|本期目录/Table of Contents|

[1]李友山,杨博华,冀凌云.中药“蚓黄散”对大鼠糖尿病溃疡创面形态及促愈因子的影响[J].环球中医药,2016,9(01):10-14.[doi:10.3969/j.issn.1674-1749.2016.01.003]
　LI You-shan,YANG Bo-hua,JI Ling-yun..Effect of Yinhuangsan on morphology of ulcer wound and ulcer healing factors on diabetic rats[J].,2016,9(01):10-14.[doi:10.3969/j.issn.1674-1749.2016.01.003]
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中药“蚓黄散”对大鼠糖尿病溃疡创面形态及 促愈因子的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年01期
页码:
10-14
栏目:
论著
出版日期:
2016-01-06

文章信息/Info

Title:
Effect of Yinhuangsan on morphology of ulcer wound and ulcer healing factors on diabetic rats
作者:
李友山; 杨博华; 冀凌云
100700 北京中医药大学东直门医院周围血管科
Author(s):
LI You-shan;  YANG Bo-hua;  JI Ling-yun.
Department of peripheral vascular, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China
关键词:
蚓黄散;  创面愈合;  炎性因子;  生长因子;  晚期糖基化终末产物
Keywords:
Yinhuangsan;  Wound healing;  Inflammatory factor;  Growth factor;  Advanced glycasylation end products
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.01.003
文献标志码:
A
摘要:
目的 应用中药“蚓黄散”外治糖尿病足溃疡,观察其对创面组织愈合过程中的促进作用,探讨大鼠糖尿病溃疡愈合的作用机制。方法 建立大鼠糖尿病足溃疡模型后设模型组、西药组、中药组各10只,另设空白组10只,空白组及模型组创面不予干预,西药组予甲硝唑注射液外敷,中药组予“蚓黄散”外敷,治疗10天后检测大鼠血清中晚期糖基化终末产物(advanced glycasylation end products,AGEs)、炎性因子及生长因子含量; 增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、CD34染色分别检测创面肉芽组织中成纤维细胞数、新生毛细血管数; HE染色观察组织形态学变化。结果 与模型组比较,药物干预后大鼠血清中AGEs和肿瘤坏死因子-α、白细胞介素-1、C-反应蛋白均可降低,碱性成纤维因子、表皮细胞生长因子、血管内皮生长因子、血小板衍生生长因子水平均有所提高,创面肉芽组织中新生毛细血管数、成纤维细胞数均增多(P<0.05),而中药组均作用显著(P<0.05)。结论 “蚓黄散”可明显促进大鼠糖尿病溃疡创面的愈合,其机制可能与改善大鼠的炎性状态,提高大鼠血清中生长因子含量,降低AGEs水平,促进新生毛细血管、成纤维细胞的增殖有关。
Abstract:
Objective To study the role of traditional Chinese medicines(TCM)“Yinhuangsan” in the external treatment of diabetic foot ulcers by observing the process of the wound tissue healing and exploring the mechanism of the rat diabetic ulcer healing. Methods Diabetic foot ulcer model was established and the rats were randomly divided into model group, western medicine group and TCM group, 10 rats in each group, rats in all groups were respectively by gavage given oral no drug, metronidazole and Yinhuangsan. Another 10 normal rats were set as control group without drug treatment. After 10 days of treatment, the contents of AGEs, inflammatory factor and growth factor in serum were detected. Proliferating cell nuclear antigen(PCNA)staining and CD34 staining were respectively used to detect the number of fibroblast and number of newly born capillaries in granulation tissue. Hematoxylin and eosin(HE)staining was used to observe morphological changes. Results Compared with the model group, the content of AGEs, tissue necrosis factor-α(TNF-α), interleukin-1(IL-1)and C-reactive protein(CRP)were reduced after drug intervention, basic fibroblast growth factor(bFGF), endothelial growth factor(EGF), vascular endothelial growth factor(VEGF)and platelet derived growth factor(PDGF)were increased after drug intervention, newly born capillaries and fibroblasts were increased in granulation tissue(P<0.05), and the effect of TCM group was more pronounced than western medicine group(P<0.05). Conclusions Yinhuangsan can significantly promote the healing of ulcer wound in diabetes rats. The mechanism may be related to reducing inflammatory factor and AGEs in rat serum while improving growth factor, in rat serum, and promoting newly born capillaries and fibroblasts proliferation in the granulation tissue.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81302980)
作者单位: 100700 北京中医药大学东直门医院周围血管科
作者简介: 李友山(1979- ),博士,副主任医师。研究方向:中医外科周围血管病研究。E-mail:lys4626@sina.com
更新日期/Last Update: 2016-01-06