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[1]史云晖,柳洋,韦尼,等.活血解毒方对干燥综合征小鼠干扰素-γ、B细胞活化因子及其受体的干预作用研究[J].环球中医药,2016,9(01):20-24.[doi:10.3969/j.issn.1674-1749.2016.01.005]
　SHI Yun-hui,LIU Yang,WEI Ni,et al.Effect of Huoxue Jiedu formula on IFN-γ and BAFF/BAFF-R in NOD mice with Sjogren's syndrome[J].,2016,9(01):20-24.[doi:10.3969/j.issn.1674-1749.2016.01.005]
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活血解毒方对干燥综合征小鼠干扰素-γ、B细胞 活化因子及其受体的干预作用研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年01期
页码:
20-24
栏目:
论著
出版日期:
2016-01-06

文章信息/Info

Title:
Effect of Huoxue Jiedu formula on IFN-γ and BAFF/BAFF-R in NOD mice with Sjogren's syndrome
作者:
史云晖; 柳洋; 韦尼; 刘小平; 朱跃兰
100029 北京中医药大学研究生院[史云晖(博士研究生)、柳洋(硕士研究生)];
北京中医药大学东方医院风湿病科(韦尼、刘小平、朱跃兰)
Author(s):
SHI Yun-hui;  LIU Yang;  WEI Ni;  et al.
Graduate School of Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
干燥综合征;  B细胞活化因子;  干扰素;  中医;  活血解毒
Keywords:
Sjogren's syndrome;  B cell activating factor belonging to the TNF family;  Interferon;  Traditional Chinese medicine;  Huoxue Jiedu
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.01.005
文献标志码:
A
摘要:
目的 探索活血解毒方对干燥综合征小鼠干扰素(interferon,IFN)-γ、B细胞活化因子(B cell activating factor belonging to the TNF family, BAFF)及其受体BAFF-R的影响及其机制。 方法 以C57BL/6j小鼠及NOD/Ltj小鼠为实验动物,分别以蒸馏水、白芍总苷混悬液、活血解毒方汤剂灌胃干预,干预过程中观察小鼠一般状态,于干预60天后测量小鼠唾液流率,并取小鼠颌下腺及血清,测量颌下腺指数及血清中BAFF、BAFF-R、 IFN-γ及颌下腺BAFF、 BAFF-R水平。结果 正常组唾液流率均较其他各组高(P<0.05),实验第40天开始,对照组及治疗组的唾液流率开始较模型组升高(P<0.05),而对照组与治疗组之间差异不明显(P>0.05); 第60天对照组与治疗组之间差异明显(P<0.05)。颌下腺指数,正常组较模型组、对照组、治疗组高(P<0.05)。模型组较对照组、治疗组低(P<0.05),对照组与治疗组之间差异不明显(P>0.05)。血清BAFF、BAFF-R、IFN-γ及颌下腺BAFF、BAFF-R水平,正常组均较模型组、对照组、治疗组低(P<0.05),模型组较对照组、治疗组高(P<0.05),其中对照组水平高于治疗组(P<0.05),各组之间比较,差异具有统计学意义(P<0.05)。结论 活血解毒方可减轻NOD小鼠颌下腺炎症,增加唾液分泌量,降低血清中BAFF、 BAFF-R、IFN-γ及颌下腺BAFF、 BAFF-R的水平,从而达到治疗干燥综合征的作用。
Abstract:
Objective To observe the effect of Huoxue Jiedu formula on BAFF/BAFF-R in NOD mice and explore the mechanism of Huoxue Jiedu formula to Sjögren's syndrome. Methods C57BL/6j mice and NOD/Ltj mice were used for research. The mice were intervened with water, total glucosides of peaony and Huoxue Jiedu formula respectively. The general status was observed. After 60 days of intervenation, the flow rate of saliva, index of submandibular gland, BAFF, BAFF-R and IFN-γ in serum and BAFF, BAFF-R in salivary gland were tested. Results One-way ANOVA was used for statistical analysis. When compared between two groups, LSD was used. The flow rate of saliva of the normal group was higher than other groups in every time point(P<0.05). Since the 40th day, the flow rates of saliva of control group and treatment group was higher than that in model group. There was no significant difference between control group and treatment group at that time(P>0.05). At the 60th day, the flow rate in treatment group was significantly higher than that in control group(P<0.05). The index of submandibular gland of the normal group was the highest(P<0.05)while that was the lowest of the model group(P<0.05). There was no significantly difference between the treatment group and control group(P>0.05). As for BAFF, BAFF-R and IFN-γ in serum and BAFF, BAFF-R in salivary gland, the normal group was the lowest(P<0.05), the model group was the highest(P<0.05), the treatment group was significantly lower than the control group(P<0.05).Conclusions Huoxue Jiedu formula can alleviate the inflammation of salivary gland, increase saliva and reduce BAFF, BAFF-R and IFN-γ in serum and BAFF, BAFF-R in salivary gland.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:北京中医药大学校级自主课题(2014-JYBZZ-XS-147)
作者单位:100029 北京中医药大学研究生院[史云晖(博士研究生)、柳洋(硕士研究生)]; 北京中医药大学东方医院风湿病科(韦尼、刘小平、朱跃兰)
作者简介:史云晖(1989- ),女,2013级在读博士研究生。研究方向:中医药治疗风湿免疫病。E-mail:syh890501@163.com
通讯作者:朱跃兰(1959- ),女,博士,主任医师,博士生导师。研究方向:中医药治疗风湿免疫病。E-mail:zhuyuelanting@163.com
更新日期/Last Update: 2016-01-06