|本期目录/Table of Contents|

[1]刘鹏年 李忆兰 张路 谢飞 李剑瑜 武凡.三七皂甙Rb1 对大鼠慢性阻塞性肺疾病线粒体的防护作用[J].环球中医药,2015,8(07):794-797.[doi:doi:10.3969/j.issn.1674-1749.2015.07.009]
　LIU Peng-nian,LI Yi-lan,ZHANG Lu,et al.Protective effect of panax notoginseng saponins Rb1 on mitochondria of rats with chronic obstructive pulmonary disease[J].,2015,8(07):794-797.[doi:doi:10.3969/j.issn.1674-1749.2015.07.009]
点击复制

三七皂甙Rb1 对大鼠慢性阻塞性肺疾病线粒体的防护作用()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年07期
页码:
794-797
栏目:
论著
出版日期:
2015-07-06

文章信息/Info

Title:
Protective effect of panax notoginseng saponins Rb1 on mitochondria of rats with chronic obstructive pulmonary disease
作者:
刘鹏年 李忆兰 张路 谢飞 李剑瑜 武凡
072750 河北省涿洲市医院检验科(刘鹏年、张路、谢飞、武凡),输血科(李剑瑜),肿瘤科(李忆兰)
Author(s):
LIU Peng-nian;  LI Yi-lan;  ZHANG Lu;  et al.
Clinical laboratory, Zhuozhou hospital, Zhuozhou 072570, china
关键词:
三七皂甙Rb1;  慢性阻塞性肺疾病;  线粒体
Keywords:
Panax notoginseng saponins Rb1;  Chronic obstructive pulmonary disease;  Mitochondrial
分类号:
R285.5
DOI:
doi:10.3969/j.issn.1674-1749.2015.07.009
文献标志码:
A
摘要:
目的 探讨三七皂甙Rb1对大鼠慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)线粒体的保护作用及作用机理。方法 将40只大鼠随机分为4组,分别为对照组、COPD组、Rb1 10 mg/kg组和Rb1 20 mg/kg组。用烟熏法复制COPD大鼠模型,用低温差速离心法提取肺脏组织线粒体,紫外分光光度法测定肺脏线粒体呼吸链复合体Ⅰ、Ⅱ、Ⅲ、Ⅳ的活性及ATP合酶活性。用荧光分光光度计测量肺脏线粒体的膜电位,采用荧光标记与荧光偏振法测定线粒体膜偏振度,计算膜
Abstract:
Objective To investigate the protection mechanism of panax notoginseng saponins Rb1 on mitochondria of rats with chronic obstructive pulmonary disease(COPD).Methods 40 rats were randomly divided into 4 groups: control group, COPD group, 10 mg/kg Rb1 group and 20 mg/kg Rb1 group.COPD rat models were established by using smoking method.Mitochondrias in rat lungs were extracted by using hypothermal differential centrifugation method, and the activity of mitochondrial respiratory complex Ⅰ,Ⅱ,Ⅲ and Ⅳ in lungs was determined by using ultraviolet spectroscopy.Pulmonary mitochondrial membrane potential(MMP)was determined by using fluorescence spectrophotometer.Polarization degree of mitochondrial membrane and microviscosity of membrane were determined and calculate by using fluorescence tagging and fluorescence polarization methods.The activity of secretory phospholipase A2(sPLA2)secreted by pulmonary mitochondria was tested by using ELISA.Results The mitochondrial membrane potential and membrane fluidity were decreased, and polarization degree and microviscosity of mitochondrial membrane were increased in rats in COPD group.The activity of mitochondrial respiratory complex Ⅰ,Ⅲ and Ⅳ and ATP synthase were increased significantly in rats in Rb1 group(P<0.01).An increase in mitochondrial membrane potential and membrane fluidity and a decrease in polarization degree and microviscosity of mitochondrial membrane in rats of Rb1 group was proved by biophysiology.10 mg/kg and 20 mg/kg Rb1could inhibit the sPLA2 activity(P<0.05, P<0.01).Conclusion Panax notoginseng saponins Rb1 could prevent and treat COPD through protective effect on pulmonary mitichondrial structure and function of rats.

