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[1]王宽,呼雪庆,黄玉萨,等.不同施灸方向对寒凝血瘀证大鼠血管收缩与舒张因子的影响[J].环球中医药,2016,9(01):2-6.[doi:10.3969/j.issn.1674-1749.2016.01.001]
　WANG Kuan,HU Xue-qing,HUANG Yu-sa,et al.Effects of different moxibustion-performed directions on the vascular contraction and relaxation factor on rats with cold gaused blood stasis syndrome[J].,2016,9(01):2-6.[doi:10.3969/j.issn.1674-1749.2016.01.001]
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不同施灸方向对寒凝血瘀证大鼠血管收缩与 舒张因子的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年01期
页码:
2-6
栏目:
论著
出版日期:
2016-01-06

文章信息/Info

Title:
Effects of different moxibustion-performed directions on the vascular contraction and relaxation factor on rats with cold gaused blood stasis syndrome
作者:
王宽; 呼雪庆; 黄玉萨; 王清然; 高春鹏; 刘民; 刘慧荣
200030 上海中医药大学上海市针灸经络研究所[王宽(硕士研究生)、刘慧荣],药物安全评价中心(呼雪庆、黄玉萨、王清然);
上海中医药大学岳阳临床医学院[刘民(硕士研究生)];
上海中医药大学联合培养研究基地苏州市中医院(高春鹏)
Author(s):
WANG Kuan;  HU Xue-qing;  HUANG Yu-sa;  et al.
Shanghai Institute of Acupuncture-Moxibustion and Meridian, Shanghai 200030, China
关键词:
艾灸;  方向;  寒凝血瘀证;  血浆内皮素-1;  一氧化氮
Keywords:
Moxibustion;  Direction;  Cold gaused blood stasis syndrome;  Endothelin-1;  Nitric oxide
分类号:
R245.81
DOI:
10.3969/j.issn.1674-1749.2016.01.001
文献标志码:
A
摘要:
目的 观察不同施灸方向对寒凝血瘀证模型大鼠血管收缩与舒张因子的影响。方法 选取清洁级Wistar大鼠32只,随机分组:正常组(A)、模型组(B)、顺经灸组(C)和逆经灸组(D),每组8只。在成功建立大鼠寒凝血瘀证模型后,对顺经灸和逆经灸两组大鼠实施不同操作方向的艾灸治疗,即沿膀胱经分别行“由上至下”及“由下至上”的单向反复操作,共治疗14天。治疗结束后,各组大鼠腹主动脉取血,采用酶联免疫吸附法检测大鼠血浆内皮素-1(endothelin-1, ET-1)及一氧化氮(nitric oxide, NO)的浓度。按照大鼠症状和体征量化评分表对大鼠的活动、毛发、大便、小便及脉络瘀血等情况进行相应评分,并最终计算大鼠寒凝血瘀的程度。结果 与正常组比较,模型组大鼠症状和体征量化积分及血浆ET-1浓度均明显升高(P<0.05),NO浓度明显下降(P<0.05); 与模型组比较,顺经灸组和逆经灸组大鼠症状和体征量化积分及血浆ET-1浓度均明显下降(P<0.05),NO浓度均明显升高(P<0.05); 顺经灸组大鼠症状和体征量化积分及血浆NO浓度较逆经灸组均有明显差异(P<0.05)。结论 顺经灸与逆经灸大鼠足太阳膀胱经均能明显促进寒凝血瘀证大鼠血浆NO的释放,抑制血浆ET-1的释放,改善大鼠寒凝血瘀症状,且顺经灸的治疗效应稍优于逆经灸。
Abstract:
Objective To observe the effects of different moxibustion-performed directions on the vascular contraction and relaxation factors in rats with cold gaused blood stasis syndrome. Methods 32 Wistar rats were divided into control group(A), model group(B), moxibustion performed along the meridian group(C)and moxibustion performed against the meridian group(D),8 rats in each group. After successful established the model of rats with cold gaused blood stasis syndrome, the moxibusition along or against the bladder meridian on rats were performed directionally and repeatedly, the treatment was for 14 days. After treatment, the levels of ET-1 and NO of the blood taken from rats' aortaventralis were detected by ELISA. According to the table of the quantificational score of symptom, observe the activities, hair, excrement, urinate and choroid blood stasis of rats, and calculate the degree of rats with cold gaused blood stasis syndrome. Results Compared with the A group, the quantificational score of symptom and the level of ET-1 in the B group were all increased with statistically significant difference(P<0.05)and the NO level was decreased significantly(P<0.05). Compared with the B group, the quantificational score of symptom and the level of ET-1 in the C and D group were reduced significantly(P<0.05), and the NO level increased apparently(P<0.05). The quantificational scores of symptom and NO levels in C and D group has statistical difference(P<0.05).Conclusions Both moxibustion groups can promote the NO release, inhibite the ET-1 release and improve the symptom in rats with cold gaused blood stasis syndrome. Moreover, the therapeutic effect of C group is better than D group.

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备注/Memo

备注/Memo:
基金项目: 国家重点基础研究发展(973计划)(2015CB554500); 上海中医药大学研究生“创新能力培养”专项
作者单位:200030 上海中医药大学上海市针灸经络研究所[王宽(硕士研究生)、刘慧荣],药物安全评价中心(呼雪庆、黄玉萨、王清然); 上海中医药大学岳阳临床医学院[刘民(硕士研究生)]; 上海中医药大学联合培养研究基地苏州市中医院(高春鹏)
作者简介:王宽(1989- ),2013级在读硕士研究生。研究方向:针灸治疗肠道疾病临床与基础研究。E-mail:wangkuan271@163.com
通讯作者:刘慧荣(1976- ),女,博士, 研究员,博士生导师。研究方向:针灸治疗肠道疾病临床与基础研究。E-mail:lhr_tcm@126.com
更新日期/Last Update: 2016-01-06