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[1]李秋萍,蒋俏丽,翟文文,等.姜黄素-胡椒碱复方自微乳结肠稳定性研究[J].环球中医药,2015,8(10):1158-1163.[doi:10.3969/j.issn.1674.1749.2015.10.002]
　LI Qiu ping,JIANG Qiao li,ZHAI Wen wen,et al.Study on the colonic stability of curcuminpiperine dual drug loaded selfmicroemulsifying drug delivery system[J].,2015,8(10):1158-1163.[doi:10.3969/j.issn.1674.1749.2015.10.002]
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姜黄素-胡椒碱复方自微乳结肠稳定性研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年10期
页码:
1158-1163
栏目:
论著
出版日期:
2015-10-06

文章信息/Info

Title:
Study on the colonic stability of curcuminpiperine dual drug loaded selfmicroemulsifying drug delivery system
作者:
李秋萍; 蒋俏丽; 翟文文; 戴俊东
100102北京中医药大学中药学院［李秋萍、蒋俏丽、翟文文（硕士研究生）、戴俊东］
Author(s):
LI Qiuping; JIANG Qiaoli; ZHAI Wenwen; et al.
School of Pharmacy，Beijing University of Chinese Medicine，Beijing 100102，China
关键词:
姜黄素; 胡椒碱; 复方自微乳药物制剂; 人工结肠液; 小鼠结肠组织匀浆液; 稳定性
Keywords:
Curcumin; Piperine; Compound selfmicroemulsifying pharmaceutical formulations; Artificial colon fluid; The mice colonic tissue homogenized mixture; Stability
分类号:
R283
DOI:
10.3969/j.issn.1674.1749.2015.10.002
文献标志码:
A
摘要:
目的考察姜黄素—胡椒碱复方自微乳给药系统对姜黄素在结肠部位稳定性的改善作用，为其治疗结肠炎或结肠癌等结肠局部疾病提供科学依据。方法通过HPLC测定姜黄素的含量，37 ℃体外考察并比较姜黄素溶液、姜黄素—胡椒碱混合溶液及姜黄素—胡椒碱复方自微乳在人工结肠液及小鼠结肠组织匀浆液中的稳定性。结果37 ℃人工结肠液中孵育24小时后，姜黄素溶液、姜黄素—胡椒碱混合溶液和姜黄素—胡椒碱复方自微乳中姜黄素的降解百分率分别为1993%、1267%和1050%，降解过程符合一级动力学方程，半衰期分别为1967小时、5787小时和7523小时；37 ℃小鼠结肠组织匀浆液中孵育8小时后，姜黄素溶液、姜黄素—胡椒碱混合溶液和姜黄素-胡椒碱复方自微乳中姜黄素的降解百分率分别为1996%、1449%和463%。结论姜黄素-胡椒碱复方自微乳给药系统可以显著改善姜黄素在结肠部位的的稳定性（P＜005），有望增强对结肠局部疾病的治疗效果。
Abstract:
ObjectiveTo investigate whether the curcuminpiperine dual drug loaded selfmicroemulsifying drug delivery system(CurPIPSMEDDS) could enhance the curcumins stability in the colon site. This study could provide scientific basis for the treatment of colonic disease, such as colitis and colon cancer. MethodsHPLC method was established for the determination of the content of curcumin. To determine whether CurPIPSMEDDS is more stable in the colonic site, free curcumin, curcuminpiperine mixture and CurPIPSMEDDS were incubated in the artificial colon fluid and the mice colonic tissue homogenized mixture at 37℃, and sampled periodically to determine the concentration of curcumin by HPLC.ResultsThe degradation percentage of curcumin in free curcumin solution, curcuminpiperine mixture, and CurPIPSMEDDS were 1993%, 1267% and 1050% respectively after incubation for 24 hours in the artificial colon fluid at 37℃. The degradation process of curcumin followed apparent firstorder kinetics equation, and the halflife were 1967 h, 5787 h and 7523 h respectively.The degradation percentage of curcumin in free curcumin solution, curcuminpiperine mixture, and CurPIPSMEDDS were 1996%, 1449% and 463% respectively. After incubation for 8 hours in the mice colonic tissue homogenized mixture at 37℃. ConclusionThe results showed that CurPIPSMEDDS could significantly enhance the stability of curcumin (P<005) in the colonic site, and may enhance the efficacy for the treatment of colonic disease.

