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[1]柳洋 朱跃兰 侯秀娟 刘小平.芍甘附子汤加味对胶原诱导性关节炎大鼠滑膜血管新生相关因子表达的影响[J].环球中医药,2015,8(06):692-696.[doi: A doi:10.3969/j.issn.1674-1749.2015.06.013]
　LIU Yang,ZHU Yue-lan,HOU Xiu-juan,et al.Effects of modified Shaogan Fuzi Decoction on angiogenesis relating factors in the synovial tissue of CIA rats[J].,2015,8(06):692-696.[doi: A doi:10.3969/j.issn.1674-1749.2015.06.013]
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芍甘附子汤加味对胶原诱导性关节炎大鼠滑膜血管新生相关因子表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年06期
页码:
692-696
栏目:
论著
出版日期:
2015-06-06

文章信息/Info

Title:
Effects of modified Shaogan Fuzi Decoction on angiogenesis relating factors in the synovial tissue of CIA rats
作者:
柳洋 朱跃兰 侯秀娟 刘小平
100029 北京中医药大学东方医院风湿免疫科[柳洋(硕士研究生)、朱跃兰、侯秀娟、刘小平]
Author(s):
LIU Yang;  ZHU Yue-lan;  HOU Xiu-juan; et al.
Dongfang HosPital of Beijing University of Chinese Medicine, Beijing 100078, China
关键词:
缺氧诱导因子1α;  血管生成素1;  血管生成素2;  胶原诱导性关节炎;  血管新生
Keywords:
HyPoxia-inducible factor-1α;  AngioPoietin-1;  AngioPoietin-2;  Collagen-induced arthritis;  Angiogenesis
分类号:
R285.5
DOI:
A doi:10.3969/j.issn.1674-1749.2015.06.013
文献标志码:
A
摘要:
目的 观察芍甘附子汤加味对胶原诱导性关节炎大鼠滑膜组织缺氧诱导因子1α(hypoxia-inducible factor-1α,HIF-1α)、血管生成素1(angiopoietin-1,Ang-1)和血管生成素2(angiopoietin-2,Ang-2)的影响,探讨其治疗类风湿关节炎的抗血管新生作用机制。方法 选用健康Wistar大鼠30只,随机分为正常组、模型组、对照组(雷公藤多甙片组)和小、中、大剂量治疗组(芍甘附子汤加味组),除正常组均制备胶原诱导性关节炎(Collagen-induced arthritis, CIA)模型,造模成功后连续给药28天。测定各组大鼠足趾肿胀体积,进行关节炎指数(Arthritis index, AI)评分,采用免疫组织化学方法检测大鼠膝关节滑膜组织HIF-1α、Ang-1和Ang-2的表达。结果 与模型组比较,对照组和大剂量治疗组足趾肿胀体积有所降低(P<0.05),对照组和中、大剂量治疗组AI评分明显降低(P<0.01); 与正常组比较,模型组HIF-1α和Ang-1表达均显著升高(P<0.01),Ang-2表达有所升高(P<0.05); 与模型组比较,对照组和大、中剂量治疗组HIF-1α和Ang-1表达均出现显著降低(P<0.01),Ang-2表达呈降低趋势。结论 芍甘附子汤加味可能通过降低类风湿关节炎滑膜组织HIF-1α、Ang-1和Ang-2的表达,抑制滑膜血管新生,从而起到治疗类风湿关节炎的作用。
Abstract:
Objective The Purpose of the present study was to examine the effects of modified Shaogan Fuzi Decoction on the expressions of hypoxia-inducible factor-1α(HIF-1α), angiopoietin-1(Ang-1)and angiopoietin-2(Ang-2)in the synovial tissue of rats with collagen-induced arthritis(CIA), and to investigate the anti-angiogenesis mechanism of modified Shaogan Fuzi Decoction in treating rheumatoid arthritis(RA). Methods Thirty healthy female Wistar rats were randomly divided into 6 groups: contral group, model group, tripterygium glucoside group and low, middle and high dose treatment group of modified Shaogan Fuzi Decoction. After the CIA rat model was successfully established, rats were intragastrically administrated with distilled water in the control group or medicine in the treatment groups for twenty-eight days. The toe swell volume was measured; the arthritis index(AI)was evaluated and the expression levels of HIF-1α, Ang-1 and Ang-2 were observed by immunohistochemical staining. Results Compared to that of in the model group, the toe swell volume of the tripterygium glucoside group and the high dose group significantly reduced(P<0.05); the AI score of the tripterygium glucoside group and the middle, high dose groups significantly reduced compared to the model group(P<0.01); Compared to the control group, the expression levels of HIF-1α, Ang-1 and Ang-2 significantly reduced in the model group(P<0.01, P<0.05); the expression levels of HIF-1α and Ang-1 significantly decreased in the tripterygium glucoside group and the high and middle groups compared to the model group(P<0. 01), and Ang-2 expression showed a downward trend. Conclusion The experimental data implied that modified Shaogan Fuzi Decoction plays an important role in treating RA via the way of inhibiting angiogenesis in synovial tissue by down-regulating the expressions of HIF-1α, Ang-1 and Ang-2.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:柳洋(1988- ),女,2012级在读硕士研究生。研究方向:中医药防治风湿免疫病。E-mail: jasmine701609@163.com 通讯作者:朱跃兰(1959- ),女,博士,主任医师,博士生导师。研究方向:中医药防治风湿免疫病。E-mail: zhuyuelanting@163.com
更新日期/Last Update: 1900-01-01