[1]宋芳娇,曾克武,屠鹏飞,等.加味五子衍宗方及其活性部位对脂多糖诱导 神经炎症反应的抑制作用及机制研究[J].环球中医药,2015,8(10):1190-1194.[doi:10.3969/j.issn.1674.1749.2015.10.010]
SONG Fang jiao,ZENG Ke wu,TU Peng fei,et al.Inhibitory effects of modified WuziYanzong prescription and its active components on LPSinduced inflammatory response on BV2 microglial cells[J].,2015,8(10):1190-1194.[doi:10.3969/j.issn.1674.1749.2015.10.010]
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加味五子衍宗方及其活性部位对脂多糖诱导 神经炎症反应的抑制作用及机制研究(
)
SONG Fang jiao,ZENG Ke wu,TU Peng fei,et al.Inhibitory effects of modified WuziYanzong prescription and its active components on LPSinduced inflammatory response on BV2 microglial cells[J].,2015,8(10):1190-1194.[doi:10.3969/j.issn.1674.1749.2015.10.010]
)《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
- 第8卷
- 期数:
- 2015年10期
- 页码:
- 1190-1194
- 栏目:
- 论著
- 出版日期:
- 2015-10-06
文章信息/Info
- Title:
- Inhibitory effects of modified WuziYanzong prescription and its active components on LPSinduced inflammatory response on BV2 microglial cells
- Author(s):
- SONG Fangjiao; ZENG Kewu; TU Pengfei; et al.
- Laboratory of Integrated Traditional Chinese and Western Medicine of First Hospital of Peking University，Beijing 100034，China
- Keywords:
- Neuroinflammation; BV2 microglia cell line; Modified WuziYanzong prescription; Antiinflammatory; Neural protection
- 分类号:
- R2855
- 文献标志码:
- A
- 摘要:
- 目的研究加味五子衍宗方及其活性部位对脂多糖诱导的BV-2小胶质细胞炎症反应的抑制作用及潜在机制。方法通过大孔树脂柱，将加味五子衍宗方乙醇总提取物洗脱分离得到水提组、20%醇提组、50%醇提组、70%醇提组和95%醇提组5个部位。针对无细胞毒性的部位研究了其对炎症因子一氧化氮和炎症蛋白诱导型一氧化氮合酶，环氧合酶2表达的影响，明确了抗炎活性部位，同时进一步研究了活性部位对核转录因子的激活以及活性氧表达的调控作用。结果70%醇提组和95%醇提组对细胞具有毒性，其余各部位(水提组，20%醇提组，50%醇提组)均无细胞毒性且表现出一定的抗炎活性。其中50%醇提组抗炎活性最优，甚至高于复方组。结论加味五子衍宗方的抗炎活性部位可能主要富集于50%醇提部分，其抗炎活性可能是通过抑制NFκB信号通路介导的炎症反应以及抗氧化应激实现的。
- Abstract:
- ObjectiveTo study the inhibitory effects of modified WuziYanzong prescription ( MWP ) and its active components on LPSinduced BV2 microglial cells and the potential mechanism. Methods70% ethanol extracts of MWP was separated by macro porous resin column, collecting water elution group, 20% alcohol elution group, 50% alcohol elution group, 70% alcohol elution group and 95% alcohol elution group. For the noncytotoxic components, we detected the expression of nitric oxide, inducible nitric oxide synthase and cyclooxygenase2 to identify the antiinflammatory active components. The regulatory effects of active components on nuclear factor κB ( NFκB ) activation and expression of reactive oxygen species(ROS) were also investigated. Results70% ethanol and 95% ethanol elution components were toxic to cells while the remaining components (water elution component，20% alcohol elution component，50% alcohol elution component) didnt show any cytotoxicity and exhibited some antiinflammatory activity. The 50% alcohol elution component is better than the other components or even higher than MWP. Conclusions50% alcohol extracts are responsible for the major antiinflammatory effective component of MWP and the antiinflammatory activity may be achieved through inhibiting NFκB signaling pathway and oxidative stress.
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