|本期目录/Table of Contents|

[1]杨婷,莫亚,陈莉苹,等.补虚化瘀法对豚鼠形觉剥夺性近视模型多焦视网膜电图的影响[J].环球中医药,2016,9(01):15-20.[doi:10.3969/j.issn.1674-1749.2016.01.004]
　YANG Ting,MO Ya,CHEN Li-ping,et al.Influences of traditional Chinese medicine of Buxu Huayu on the multifocal electroretinogram of a model of form deprivation myopia in guineas[J].,2016,9(01):15-20.[doi:10.3969/j.issn.1674-1749.2016.01.004]
点击复制

补虚化瘀法对豚鼠形觉剥夺性近视模型多焦 视网膜电图的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年01期
页码:
15-20
栏目:
论著
出版日期:
2016-01-06

文章信息/Info

Title:
Influences of traditional Chinese medicine of Buxu Huayu on the multifocal electroretinogram of a model of form deprivation myopia in guineas
作者:
杨婷; 莫亚; 陈莉苹; 黄秀蓉; 曹斌
610072 成都中医药大学研究生院[杨婷(硕士研究生)、曹斌(硕士研究生)];
成都中医药大学附属医院眼科(莫亚、黄秀蓉);
天津医科大学总医院滨海医院眼科(陈莉苹)
Author(s):
YANG Ting;  MO Ya;  CHEN Li-ping;  et al.
Graduate School of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China
关键词:
补虚化瘀;  豚鼠形觉剥夺近视模型;  多焦视网膜电图
Keywords:
Buxu Huayu;  Form deprivation myopia;  multfocal electroretiongram
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.01.004
文献标志码:
A
摘要:
目的 通过观察补虚化瘀法对豚鼠形觉剥夺性近视(form deprivation myopia,FDM)多焦视网膜电图(multifocal electroretinogram,mf ERG)的影响,探讨具有补虚化瘀功效的中药制剂八珍颗粒是否对形觉剥夺性近视豚鼠视网膜功能有影响。方法 将16只3周龄豚鼠随机分为两组:模型组、补虚化瘀组,每组各8只,右眼为剥夺眼,采用半透明眼罩遮盖诱导形觉剥夺性近视,左眼不予处理,为对照眼。补虚化瘀组予八珍颗粒混悬液1.12 g/(kg·d),每天同一时间灌胃1次,模型组予蒸馏水1 mL灌胃1次。所有动物在造模前与造模4周后进行带状检影,造模后进行多焦视网膜电图检查。结果(1)经过4周形觉剥夺,豚鼠右眼向近视转变,与造模前比较,豚鼠右眼近视屈光度有显著改变(P<0.05),而模型组右眼屈光度与补虚化瘀组右眼屈光度比较无明显差异(P>0.05)。(2)形觉剥夺4周后,模型组右眼1、2环N1波峰时, 1、2环P1峰时较模型组左眼延迟,差异有统计学意义(P<0.05); 模型组右眼总波、1、2、3环N1波反应密度,总波、1、2、3、4环P1波反应密度较模型组左眼显著降低, 差异有统计学意义(P<0.05); 补虚化瘀组右眼1环N1波峰时,总波、2环P1波峰时较模型组右眼延迟,差异有统计学意义(P<0.05)。中药制剂八珍汤颗粒组右眼总波、2、3环N1波反应密度,总波、1、2、3、4环P1波反应密度均较模型组右眼有所增加,差异有统计学意义(P<0.05)。 结论 补虚化瘀法有助于形觉剥夺性近视豚鼠视网膜mf ERG反应密度、峰时及其功能的恢复。
Abstract:
Objective To observe the effect of traditional Chinese medicine(TCM)of Buxu Huayu on the guinea of form deprivation myopia(FDM),and observe the effect of traditional Chinese medicine(TCM)of Bazhen granules on the retinal function of guinea of FDM.Methods 16 guineas were randomly divided into 2 groups: model control group(n=8)and Bazhen granules group(n=8), eight guineas each group.The right eyes of all guineas were form-deprived with semi-transparent membraes for 4weeks, and the left eyes were used as normal control without any treatment. Bazhen granules suspension1.12 g/(kg·d)was given for consecutive 4 weeks in the guineas of Buxu Huayu group,and distilled water was given for consecutive 4 weeks in the guineas of model control group. Before and 4 weeks after form deprivation, refraction was measured by retinoscopy after cycloplegia.after given drugs or distilled water for 4 weeks, to observe the effection by multifocal electroretinogram(mf ERG). Results After 4 weeks of form deprivation,the guinea pigs' s right eyes had myopic shift, and there was an approximately increase in FDM myopic refraction comparing to pre-models building; But the refraction was no obvious change between model control group and Bazhen decoction group after models builded in 4 weeks(P>0.05). The peak time of the N1 in the ring1-2 and the P1 in the total wave and ring1-2 in the model group' s right eyes was statistically increased compared with that of the left eyes of the model group(P<0.05). The amplitude densities of the N1 in the total wave,ring1-3 and the P1 in the total wave, ring1-4 in right eyes of model group was statistically decreased compared with that of left eye in the model group(P<0.05). The peak time of the N1 in the ring1 and the P1 in the total wave and ring 2 in the right eyes of Buxu Huayu group was significantly decreased compared with that of right eye in the model group(P<0.05). The amplitude densities of the N1 in the total wave,ring2-3 and the P1 in the total wave, ring1-4 in the right eye of Buxu Huayu group was statistically improved compared with that of right eye in the model group(P<0.05).Conclusion Buxu Huayu can protect the visual function of guineas model of FDM by improving amplitude density and decreasing peak time of mf ERG.

