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[1]顾选,李妍芃,崔秀梅,等.基于DNA条形码—产地—形态分析联用的巴西草药CENTELHA ASIATICA（积雪草）的生药学研究[J].环球中医药,2015,8(11):1294-1297.[doi:10.3969/j.issn.1674-1749.2015.11.005]
　GU Xuan,LI Yan peng,CUI Xiu mei,et al.The pharmaceutical research on Brazilian herb CENTELHA ASITICA（Centella asiatica） based on a combined analysis of DNA barcodingoriginmorphology[J].,2015,8(11):1294-1297.[doi:10.3969/j.issn.1674-1749.2015.11.005]
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基于DNA条形码—产地—形态分析联用的巴西草药CENTELHA ASIATICA（积雪草）的生药学研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1294-1297
栏目:
巴西草药研究专题
出版日期:
2015-11-06

文章信息/Info

Title:
The pharmaceutical research on Brazilian herb CENTELHA ASITICA（Centella asiatica） based on a combined analysis of DNA barcodingoriginmorphology
作者:
顾选; 李妍芃; 崔秀梅; 王娟; 韩丽; 赵百孝; 刘春生; 马长华; 崔馨云
100102北京中医药大学中药学院[李妍芃(硕士研究生)、王娟(硕士研究生)、刘春生、马长华、崔馨云（本科生）]；针灸推拿学院(赵百孝)，中医养生学研究所（韩丽）；北京华邈中药工程技术开发中心（顾选、崔秀梅）
Author(s):
GU Xuan;  LI Yanpeng;  CUI Xiumei;  et al.
School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 100102， China
关键词:
巴西草药; 积雪草; 生药学; DNA条形码
Keywords:
Brazilian herb; Centella asiatica; Pharmacognosy; DNA barcoding
分类号:
R284
DOI:
10.3969/j.issn.1674-1749.2015.11.005
文献标志码:
A
摘要:
目的对巴西草药CENTELHA ASITICA进行生药学研究。方法采用DNA条形码技术对巴西草药的叶片提取DNA、扩增ITS序列、双向测序，采用相似度法进行物种鉴定，再结合产地信息和性状特征核对DNA条形码鉴定结果进行验证。结果巴西草药CENTELHA ASITICA与NCBI核酸数据库积雪草ITS序列达到最高相似度，相似度为99%，鉴定其为伞形科植物积雪草Centella asiatica(L.) Urban。在地理分布上，巴西草药CENTELHA ASITICA符合积雪草的分布特征；从形态分析上，样品与积雪草特征相符合。结论基于DNA条形码-产地-形态分析的鉴定方法能够对巴西草药CENTELHA ASITICA进行鉴定，鉴定该物种来源于伞形科植物积雪草Centella asiatica(L.) Urban的干燥全草。生药学研究能够为CENTELHA ASITICA品种鉴定及质量标准研究提供依据。
Abstract:
ObjectiveTo study Brazilian herbal CENTELHA ASITICA from the perspective of biological pharmacy. MethodsBy applying DNA barcoding technology, total genomic DNA was isolated from the materials, and nuclear DNA ITS sequences were amplified and sequenced；DNA fragments were collated and matched by using ContingExpress. Similarity identification of BLAST analysis was performed. Next, the DNA bar code was verified by the information of plant origin and morphology. Families and genera were identified by Molecular identification. ResultsITS sequence of Brazil herb CENTELHA ASITICA and Centella asiatica (L.) Urban was 99% identical. The results can be solidified by their similarities in geographical distribution and morphological features. ConclusionBased on DNA barcodingoriginmorphological analysis Brazilian herbal CENTELHA ASITICA was identified as Centella asiatica L.and biological pharmacy can provide the basis for the identification and quality standard of CENTELHA ASITICA varieties.

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更新日期/Last Update: 1900-01-01