参考文献/References:

[1] 陆燕,潘旭.三七总皂甙中代表性皂苷含量测定方法学的建立及稳定性研究[J].中国药理学通报,2014,4(11):88-91. [br/] [2] 包金凤,张晓文,卢新政,等.参三七皂甙Rb1、Rg1预适应对肥厚心肌细胞缺氧复氧损伤细胞凋亡的影响[J].甘肃医药,2010,29(2):121-124. [br/] [3] Angelis N,Porpodis K,Zarogoulidis P,et al.Airway inflammation of chronic obstructive pulmonary disease[J].Thorac Dis,2014,6(S1):167-172 [br/] [4] Maniatis NA,Sfika A,Nikitopoulou I,et al. Acid-induced acute lung injury in mice is associated with P44/42 and c-Jun N-terminal kinase activation and requires the function of tumor necrosis factor α receptor I[J].Shock,2012,38(4):381-386. [br/] [5] Makabe H,Kojika M,Takahashi G,et al.Interleukin-18 levels reflect the long-term prognosis of acute lung injury and acute respiratory distress syndrome[J].J Anesth,2012,26(5):658-663. [br/] [6] Deshpande DA,Wang WC,Mcllmoyle EL,et al.Bitter taste receptors on airway smooth muscle bronchodilate by localized calcium signaling and reverse obstruction[J].Nat med,2010,16(11):1299-1304. [br/] [7] Scully M,Gang C,Condron C,et al.Protective role of cyclooxygenase(COX)-2 in experimental lung injury:evidence of a lipoxin A4-mediated effect[J].J Surg Res,2012,175(1):176-184. [br/] [8] 李忆兰,戴富林,武凡,等.三七皂甙Rb1对急性呼吸衰竭大鼠的作用及作用机制的研究[J].环球中医药杂志,2015,8(2):144-148. [br/] [9] De-Luca D,Baroni S,Vento G,et al.Secretory phospholipase A2 and neonatal respiratory distress: pilot study on broncho-alveolar lavage[J].Intensive care med,2008,34(10):1858-1864. [br/] [10] 李剑瑜,刘鹏年,武凡,等.三七皂甙Rg1对脂多糖诱导的大鼠肝细胞损伤的防护作用[J].环球中医药杂志,2012,5(5):19-22.

相似文献/References:

[1]孙爱华,张纾难.穴位贴敷联合肺康复锻炼对慢性阻塞性肺疾病稳定期患者生存质量的影响[J].环球中医药,2017,10(04):389.[doi:10.3969/j.issn.1674-1749.2017.04.002]
　SUN Aihua,ZHANG Shunan..Study on the effects of combine acupoint application with pulmonary rehabilitation exercise on the quality of life in patients with stable chronic obstructive pulmonary disease[J].,2017,10(07):389.[doi:10.3969/j.issn.1674-1749.2017.04.002]
[2]刘克锋 张艳玲 谭勇文 王慧贤 黄东晖.益气化痰止咳方配合中药浴足治疗COPD稳定期的临床效果观察[J].环球中医药,2017,10(07):758.[doi:10.3969/j.issn.1674-1749.2017.07.025]
[3]曹爱玲 何海浪 周贤梅.针刺治疗慢性阻塞性肺疾病疗效的Meta分析[J].环球中医药,2017,10(07):799.[doi:10.3969/j.issn.1674-1749.2017.07.038]
　CAO Ailing,HE Hailang,ZHOU Xianmei..Meta analysis of the therapeutic effect of acupuncture on chronic obstructive pulmonary disease[J].,2017,10(07):799.[doi:10.3969/j.issn.1674-1749.2017.07.038]

备注/Memo

备注/Memo:
作者简介:刘鹏年(1978- ),本科,主管检验师。研究方向:临床检验。E-mail:15932235609@163.com 通讯作者:李忆兰(1977- ),女,本科,主治医生。研究方向:细胞的损害与抗损害。E-mail:lpn78@163.com
更新日期/Last Update: 1900-01-01