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[1]曹吴冰,高连印,车念聪,等.清毒汤对实验性重症肝损伤大鼠肿瘤坏死因子α及核转录因子κB表达的影响[J].环球中医药,2015,8(10):1186-1189.[doi:10.3969/j.issn.1674.1749.2015.10.009]
　CAO Wu bing,GAO Lian yin,CHE Nian cong,et al.Effects of Qingdu decoction on TNFα and NFκB expressions in the rats with experimental severe liver injury[J].,2015,8(10):1186-1189.[doi:10.3969/j.issn.1674.1749.2015.10.009]
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清毒汤对实验性重症肝损伤大鼠肿瘤坏死因子α及核转录因子κB表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年10期
页码:
1186-1189
栏目:
论著
出版日期:
2015-10-06

文章信息/Info

Title:
Effects of Qingdu decoction on TNFα and NFκB expressions in the rats with experimental severe liver injury
作者:
曹吴冰; 高连印; 车念聪; 张秋云; 杜宇琼; 付修文; 王融冰; 罗佳佳
100069 北京，首都医科大学中医药学院
Author(s):
CAO Wubing;  GAO Lianyin;  CHE Niancong;  et al.
Capital Medical University School of Traditional Chinese Medicine， Beijing 100069， China
关键词:
肝损伤; 内毒素; 硫代乙酰胺; 肿瘤坏死因子α; 核转录因子κB
Keywords:
Liver injury; Endotoxin; Thioacetamide; Tumor necrosis factor alpha; Nuclear factor kappa
分类号:
R285.5
DOI:
10.3969/j.issn.1674.1749.2015.10.009
文献标志码:
A
摘要:
目的观察清毒汤对实验性重症肝损伤大鼠内毒素（endotoxin，ET）、肿瘤坏死因子α（tumor necrosis factor alpha，TNFα）以及核转录因子κB（nuclear factor kappa B，NFκB）表达的影响，探讨该方治疗慢性重型肝炎内毒素血症的作用机制。方法建立硫代乙酰胺（thioacetamide，TAA）致实验性大鼠重症肝损伤模型，给予清毒汤干预后，检测大鼠ET、TNFα以及NFκB表达的水平。结果与正常组比较，模型组大鼠血清ET、TNFα以及NFκB蛋白含量水平均显著升高（P＜0.01）；与模型组比较，清毒汤中、低剂量组和乳果糖组ET、TNFα以及NFκB蛋白含量水平显著降低（P＜0.01），清毒汤高剂量组除ET外各指标均明显改善（P＜0.05）；与乳果糖组比较，清毒汤中剂量组ET及TNFα水平显著降低（P＜0.01），低剂量组ET及TNFα水平无明显差异（P＞0.05），高剂量组ET及TNFα水平明显高于乳果糖组（P＜0.05）。结论清毒汤对实验性肝损伤大鼠模型具有降低ET、TNFα以及NFκB蛋白水平的作用，尤其中剂量组效果最为明显，推测其分子机制可能为通过抑制NFκB因子的表达从而达到保肝目的。
Abstract:
ObjectiveTo observe the effects of Qingdu decoction on the expressions of endotoxin ( ET ), Tumor necrosis factor alpha ( TNFα ) and Nuclear factor kappa B ( NFκB ) in rats with severe liver injury and explore the mechanism of Qingdu decoction on treating Chronic severe hepatitis complicated with endotoxemia. MethodsThe severe liver injury rat model was induced by Thioacetamide ( TAA ), and treated with Qingdu decoction. And then the blood level of ET, TNFα and NFκB were measured. ResultsThe expression levels of ET, TNFα and NFκB in model group were significantly increased when compared to that of in normal group (P＜0.01 ). Compared to the model group, the expression levels of ET, TNFα and NFκB were decreased significantly in the middle and low dose Qingdu decoction groups and Lactulose group (P＜0.01 ). All indexes except ET in the high dose Qingdu decoction group were decreased significantly (P＜0.05 ). Compared to lactulose group, the expressions of ET and TNFα in the middle dose Qingdu decoction group were decreased significantly(P＜0.01 ), while the expressions of ET and TNFα in the low dose group showed no significantly difference (P＞0.05 ). The expressions of ET and TNFα in the high dose group were significantly higher than the Lactulose group (P＜005 ). ConclusionsQingdu decoction can improve liver injury and decrease ET, TNFα and NFκB expression levels in rats with hepatic injury complicated with endotoxemia, especially in the middle dose group. The mechanism could be related to the inhibition of NFκB activity.

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备注/Memo

备注/Memo:
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更新日期/Last Update: 1900-01-01