[1]郭凤霞,陈路晓,刘斌,等.人参中人参皂苷类成分含量测定方法优化[J].环球中医药,2015,8(10):1175-1178.[doi:10.3969/j.issn.1674.1749.2015.10.006]
GUO Feng xia,CHEN Lu xiao,LIU Bin,et al.Improvement in determination method for Ginsenosides in Ginseng[J].,2015,8(10):1175-1178.[doi:10.3969/j.issn.1674.1749.2015.10.006]
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人参中人参皂苷类成分含量测定方法优化(
)
《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
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第8卷
- 期数:
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2015年10期
- 页码:
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1175-1178
- 栏目:
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论著
- 出版日期:
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2015-10-06
文章信息/Info
- Title:
-
Improvement in determination method for Ginsenosides in Ginseng
- 作者:
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郭凤霞; 陈路晓; 刘斌; 姜艳艳
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100102北京中医药大学中药学院
- Author(s):
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GUO Fengxia; CHEN Luxiao; LIU Bin; et al.
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School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
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- 关键词:
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-
- Keywords:
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Ginsenosides; Determination; Improvement
- 分类号:
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R284.1
- DOI:
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10.3969/j.issn.1674.1749.2015.10.006
- 文献标志码:
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A
- 摘要:
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目的优化人参中皂苷类成分人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量测定方法。方法采用单因素考察方式,对提取方式、提取溶剂、溶剂倍量和提取时间4个因素进行考察,采用HPLC法测定人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量,分析比较含量测定结果。结果人参中皂苷类成分含量测定供试品溶液制备最佳方法为采用50倍的70%甲醇回流提取人参2.5小时。结论本实验所建立的方法操作简单、准确可靠、重现性好,为人参皂苷的含量测定提供较优方法。【关键词】人参皂苷;含量测定;优化
- Abstract:
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ObjectiveTo improve the determination method of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in ginseng. MethodsFour factors including extraction method, extraction solvent, solvent volume and extraction time were studied through singlefactor test method. And HPLC method was adopted to analyze the determination results of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1. ResultsThe best sample preparation conditions for the content determination of ginsenosides were 50 times 70% methanol, reflux extraction and duration of 2.5 h. ConclusionsThe improved method is simple, accurate, and reproducible which could supply a better method for the content determination of ginsenosides.
参考文献/References:
[1]徐静,贾力,赵余庆.人参的化学成分与人参产品的质量评价\[J\].药物评价研究,2011,34(3):199203.
[2]国家药典委员会. 中华人民共和国药典(一部)\[M\].北京:中国医药科技出版社,2010:8.
[3]曲帅.人参有效活性成分的提取分离及含量测定\[D\].长春:吉林大学,2013.
[4]杨雨,郑斯文,金银萍,等.人参皂苷的提取分离方法研究进展\[J\].江苏农业科学,2014,42(5):214217.
[5]乔雪,李月茹.黑参中7种人参皂苷含量测定\[J\]. 人参研究,2012,24(1):1012.
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更新日期/Last Update:
1900-01-01