymphoma-2(bcl-2)and bax in substantia nigra and striatum in rats model with Parkinson's disease(PD). Methods Thirty-three male SD rats were randomly divided into 6-OHDA-induced PD model group, BSHXG treatment group and normal control group. Changes in the expressions of bcl-2 and bax in substantia nigra and striatum in rats were observed by means of immunohistochemical staining. Results bcl-2's proliferation indexes of positive expression rates of the cells in the substantia nigra of the PD model group and the striatum of the PD model group were respectively 0.43 ± 0.022,1.14 ± 0.057, both lower than the normal group(both P<0.01)and treatment group(both P<0.01)while bax's PI in the substantia nigra of the PD model group and the striatum of the PD model group were respectively 2.07±0.104、2.86±0.143, both higher than the normal group(both P<0.01)and treatment group(both P<0.01). Conclusion The therapeutic effect of BSHXG on PD may be associated with up regulating the expression of bcl-2 protein and down regulating the expression of bax protein in substantia nigra and striatum in rats with Parkinson's disease.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81273969)、北京市科技计划“十病十药”研发专项(Z131100002513015) 作者简介:徐睿鑫(1986- ),2012级在读硕士研究生。研究方向:中西医结合临床研究。E-mail:xrxxrx@163.com 通讯作者:杨明会(1962- ),博士,教授,主任医师,博士生导师。解放军中医药学会会长。研究方向:中医及中西医结合临床及基础研究。E-mail: ymh9651@ sina.com
更新日期/Last Update: 2015-04-20