参考文献/References:

[1] Su-Kyung Jung,Jin Hae Lee,Hirohiko Kakizaki,et al.Prevalence of Myopia and its Association with Body Stature and Educational Level in 19-Year-Old Male Conscripts in Seoul, South Korea[J].Investigative Ophthalmology Vis Sci,2012,53(9):5579-5583.
[2] Song SF,LiH.Progress of epidemiologic study of myopia[J].Guo jiYan ke Zazhi( Int J Ophthalmol),2011,11(3):453-454.
[3] Jing Sun,Ji bo Zhou,Pei quan Zhao,et al.High Prevalence of Myopia and High Myopia in 5060 Chinese University Students in Shanghai[J].Invest Ophthalmol Vis Sci,2012,53(12):7504-7509.
[4] 葛坚.眼科学[M].北京:人民卫生出版社,2005.
[5] Zhao HL,Wang RQ,Wu MQ,et al.Dynamic changes of ocular biometric parameters: a modified form-deprivation myopia model of young guinea pigs[J]. Int J Ophthalmol,2011,(4):484-488.
[6] Ball S L,Petry H M.Noninvasive assessment of retinal function in rats using multifocal electro retinography[J].Invest Opthalmol Vis Sic,2000,41(12):610-617.
[7] Nusinowitz S,Hecken lively JR.Rod multifocal electroretinograms in mice[J].Invest Opthalmol Vis Sic,1999,40(12):284-285.
[8] 汪润芳.近视眼研究的现状与存在的问题[J].中华眼科杂志,2003,39(6):381-386.
[9] 中国学生体质与健康研究组.2000年中国学生体质与健康调研报告[M].北京:高等教育出版社,2002.
[10] Chan HL,Mohidin N.Variation of multifocal electroretinogram with axial length[J].Ophthalmic Physiol Optom,2003,(23):133.
[11] Luu CD,Lau AM,Lee S.Multifocal electroretinogram in adults and children with myopia[J].Arch Ophthalmol,2006,(124):328.
[12] 孙秀英,李建军,王钰,等.近视对多焦视网膜电图一阶反应的影响[J].中华眼底病杂志,2006,(22):103.
[13] Ho WC,Kee CS,Chan HH.Myopia progression in children is linked with reduced foveal mf ERG response[J].Invest Ophthalmol Vis Sci,2012,53(9):5320.
[14] Koh V,Tan C,Nah G,et al.Correlation of structural and electrophysiological changes in the retina of young high myopes[J].Ophthalmic Physiol Opt,2014,34(6):658.
[15] 莫亚,肖西立,何叶红,等,高度近视患者中医证候与年龄、视野的相关性研究[J].中国中医眼科杂志,2012,22(6)406-408.
