[1]艾则孜江·艾尔肯,田志浩,李莉,等.高效液相色谱法比较巴西桑叶与中国桑叶中芦丁含量[J].环球中医药,2015,8(11):1281-1383.[doi:10.3969/j.issn.1674-1749.2015.11.001]
Aizezijiang Ai er ken,TIAN Zhi hao,LI Li,et al.Comparison of rutin between Brazilian and Chinese mulberry leaf by HPLC[J].,2015,8(11):1281-1383.[doi:10.3969/j.issn.1674-1749.2015.11.001]
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高效液相色谱法比较巴西桑叶与中国桑叶中芦丁含量 (
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Aizezijiang Ai er ken,TIAN Zhi hao,LI Li,et al.Comparison of rutin between Brazilian and Chinese mulberry leaf by HPLC[J].,2015,8(11):1281-1383.[doi:10.3969/j.issn.1674-1749.2015.11.001]
)《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
- 第8卷
- 期数:
- 2015年11期
- 页码:
- 1281-1383
- 栏目:
- 巴西草药研究专题
- 出版日期:
- 2015-11-06
文章信息/Info
- Title:
- Comparison of rutin between Brazilian and Chinese mulberry leaf by HPLC
- 作者:
- 艾则孜江·艾尔肯; 田志浩; 李莉; 徐文英; 高山山; 韩丽; 赵百孝; 刘春生; 马长华
- 100102北京中医药大学中药学院(田志浩（博士研究生）、李莉(硕士研究生)、徐文英(硕士研究生)、高山山(硕士研究生)、刘春生、马长华)，针灸推拿学院(赵百孝)，中医养生研究所（韩丽）；新疆医科大学第一附属医院药学部（艾则孜江·艾尔肯）
- Author(s):
- Aizezijiang Aierken; TIAN Zhihao; LI Li; et al.
- School of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 100102， China
- Keywords:
- Mulberry leaves; Rutin; Content determination
- 分类号:
- R284
- 文献标志码:
- A
- 摘要:
- 目的通过研究不同产地桑叶中芦丁含量，为桑叶资源的开发、利用提供初步的基础。方法采用HPLC法分别对11份不同产地药材样品的芦丁含量进行测定。高效液相色谱法条件为TechMate C18(4.6 mm×250 mm，5 μm，NO.AKAD06833)，以甲醇-0.5%磷酸溶液为流动相进行梯度洗脱，检测波长为358 nm，柱温为30 ℃。结果8份国产样品的芦丁含量均未能达到《中华人民共和国药典》（一部）（2010年版）的含量标准，3份巴西样品高于《中华人民共和国药典》（一部）（2010年版）要求。结论国内桑叶中芦丁含量普遍较低，巴西黑桑叶中芦丁含量较高。
- Abstract:
- ObjectiveDecete the content of rutin in mulberry leaves from different origins by HPLC and analyze the discrepancy for the development and utilization of mulberry leaves provide a preliminary basis. Methods11 kinds of mulberry leaves samples from domestic and foreign were detected. HPLC conditions, C18 TechMate (4.6 mm× 250mm, 5μm, NO.AKAD06833) was used for detection. The gradient elution was carried out with methanol -0.5% phosphoric acid solution as mobile phase, the detection wavelength was 358 nm, and the column temperature was 30℃.ResultsThe content of rutin in 8 kinds of domestic samples was failed to achieve the standard of Chinese Pharmacopoeia (2010 edition), the Brazilian samples were higher than the requirements of pharmacopoeia. ConclusionThe content of rutin in domestic mulberry leaves is lower than that in Brazilian mulberry leaves, and the content in the Brazil black mulberry leaves is the most
参考文献/References:
［1]国家药典委员会.中华人民共和国药典（一部）［M］.北京：中国医药科技出版社，2010：279. [2]］江苏新医学院.中药大辞典（下册）[M].上海：上海科学技术出版社，1985：1963. [3]］刘利，潘一乐.不同桑种桑叶总黄酮含量分析[J].农业工程科学，2008，24（1）：488491. [4]］Andallu B，Suryakantham V，Lakshmi Srikanthi B，et al. Effect ofm ulberry (Morus indica L.) therapy on plasma and erythrocyte membrane lipids in patients with type 2 diabetes [J].Clin Chim Acta，2001，314(12)：4753. [5]］Andallu B，Varadachary ulu NCh. Control of hyperglycemia and retardation of cataract by m ulberry (Morus indica L.) leaves in streptozotocin diabetic rats[J]. Indian J Exp Biol，2002，40(7)：791795. [6]］Doi K，Kojima T，Fujimoto Y. M ulberry leaf extract inhibits the oxidative modification of rabbit and human low density lipoprotein[J]. Biol Pharm Bull，2000，23(9)：10661071. [7]］Kim SY，Gao JJ，Kang HK. Two flavonoids from the leaves of Morusalba induce differentiation of the human promyelocytic leukemia(HL60) cell line[J]. Biol Pharm Bull，2000，23(4)：451455. [8]］郭小补，廖森泰，邹宇晓，等.不同桑品种的桑叶总黄酮含量与体外抗氧化活性的相关性[J].蚕业科学，2008，34(3)：381386. [9]］杨普香，管帮福，黎小萍，等.桑叶中黄酮类化合物、氨基酸、桑多酚的含量变化探讨[J].蚕桑茶叶通讯，2003，(112)：23. [10]孟磊，孙莲，张丽静，等.新疆桑叶中总黄酮含量的测定[J].中国民族民间医药杂志，2002，(s6)：176177. [11]江蔚欣，钦浩，何文顺.中药指纹图谱研究的重要性及注意事项[J].黑龙江医药，2008，21(3)：2527.
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