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[1]徐文英,马恺悦,冯孟鑫,等.基于“二维特征鉴别法”的巴西草药GENGIBRE真伪鉴别[J].环球中医药,2015,8(11):1320-1323.[doi:10.3969/j.issn.1674-1749.2015.11.012]
 XU Wen ying,MA Kai yue,FENG Meng xin,et al.Research on authentication of Brazilian herb GENGIBRE based on “Two Dimensional Feature Identification Method”[J].,2015,8(11):1320-1323.[doi:10.3969/j.issn.1674-1749.2015.11.012]
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基于“二维特征鉴别法”的巴西草药GENGIBRE真伪鉴别()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1320-1323
栏目:
巴西草药研究专题
出版日期:
2015-11-06

文章信息/Info

Title:
Research on authentication of Brazilian herb GENGIBRE based on “Two Dimensional Feature Identification Method”
作者:
徐文英马恺悦冯孟鑫顾选李妍芃赵百孝刘春生马长华韩丽
100102 北京中医药大学中药学院[徐文英(硕士研究生)、马恺悦(硕士研究生)、冯孟鑫(硕士研究生)、李妍芃(硕士研究生)、刘春生、马长华)],针灸推拿学院(赵百孝),中医养生研究所(韩丽);北京华邈中药工程技术开发中心(顾选)
Author(s):
XU WenyingMA KaiyueFENG Mengxinet al.
School of Chinese Materia Medica, Beijing University of Chinese Medicine,Beijing 100102,China
关键词:
巴西草药干姜特征图谱DNA条形码6姜辣素
Keywords:
Brazilian herbZingiberis rhizomaCharacteristic patternsDNA barcoding6Gingerol
分类号:
R284
DOI:
10.3969/j.issn.1674-1749.2015.11.012
文献标志码:
A
摘要:
目的对巴西草药GENGIBRE进行真伪鉴别。方法利用“二维特征鉴别法”,即DNA条形码技术联用HPLC技术的方法,鉴定巴西草药GENGIBRE的基原,并测定其6姜辣素的含量,对样品进行遗传学与化学两方面的鉴别。结果通过DNA条形码初步鉴定巴西草药GENGIBRE为芸香科植物,且与芸香科吴茱萸植物相似度最高,相似度范围为97%~99%;巴西草药GENGIBRE中6姜辣素的含量远低于中国干姜,分别为1.12 mg/g和7.61 mg/g。结论本试验应用“二维特征鉴别法”,在遗传学和化学两方面均证明所用巴西草药GENGIBRE相对于中国干姜是伪品。该方法在对未知样品进行某品种的定向真伪鉴别方面具有潜在的应用价值。
Abstract:
ObjectiveTo establish a method for identifying the authenticity of Brazil herb GENGIBRE. Methods“Two dimensional feature identification method”, that is,the DNA barcoding technology combined with HPLC,the method was used to identify the botanical origin of Brazil herb GENGIBRE and detect the content of 6gingerol in it . ResultsPreliminary identification of the Brazil herb TANCHAGEM with DNA barcoding method showed it was belong to Rutaceae and the similarity range between TANCHAGEM and Fructus evodiae is 97%~99%. The content of 6gingerol in Brazil GENGIBRE was far below that in Chinese ginger, respectively 1.12mg/g and 7.61mg/g. ConclusionIn this study, we applied the “twodimensional feature identification method” to show that the Brazilian herbal GENGIBRE is counterfeit goods respect to Chinese ginger in genetics and chemistry The method has potential application value in the identification of a variety of unknown samples.

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相似文献/References:

[1]徐文英,马恺悦,冯孟鑫,等.基于“二维特征鉴别法”的巴西草药TANCHAGEM真伪鉴别[J].环球中医药,2015,8(11):1316.[doi:10.3969/j.issn.1674-1749.2015.11.011]
 XU Wen ying,MA Kai yue,FENG Meng xin,et al.Research on authentication of Brazilian herb TANCHAGEM based on “Two Dimensional Feature Identification Method”[J].,2015,8(11):1316.[doi:10.3969/j.issn.1674-1749.2015.11.011]

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更新日期/Last Update: 1900-01-01