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[1]李妍芃,顾选,马恺悦,等.基于DNA条形码—高效液相色谱联用的巴西草药SENE（番泻叶）的品种鉴定[J].环球中医药,2015,8(11):1301-1305.[doi:10.3969/j.issn.1674-1749.2015.11.007]
　LI Yan peng,GU Xuan,MA Kai yue,et al.Research of Brazilian herb SENE based on DNA barcodingHPLC combined method evaluation[J].,2015,8(11):1301-1305.[doi:10.3969/j.issn.1674-1749.2015.11.007]
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基于DNA条形码—高效液相色谱联用的巴西草药SENE（番泻叶）的品种鉴定()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1301-1305
栏目:
巴西草药研究专题
出版日期:
2015-11-06

文章信息/Info

Title:
Research of Brazilian herb SENE based on DNA barcodingHPLC combined method evaluation
作者:
李妍芃; 顾选; 马恺悦; 吴浩忠; 赵百孝; 刘春生; 马长华; 韩丽
100102北京中医药大学中药学院[李妍芃（硕士研究生）、马恺悦（硕士研究生）、吴浩忠、刘春生、马长华]，针灸推拿学院（赵百孝），中医养生学研究所（韩丽）；北京华邈中药工程技术开发中心（顾选）
Author(s):
LI Yanpeng;  GU Xuan;  MA Kaiyue;  et al.
School of Chinese Materia Medica, Beijing University of Chinese Medicine. Beijing 100102, China
关键词:
巴西草药; 番泻叶; DNA条形码; 高效液相色谱; 番泻苷
Keywords:
Brazilian herb; SENE; DNA barcoding; HPLC; Sennoside
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2015.11.007
文献标志码:
A
摘要:
目的对巴西草药番泻叶SENE进行品种鉴别，进而研究其中番泻苷A和番泻苷B的含量并与中国番泻叶番泻苷A、B的比较，为其品种整理、临床使用及资源开发提供参考。方法利用DNA条形码—产地—形态分析联用技术鉴定巴西草药番泻叶SENE的基原，高效液相色谱技术测定番泻苷A和番泻苷B的含量。 结果品种鉴定结果为番泻叶，其中番泻苷A和番泻苷B两者的平均总含量为巴西番泻叶(1.56%)>中国番泻叶(1.52%)>2010年版《中华人民共和国药典》（一部）的含量要求(1.10%)。 结论巴西草药番泻叶来源于番泻叶Senna alexandrina Mill.，其番泻苷A和番泻苷B的含量与中国番泻叶差异不显著，且能达到药典要求。
Abstract:
ObjectiveTo identify the species of Brazilian herb SENE and study on the content of sennoside A and sennoside B, then to compare the results with Chinese Sennae folium, and provide reference for varieties systematics, clinical use and resource exploitation. MethodsThe source of varieties of Brazilian herb SENE was identified by a technique combined DNA barcoding, geographical origin and morphological features, and the content of sennoside A and sennoside B was analyzed by HPLC. ResultsDNA barcoding identification indicated that SENE was Senna alexandrina, and its average total content of sennoside A and sennoside B (1.56%) was higher than its Chinese counterpart (1.52%) and also higher than what is required in Chinese Pharmacopoeia (1.10%). ConclusionBrazilian herb SENE derives from Senna alexandrina Mill., and its sennoside A and sennoside B content have no significant difference with its Chinese counterpart which meets pharmacopoeia requirement.

参考文献/References:

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更新日期/Last Update: 1900-01-01