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[1]臧艺玫,顾选,余春霞,等.基于DNA条形码—产地—形态分析联用的巴西马钱属植物药QUINA基原鉴定[J].环球中医药,2015,8(11):1312-1315.[doi:10.3969/j.issn.1674-1749.2015.11.010]
　ZANG Yi mei,GU Xuan,YU Chun xia,et al.The variety identification of QUINA in Brazil based on a combined analysis of DNA barcodingoriginmorphology[J].,2015,8(11):1312-1315.[doi:10.3969/j.issn.1674-1749.2015.11.010]
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基于DNA条形码—产地—形态分析联用的巴西马钱属植物药QUINA基原鉴定()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1312-1315
栏目:
巴西草药研究专题
出版日期:
2015-11-06

文章信息/Info

Title:
The variety identification of QUINA in Brazil based on a combined analysis of DNA barcodingoriginmorphology
作者:
臧艺玫; 顾选; 余春霞; 肖瑶; 赵百孝; 刘春生; 马长华; 韩丽
100102北京中医药大学中药学院[臧艺玫（硕士研究生）、顾选、肖瑶、刘春生、马长华]，针灸推拿学院（赵百孝），中医养生学研究所（韩丽）；北京华邈中药工程技术开发中心（顾选）；河南省药品审评认证中心（余春霞）
Author(s):
ZANG Yimei;  GU Xuan;  YU Chunxia; et al.
School of Chinese Materia Medica, Beijing University of Chinese Medicine. Beijing 100102, China
关键词:
马钱属; ITS序列; 基原鉴定
Keywords:
Strychnos Linn.; ITS sequence; Variety identification
分类号:
R284.1
DOI:
10.3969/j.issn.1674-1749.2015.11.010
文献标志码:
A
摘要:
目的通过DNA条形码—产地—形态分析联用的方法对巴西马钱属植物药QUINA进行基原鉴定研究，为这种药材的开发利用提供依据。方法通过提取样品 DNA、PCR扩增及双向测序，获得样品的内转录间隔区(internal transcribed spacer，ITS)序列，利用NCBI数据库中的BLAST功能计算相似度，对其基原进行DNA条形码鉴定；根据其产地信息对DNA条形码鉴定结果进行筛选和分析；依据其形态特征验证鉴定结果。 结果本次收集的植物类药材QUINA来源于马钱科马钱属植物Strychnos pseudo quina A.St.Hil.的根皮。结论DNA条形码—产地—形态三者联用的方法可弥补单一鉴定方法的不足，能够快速、准确的对未知样品进行基原鉴定。
Abstract:
ObjectiveTo study the variety identification of QUINA of Strychnos Linn. with the combined analysis of DNA barcodingoriginmorphology and provide an evidence for the exploitation of this kind of herbal in Brazil. MethodsITS sequences were amplified by PCR from genomic DNA and sequenced., BLAST(basic local alignment search tool) in NCBI database was used to calculate the similarity of sequences to identify the variety of samples; then the DNA barcoding identification results were screened and analyzed in reference to its origin information; finally, the morphological characteristics of samples was used to verify the accuracy of identification. ResultsQUINA collected from market in Brazil was the root bark of Strychnos pseudo quina A.St.Hil. ConclusionThe combined analysis of DNA barcodingoriginmorphology could make up for the deficit of onefold identification method. It contributes to identifying the variety of unidentified samples quickly and accurately.

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更新日期/Last Update: 1900-01-01