|本期目录/Table of Contents|

[1]余春霞,臧艺玫,顾选,等.基于性状—显微—DNA条形码联用的巴西牛至属植物药HORTELA基原鉴定研究[J].环球中医药,2015,8(11):1309-1311.[doi:10.3969/j.issn.1674-1749.2015.11.009]
　YU Chun xia,ZANG Yi mei,GU Xuan,et al.The variety identification of HORTELA in Brazil based on a combined analysis of macroscopicmicroscopicDNA barcoding[J].,2015,8(11):1309-1311.[doi:10.3969/j.issn.1674-1749.2015.11.009]
点击复制

基于性状—显微—DNA条形码联用的巴西牛至属植物药HORTELA基原鉴定研究()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1309-1311
栏目:
巴西草药研究专题
出版日期:
2015-11-06

文章信息/Info

Title:
The variety identification of HORTELA in Brazil based on a combined analysis of macroscopicmicroscopicDNA barcoding
作者:
余春霞; 臧艺玫; 顾选; 吴浩忠; 马长华; 韩丽; 刘春生; 赵百孝
450004郑州，河南省药品审评认证中心（余春霞）；北京中医药大学中药学院[臧艺玫（硕士研究生）、顾选、吴浩忠、刘春生、马长华]，针灸推拿学院（赵百孝），中医养生学研究所 [韩丽(硕士研究生)]； 北京华邈中药工程技术开发中心（顾选）
Author(s):
YU Chunxia;  ZANG Yimei;  GU Xuan;  et al.
Henan Province for Drug Evaluation Certification Center, Zhengzhou 450004, China
关键词:
牛至属; 基原鉴定; DNA条形码
Keywords:
Origanum Linn.; Variety identification; DNA barcoding
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2015.11.009
文献标志码:
A
摘要:
目的利用性状—显微—DNA条形码联用的方法对巴西牛至属植物药HORTELA进行基原鉴定研究。方法应用性状鉴定的方法初步对药材进行真伪鉴别；再应用显微鉴定的方法验证性状鉴定结果；最后提取样品DNA，扩增ITS序列，利用NCBI数据库中的BLAST功能分析相似度，对样品品种进行DNA条形码鉴定。结果性状—显微—DNA条形码综合分析结果表明，该样品为牛至属植物Origanum elongatum(Bonnet) Emb. & Maire.的干燥地上部分。结论HORTELA可能为多基原植物类药材，其来源为牛至属Origanum elongatum (Bonnet) Emb. & Maire.和薄荷属Mentha rotundifolia (Linn.) Huds.干燥地上部分。
Abstract:
ObjectiveTo study the variety identification of HORTELA of Origanum Linn. in Brazil with a combined analysis of macroscopicmicroscopicDNA barcoding. MethodsMacroscopic identification was used to identify the authenticity of samples preliminarily; then the results was verified by microscopic identification; finally, ITS sequences were amplified by PCR and sequenced, and BLAST in NCBI (basic local alignment search tool) database was used to calculate the similarity of sequences to identify variety of samples. ResultsThe combined analysis of macroscopicmicroscopicDNA barcoding indicated that Hortela collected from market in Brazil is the dried overground part of Origanum elongatum(Bonnet) Emb. & Maire. ConclusionHORTELA may be a multisource herb ,which originates from the dried overground part of Origanum elongatum(Bonnet) Emb. & Maire and Mentha rotundifolia (Linn.) Huds.

参考文献/References:

［1]季峰.浅谈中药的性状鉴定[J].国医论坛,2006,21(6):48.
[2]]杜晔.诠释中药鉴定四大鉴定方法[J].中国医药科学,2012,2(5):101102.
[3]]黄璐琦,肖培根,郭兰萍,等.分子生药学：一门新兴的边缘学科[J].中国科学C辑:生命科学,2009,39（12）:11011110.
[4]]冯杉杉,郑司浩,李亚康,等.中药材威灵仙及其伪品DNA条形码鉴别研究[J].药学学报,2014,49(2):260266.
[5]]许亮,刘春生,杨燕云,等.DNA条形码技术鉴定一种植物样品的研究[J].中国实验方剂学杂志,2014,20(7):217219.
[6]]叶子,卢叶,王峥涛,等.基于ITS序列鉴别石斛类药材[J].中国中药杂志,2014,39(20):39303935.
[7]]俞桂新,周荣汉.国产野生薄荷挥发油化学组分变异及其化学型[J].植物资源与环境,1998,7(3): 1318.
[8]]夏秋香,李慧,白红彤,等.6种薄荷属植物精油含量及成分的研究[J].园艺学报, 2012,39(S1):2756.
[9]]栾世杰. 薄荷寒热药性考辨[J]. 环球中医药, 2013, 6(8): 610612.
[10]孙丽娟,刘红兵,范文乾,等.牛至的化学成分研究[J].中草药,2007,33(12):17821785.
[11]朱斌,程向炜,刘迎新.牛至油化学成分、药理活性及提取方法研究进展[J].中药材,2007,30(8):10381041.
[12]D’Antuono L F, Galletti G C, Bocchini P. Variability of essential oil content and composition of Origanum vulgare L. populations from a North Mediterranean area (Liguria region, Northern Italy)[J]. Annals of Botany, 2000, 86(3): 471478.
[13]Hkarenral M. Reichling J, Geiss H K, et al.Comparative study on the in vitro antibacterial activity of Austrilian tea tree oil,cajeput oil,niaouli oil,Manuka oil,kanuka oil,and eucalyptus oil[J].Pharmazie,1999,54(6):460.
[14]周艳艳.牛至浸膏对肠道运动及解热镇痛作用的实验研究[J].湖北中医学院学报,2001,3(3):2224.

相似文献/References:

[1]臧艺玫,顾选,余春霞,等.基于显微—DNA条形码—产地分析联用的巴西柑橘属植物药FARINHA DE LARANJA（枳壳）基原鉴定[J].环球中医药,2015,8(11):1306.[doi:10.3969/j.issn.1674-1749.2015.11.008]
　ZANG Yi mei,GU xuan,YU Chun xia,et al.The variety identification of FARINHA DE LARANJA powder in Brazil based on a combined analysis of microscopicDNA barcodingorigin[J].,2015,8(11):1306.[doi:10.3969/j.issn.1674-1749.2015.11.008]
[2]臧艺玫,顾选,余春霞,等.基于DNA条形码—产地—形态分析联用的巴西马钱属植物药QUINA基原鉴定[J].环球中医药,2015,8(11):1312.[doi:10.3969/j.issn.1674-1749.2015.11.010]
　ZANG Yi mei,GU Xuan,YU Chun xia,et al.The variety identification of QUINA in Brazil based on a combined analysis of DNA barcodingoriginmorphology[J].,2015,8(11):1312.[doi:10.3969/j.issn.1674-1749.2015.11.010]

备注/Memo

备注/Memo:
-
更新日期/Last Update: 1900-01-01