参考文献/References:

［1］Wang YF，Zu JN，Li J，et al. Curcumin promotes the spinal cord repair via inhibition of glial scar formation and inflammation ［J］. Neurosci Lett，2014，560:5156.
［2］SinglaV，Mouli VP，Garg SK，et al. Induction with NCB02 (curcumin) enema for mildtomoderate distal ulcerative colitisA randomized，placebocontrolled，pilot study ［J］. J Crohns Colitis，2014，8(3):208214.
［3］Zhao WC，Zhang B，Liao MJ，et al. Curcumin ameliorated diabetic neuropathy partially by inhibition of NADPH oxidase mediating oxidative stress in the spinal cord ［J］. Neurosci Lett，2014，560:8185.
［4］Yu CW，Wei CC，Liao VH. Curcuminmediated oxidative stress resistance in Caenorhabditis elegans is modulated by age1，akt1，pdk1，osr1，unc43，sek1，skn1，sir2.1　and mev1 ［J］. Free Radic Res，2014，48(3):371379.
［5］Zhang L，Luo JX，Zhang MH，et al. Effects of curcumin on chronic，unpredictable，mild， stressinduced depressivelike behaviour and structural plasticity in the lateral amygdala of rats ［J］. Int J Neuropsychopharmacol，2014，17(5):793806.
［6］Sanmukhani J，Satodia V，Trivedi J，et al. Efficacy and Safety of Curcumin in Major Depressive Disorder: A Randomized Controlled Trial ［J］. Phytother Res，2014，28(4):579585.
［7］Wang P，Su C，Li R，et al. Mechanisms and Effects of Curcumin on Spatial Learning and Memory Improvement in APPswe/PS1dE9 Mice ［J］. J Neurosci Res，2014，92(2):218231.
［8］冯慧利，李瑞晟，王虹，等. 姜黄素对APP/PS1双转基因小鼠海马IRS1和pIRS1表达的影响 ［J］.中国中药杂志，2013，38(9):12901294.
［9］Lim TG1，Lee SY，Huang Z，et al. Curcumin suppresses proliferation of colon cancer cells by targeting CDK2 ［J］. Cancer Prev Res (Phila)，2014，7(4):466474.
［10］郭立达，薛佳伟，渠开跃，等. 姜黄素联合奥沙利铂对人结肠癌 LoVo 细胞裸鼠移植瘤的抗癌作用研究［J］.中草药，2013，16:22612266.
［11］Zheng J，Wu C，Lin Z，et al. Curcumin upregulates phosphatase and tensin homologue deleted on chromosome 10 through microRNAmediated control of DNA methylationa novel mechanism suppressing liver fibrosis ［J］. FEBS J，2014，281(1):88103.
［12］Nabavi SF，Daglia M，Moghaddam AH，et al. Curcumin and Liver Disease: from Chemistry to Medicine ［J］.COMPR REV FOOD SCI F，2014，13(1):6277.
［13］Qureshi S，Shah AH，Ageel AM. Toxicity studies on Alpinia galanga and Curcuma longa ［J］. Planta Med，1992，58:124127.
［14］Shankar TN，Shantha NV，Ramesh HP，et al. Toxicity studies on turmeric (Curcuma longa):Acute toxicity studies in rats，guineapigs & monkeys ［J］. Indian J Exp Biol，1980，18(1):7375.
［15］Cheng AL，Hsu CH，Lin JK，et al. Phase I clinical trial of curcumin，a chemopreventive agent，in patients with highrisk or premalignant lesions ［J］. Anticancer Res，2001，21(4B):28952900.
［16］Lao CD，Rufn MT，Normolle D，et al. Dose escalation of a curcuminoid formulation ［J］. BMC Complement Altern Med，2006，6:1013.
［17］Prasad S，Tyagi AK，Aggarwal BB. Aggarwal，Recent Developments in Delivery，Bioavailability，Absorption and Metabolism of Curcumin: the Golden Pigment from Golden Spice ［J］. Cancer Res Treat，2014，46(1):218.
［18］Oliveira RG，AlencarFilho EB，Vasconcellos MLAA. The influence of piperine on the bioavailability of drugs: a molecular approach ［J］. Química Nova，2014，37(1):6973.
［19］Tu YS，Fu JW，Sun DM，et al. Preparation，characterisation and evaluation of curcumin with piperineloaded cubosome nanoparticles ［J］. J Microencapsul，2014，31(6):551559.
［20］Sharma V，Nehru B，Munshi A，et al. Antioxidant potential of curcumin against oxidative insult induced by pentylenetetrazol in epileptic rats ［J］. Methods Find Exp Clin Pharmacol，2010，32(4):227232.
［21］Suresh D，Srinivasan K.Tissue distribution & elimination of capsaicin，piperine & curcumin following oral intake in rats ［J］. Indian J Med Res，2010，131(5):682691.
［22］Shoba G，Joy D，Joseph T，et al. Influence of piperine on the pharmacokinetics of curcumin in animals and human volunteers ［J］. Planta Med，1998，64(4):353356.
［23］Wang Y J，Pan M H，Cheng A L，et al. Stability of curcumin in buffer solutions and characterization of its degradation products ［J］. J Pharm Biomed Anal，1997，15(12):18671876.
［24］韩刚，霍文，李秋影，等.姜黄素的稳定性研究［J］.中成药，2007，29(2):291293.
［25］韩刚，董月，原海忠，等.姜黄中脱甲氧基姜黄素和双脱甲氧基姜黄素对姜黄素稳定性的影响［J］.中草药，2009，40(9):14111413.
［26］韩刚，刘正猛，王晓雁，等.姜黄不同提取部位对姜黄素的稳定作用［J］.中国中药杂志，2007，32(10):915917.（收稿日期：20140902）

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更新日期/Last Update: 1900-01-01