[16] 杨蓓,刘桂香.豚鼠短期形觉剥夺行近视屈光度眼轴及巩膜改变[J].国际眼科杂志,2009,(9):1871-1875.
[17] Zhou X,Ye J,Willcox MD,et al.Changes in protein profiles of guinea pigs clear during development of form deprivation myopia and recovery[J].Mol Vis,2010,(16):2163-2174.
[18] Zhao HL,Wang RQ,Wu MQ,et al.Dynamic changes of ocular biometric parameters:a modified form-deprivation myopia model of young guinea pigs[J].International Journal of Ophthalmology,2011,(4):484-488.
[19] 吕梦,马东丽.近视眼动物模型的研究进展[J].国际眼科纵览,2013,(37):129-134.
[20] 周翔天,瞿佳.近视研究中动物模型的选择[J].中华眼科杂志,2005,41(6):486-487.
[21] 李巧莲,唐仁泓,易军晖.应用视网膜电图评估高度近视的视网膜功能改变[J].中外医学研究,2013,11(12):151-153.
[22] 罗俊.高度近视患者多焦视网膜电流图的评估[J].国际眼科杂志,2006,6(6):1339-1361.
[23] Mao X,Li X.Effects of bushenhuoxue on protecting the visual function of rat with chronic intraocular hypertension[J].Int J Ophthalmol(Guo ji Yan ke Za zhi),2010,10(2):238-240.
[24] HanSS,Shi YN.Clinical research on the correlation of visual functional status and diopter in high myopia[J].Guoji Yan ke Za zhi(Int EyeSci),2013,13(9):1857-1860.
[25] 潘洪平.八珍汤的药理研究和临床应用[J].中成药,2003,25(11):附2-附4.
[26] 张超,南莉莉.八珍汤物质基础及其药理学研究进展[J].上海医药,2008,29(6):273-276.
[27] 尹丽丽.人参的药理作用现代研究进展[J].中医临床研究,2013,5(5):122.
[28] 鹿庆,孙葆忱,王津津.人参皂苷Rg1、Rb1和维生素E对氧化损伤的牛细胞调亡的影响[J].眼科新进展.1999,19(3):152.
[29] 潘毓宁,潘洪平,吴隐雄.八珍汤对老龄大鼠血液流变学改善作用的研究[J].广西医学,1997,19(4):581-584.
[30] Gao RX,Li ZX.Study of multifocal electroretinogram in researching retinal function of myopia eyes[J].Rec Adv Ophthalmol,2012,32(3):273-275.

相似文献/References:

备注/Memo

备注/Memo:
基金项目: 四川省教育厅课题(11ZA065); 四川省卫生厅课题(110527); 成都中医药大学附属医院科技发展基金(2012-D-YY-12)
作者单位: 610072 成都中医药大学研究生院[杨婷(硕士研究生)、曹斌(硕士研究生)]; 成都中医药大学附属医院眼科(莫亚、黄秀蓉); 天津医科大学总医院滨海医院眼科(陈莉苹)
作者简介: 杨婷(1988- ),女,2013级在读硕士研究生。研究方向:中医药对视功能的保护。E-mail:815396209@qq.com
通讯作者: 莫亚(1971- ),女,博士,硕士生导师,主任医师。研究方向:中医药对视功能的保护。E-mail:myydcg@sohu.com
更新日期/Last Update: 2016-01-06